OBJECTIVE

2

To establish the methods for simultaneous determination of rutin， forsythiaside A， （+）-pinoresinol-4-

O

-

β

-D-glucopyranoside， forsythin and forsythigenin in

Forsythia suspensa

flower.

METHODS

2

UPLC method was adopted. The determination was performed on ACQUITY UPLC HSS T3 C

18

column with mobile phase consisted of acetonitrile-0.1% phosphoric acid solution （gradient elution） at the flow rate of 0.3 mL/min. The detection wavelengths were set at 275 nm （0-8 min），330 nm （8-10.5 min），275 nm （10.5-32 min）， respectively. The column temperature was 25 ℃， and sample size was 1 μL. Taking rutin as reference， the content of each component was determined by quantitative analysis of multi-components by single-marker （QAMS） method， and then compared with external standard method.

RESULTS

2

The contents of forsythiaside A， （+）-pinoresinol-4-

O

-

β

-D-glucopyranoside， forsythin and forsythigenin by QAMS were 7.472-7.671， 2.919-2.986， 1.439-1.486， 1.523-1.566 mg/g； the results obtained by the external standard method were 7.454-7.664， 2.913-2.996， 1.444-1.484， 1.519-1.562 mg/g， respectively. There was no significant difference in the measurement results between the two methods， with a relative deviation less than 1.0%.

CONCLUSIONS

2

This study successfully establishes the UPLC-QAMS method for simultaneous determination of five components in

F. suspensa

flower， and the results obtained by this method are not significantly different from those obtained by the external standard method. It can be used for quality control of

F. suspensa

flower.