Establishment of HPLC fingerprints for <italic style="font-style: italic">Ardisia crenata</italic>， <italic style="font-style: italic">Sophora tonkinensis</italic> and their couplet medicines and content determination of 5 chemical components

CHEN Yun; SHI Hui; FENG Tingting; ZHANG Liyan; DONG Xiu; ZHANG Jinhe; HUANG Bei; ZHOU Ying

doi:10.6039/j.issn.1001-0408.2023.16.06

您当前的位置：

首页 >

文章列表页 >

Establishment of HPLC fingerprints for Ardisia crenata， Sophora tonkinensis and their couplet medicines and content determination of 5 chemical components

更新时间：2025-09-25

- Establishment of HPLC fingerprints for Ardisia crenata， Sophora tonkinensis and their couplet medicines and content determination of 5 chemical components
- ZHONGGUO YAOFANG Vol. 34, Issue 16, Pages: 1949-1954(2023)
- 作者机构：
  
  1.贵州中医药大学药学院，贵阳　550025
  2.贵州中医药大学药食两用资源应用与开发研究中心，贵阳　550025
  3.贵州三力制药股份有限公司，贵州安顺　561100
  4.贵州省药品监督管理局检查中心，贵阳　550025
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2023.16.06
  CLC： R917;R284.1
- Received：15 March 2023，
  
  Revised：2023-07-12，
  
  Accepted：26 July 2023，
  
  Published：30 August 2023
- 稿件说明：
移动端阅览
陈贇,石慧,俸婷婷,等.朱砂根、山豆根及其药对的HPLC指纹图谱建立及5种化学成分的含量测定[J].中国药房,2023,34(16):1949-1954.

CHEN Yun,SHI Hui,FENG Tingting,et al.Establishment of HPLC fingerprints for Ardisia crenata， Sophora tonkinensis and their couplet medicines and content determination of 5 chemical components[J].ZHONGGUO YAOFANG,2023,34(16):1949-1954.
陈贇,石慧,俸婷婷,等.朱砂根、山豆根及其药对的HPLC指纹图谱建立及5种化学成分的含量测定[J].中国药房,2023,34(16):1949-1954. DOI： 10.6039/j.issn.1001-0408.2023.16.06.

CHEN Yun,SHI Hui,FENG Tingting,et al.Establishment of HPLC fingerprints for Ardisia crenata， Sophora tonkinensis and their couplet medicines and content determination of 5 chemical components[J].ZHONGGUO YAOFANG,2023,34(16):1949-1954. DOI： 10.6039/j.issn.1001-0408.2023.16.06.

摘要

目的

建立朱砂根、山豆根及其药对的指纹图谱，同时测定其中5种成分的含量。

方法

以水为溶剂，结合冷冻干燥技术制备朱砂根、山豆根单煎冻干粉和朱砂根-山豆根药对合煎冻干粉样品；采用高效液相色谱（HPLC）法建立3种冻干粉样品的指纹图谱，并同法测定没食子酸等5种成分的含量。

结果

朱砂根、山豆根单煎冻干粉样品和朱砂根-山豆根药对合煎冻干粉样品分别有5、10、14个共有峰；与对照图谱的相似度均大于0.90；朱砂根-山豆根药对合煎冻干粉样品中，指认3号峰为没食子酸、4号峰为苦参碱、6号峰为氧化苦参碱、8号峰为岩白菜素、14号峰为三叶豆紫檀苷。朱砂根单煎冻干粉样品中，没食子酸、岩白菜素的平均含量分别为0.499 3、4.962 6 mg/g；山豆根单煎冻干粉样品中，苦参碱、氧化苦参碱、三叶豆紫檀苷的平均含量分别为3.046 0、2.336 6、0.278 6 mg/g；朱砂根-山豆根药对合煎冻干粉样品中，没食子酸、苦参碱、氧化苦参碱、岩白菜素、三叶豆紫檀苷的平均含量分别为0.560 6、2.548 7、1.382 2、5.960 7、0.279 1 mg/g，转移率为8.87%～513.19%。

结论

所建立指纹图谱及含量测定方法稳定、可行，可用于朱砂根、山豆根及朱砂根-山豆根药对的质量控制；朱砂根-山豆根药对合煎冻干粉样品中苦参碱、氧化苦参碱的平均含量降低。

Abstract

OBJECTIVE

To establish the fingerprints of

Ardisia crenata

，

Sophora tonkinensis

and their couplet medicines， and to determine the contents of five components in them.

METHODS

Using water as solvent， single lyophilized powder of

A. crenata

and

S. tonkinensis

and combined lyophilized powder of their couplet medicines were prepared by combining lyophilization technology. The fingerprints of three lyophilized powder samples were established by using high-performance liquid chromatography （HPLC）， and the contents of 5 kinds of components such as gallic acid were determined simultaneously.

