Improvement effects and mechanism of total flavonoids of <italic style="font-style: italic">Bidens pilosa</italic> on lipopolysaccharide-induced neuroinflammation in mice

TANG Fengman; PANG Xiaojun; XIE Yan; LIU Chunfang

doi:10.6039/j.issn.1001-0408.2023.14.11

您当前的位置：

首页 >

文章列表页 >

Improvement effects and mechanism of total flavonoids of Bidens pilosa on lipopolysaccharide-induced neuroinflammation in mice

更新时间：2025-09-27

- Improvement effects and mechanism of total flavonoids of Bidens pilosa on lipopolysaccharide-induced neuroinflammation in mice
- ZHONGGUO YAOFANG Vol. 34, Issue 14, Pages: 1719-1723(2023)
- 作者机构：
  
  1.广西医科大学药学院，南宁　530021
  2.钦州市第二人民医院药学部，广西钦州　535000
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2023.14.11
  CLC： R965
- Received：27 January 2023，
  
  Revised：2023-06-19，
  
  Accepted：20 June 2023，
  
  Published：30 July 2023
- 稿件说明：
移动端阅览
唐丰曼,庞晓军,谢颜,等.鬼针草总黄酮对脂多糖诱导小鼠神经炎症的改善作用及机制[J].中国药房,2023,34(14):1719-1723.

TANG Fengman,PANG Xiaojun,XIE Yan,et al.Improvement effects and mechanism of total flavonoids of Bidens pilosa on lipopolysaccharide-induced neuroinflammation in mice[J].ZHONGGUO YAOFANG,2023,34(14):1719-1723.
唐丰曼,庞晓军,谢颜,等.鬼针草总黄酮对脂多糖诱导小鼠神经炎症的改善作用及机制[J].中国药房,2023,34(14):1719-1723. DOI： 10.6039/j.issn.1001-0408.2023.14.11.

TANG Fengman,PANG Xiaojun,XIE Yan,et al.Improvement effects and mechanism of total flavonoids of Bidens pilosa on lipopolysaccharide-induced neuroinflammation in mice[J].ZHONGGUO YAOFANG,2023,34(14):1719-1723. DOI： 10.6039/j.issn.1001-0408.2023.14.11.

摘要

目的

探讨鬼针草总黄酮（TFB）对脂多糖（LPS）诱导小鼠神经炎症的改善作用及机制。

方法

将50只C57BL/6小鼠随机分为正常对照组、LPS组和TFB低、中、高剂量组，每组10只。TFB低、中、高剂量组小鼠按60、120、240 mg/kg灌胃TFB溶液，正常对照组和LPS组小鼠灌胃等体积生理盐水，每日1次，连续给药21 d。从给药第15天起，除正常对照组外，其余组小鼠连续7 d腹腔注射LPS（400 μg/kg）建立神经炎症模型。末次给药4 h后麻醉小鼠取脑组织。观察小鼠神经元形态变化，检测小鼠脑组织中一氧化氮（NO）和肿瘤坏死因子α（TNF-α）、白细胞介素1β（IL-1β）、IL-6、IL-10的含量，检测小鼠脑组织中炎症通路相关蛋白[诱导型NO合酶（iNOS）、环氧合酶2（COX-2）、髓样分化因子88（Myd88）及蛋白激酶C（PKC）]的表达。

结果

与正常对照组相比，LPS组小鼠脑组织的海马区神经元排列稀疏紊乱，大量神经元固缩，核缩小；脑组织中TNF-α、IL-1β、IL-6、NO含量均显著增加，IL-10含量显著降低，iNOS、COX-2、Myd88、PKC蛋白相对表达量均显著升高（