RESULTS

There were 5， 10 and 14 common peaks in the fingerprints for single lyophilized powder of

A. crenata

and

S. tonkinensis

and combined lyophilized powder of their couplet medicines； the similarities of them with the control fingerprints were all greater than 0.90. For combined lyophilized powder of couplet medicines， peak 3 was identified as gallic acid， peak 4 as matrine， peak 6 as oxymatrine， peak 8 as bergenin， and peak 14 as trifolirhizin. In single lyophilized powder of

A. crenata

， the average contents of gallic acid and bergenin were 0.499 3 and 4.962 6 mg/g， respectively. In single lyophilized powder of

S. tonkinensis

， the average contents of matrine， oxymatrine and trifolirhizin were 3.046 0， 2.336 6 and 0.278 6 mg/g， respectively. In combined lyophilized powder of couplet medicines， the average contents of gallic acid， matrine， oxymatrine， bergenin and trifolirhizin were 0.560 6， 2.548 7， 1.382 2， 5.960 7 and 0.279 1 mg/g， respectively. The transfer rates were 8.87%-513.19%.

CONCLUSIONS

The established fingerprint and content determination methods are stable and feasible， and can be used for the quality control of

A. crenata

and

S. tonkinensis

and their couplet medicines. The average contents of matrine and oxymatrine in combined lyophilized powder of

A. crenata

S. tonkinensis

couplet medicines are decreased.

关键词

Keywords

references

叶晴，陈金鹏，凌悦，等 . 朱砂根化学成分和药理作用的研究进展 [J ] . 中草药， 2022 ， 53 （ 9 ）： 2851 - 2860 .

国家药典委员会 . 中华人民共和国药典：一部 [M ] . 2020年版 . 北京：中国医药科技出版社， 2020 ： 28 ， 143 - 144 .

HAN C C ， GUO J Y . Antibacterial and anti-inflammatory activity of traditional Chinese herb pairs， Angelica sinensis and Sophora flavescens [J ] . Inflammation ， 2012 ， 35 （ 3 ）： 913 - 919 .

CHAN B C L ， YU H ， WONG C W ， et al . Quick identification of kuraridin，a noncytotoxic anti-MRSA（methicillin-resistant Staphylococcus aureus ）agent from Sophora flavescens using high-speed counter-current chromatography [J ] . J Chromatogr B Analyt Technol Biomed Life Sci ， 2012 ， 880 （ 1 ）： 157 - 162 .

ZHOU Y H ， WU Y ， DENG L ， et al . The alkaloid matrine of the root of Sophora flavescens prevents arrhythmogenic effect of ouabain [J ] . Phytomedicine ， 2014 ， 21 （ 7 ）： 931 - 935 .

潘其明，黄日镇，潘英明，等 . 山豆根的化学成分研究 [J ] . 中国中药杂志， 2016 ， 41 （ 1 ）： 96 - 100 .

傅月朦，余登香，王淑娜，等 . 山豆根黄酮类成分药理作用及机制研究进展 [J ] . 中草药， 2022 ， 53 （ 19 ）： 6234 - 6244 .

高佩佩，王珍，刘静，等 . 氧化苦参碱的药代动力学、毒理学及药理作用 [J ] . 中国药理学通报， 2019 ， 35 （ 7 ）： 898 - 902 .

夏晓旦，普天磊，黄婷，等 . 岩白菜的化学成分、含量考察与药理作用研究概况 [J ] . 中国药房， 2017 ， 28 （ 16 ）： 2270 - 2273 .

艾芸 . 苦参配伍后化学成分变化的研究及丹参素衍生物的合成 [D ] . 西安：西北大学， 2006 .

郑丽娜，孙虎，谢元璋，等 . 山豆根化学成分与功效、毒性相互关系的研究进展 [J ] . 食品与药品， 2011 ， 13 （ 5 ）： 205 - 209 .

韩馥蔓 . 山豆根单煎汤剂及其复方汤剂的化学成分、急性毒性及抗炎活性比较 [D ] . 北京：中国中医科学院， 2017 .

Views

193

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Quality evaluation of Fuzheng capsules based on HPLC fingerprint and multi-ingredient content determination

Differential component analysis between Fructus Tritici Levis and Triticum aestivum based on qualitative and quantitative methods

Fingerprint establishment and content determination of Pulan liyan mixture

Comprehensive evaluation of the quality of Yindan huoxue tongyu granules based on fingerprint combined with content of pharmacodynamic components

Establishment of HPLC fingerprint and content determination of Gerbera delavayi

Related Author

LI Yunrong

NONG Luwei

LI Li

JIANG Xianzai

ZENG Cuiqiong

CHEN Song

WANG Liuping

HU Yu

Related Institution

Zhuang & Yao Medicine Research and Development Center， Guangxi International Zhuang Medicine Hospital Affiliated to Guangxi University of Chinese Medicine

College of Pharmacy， Guangxi University of Chinese Medicine

National Demonstration Center for Experimental Chinese Medicine Education， Guangxi University of Chinese Medicine

The Affiliated Hospital of Nanjing University of Chinese Medicine

The First Clinical Medical College， Nanjing University of Chinese Medicine

⁰

Establishment of HPLC fingerprints for Ardisia crenata， Sophora tonkinensis and their couplet medicines and content determination of 5 chemical components

DOI：10.6039/j.issn.1001-0408.2023.16.06

摘要

Abstract

关键词

Keywords

references