＜0.05）。与LPS组相比，TFB低、中、高剂量组小鼠脑组织神经元病理变化明显改善，脑组织中上述指标变化均显著逆转（

＜0.05）。

结论

TFB具有抑制神经炎症的作用，其作用机制可能与下调炎症通路相关蛋白iNOS、COX-2、Myd88和PKC表达，减少炎症因子释放有关。

Abstract

OBJECTIVE

To investigate

the effect and mechanism of total flavonoids of

Bidens pilosa

L. （TFB） on lipopolysaccharide （LPS）-induced neuroinflammation in mice.

METHODS

Fifty C57BL/6 mice were randomly divided into normal control group， LPS group and TFB low-dose， medium-dose and high-dose groups， with 10 mice in each group. TFB low-dose， medium-dose and high-dose groups were given TFB solution intragastrically at 60， 120 and 240 mg/kg， and the normal control group and LPS group were given corresponding volume of normal saline， once a day， for consecutive 21 d. From the 15th day of administration， except for the normal control group， other groups were given LPS （400 μg/kg） intraperitoneally for 7 consecutive days to establish neuroinflammatory model. Brain tissues were taken under anesthesia 4 h after the final administration. The morphological changes of neuronal cells in mice were observed； the contents of nitric oxide （NO）， tumor necrosis factor α （TNF-α）， interleukin-1β （IL-1β）， IL-6 and IL-10 were measured， and the expressions of inflammatory pathway-related proteins [inducible NO synthase （iNOS）， cyclooxygenase-2 （COX-2）， myeloid differentiation factor 88 （Myd88） and protein kinase C （PKC）] were measured in the brain tissues of mice.

RESULTS

Compared with the normal control group， the neuronal arrangement in the hippocampal region of the brain tissue of mice in the LPS group was sparsely disorganized， with a large number of neuronal fixations and shrunken nuclei； the contents of TNF-α， IL-1β， IL-6 and NO in the brain tissue were significantly increased， the contents of IL-10 were significantly decreased， and the relative expressions of iNOS， COX-2， Myd88 and PKC proteins were significantly increased （

＜0.05）. Compared with the LPS group， the neuronal pathological changes in the brain tissue of mice in the TFB low-dose， medium-dose and high-dose groups were significantly improved， and the changes of the above indices in the brain tissue we

re significantly reversed （

＜0.05）.

CONCLUSIONS

TFB has an inhibitory effect on neuroinflammation， and its mechanism of action may be related to down-regulation of the expressions of inflammatory pathway-related proteins iNOS， COX-2， Myd88 and PKC， and reduction of inflammatory factors release.

关键词

Keywords

references

SINGHAL G ， JAEHNE E J ， CORRIGAN F ， et al . Inflammasomes in neuroinflammation and changes in brain function：a focused review [J ] . Front Neurosci ， 2014 ， 8 ： 315 .

赵铭，李冬，朱迅，等 . 小胶质细胞在中枢神经系统疾病中作用的研究进展 [J ] . 现代免疫学， 2018 ， 38 （ 5 ）： 425 - 429 .

SHABAB T ， KHANABDALI R ， MOGHADAMTOUSI S Z ， et al . Neuroinflammation pathways：a general review [J ] . Int J Neurosci ， 2017 ， 127 （ 7 ）： 624 - 633 .

曹园，瞿慧，姚毅，等 . 鬼针草化学成分研究 [J ] . 中草药， 2013 ， 44 （ 24 ）： 3435 - 3439 .

潘海敏，方慧华，严士海 . 鬼针草总黄酮对体外血小板聚集的影响 [J ] . 医药导报， 2011 ， 30 （ 10 ）： 1273 - 1274 .

张媛媛，彭磊，徐涛，等 . 鬼针草总黄酮对高脂血症大鼠脂质代谢的影响 [J ] . 安徽医药， 2012 ， 16 （ 9 ）： 1247 - 1249 .

庞晓军，卢琳琳，黎东旺，等 . 鬼针草总黄酮对HepG2细胞胰岛素抵抗的影响 [J ] . 中国药房， 2022 ， 33 （ 8 ）： 968 - 974 .

程新燕 . 鬼针草总黄酮对D-GalN致急性肝损伤小鼠的保护作用 [J ] . 中国实验方剂学杂志， 2013 ， 19 （ 14 ）： 268 - 271 .

曾菊，程斌，程肖蕊，等 . 基于LPS诱导小鼠炎症模型的LW-AFC抗炎作用研究 [J ] . 神经药理学报， 2018 ， 8 （ 2 ）： 44 .

JAYARAJ R L ， AZIMULLAH S ， BEIRAM R ， et al . Neuroinflammation：friend and foe for ischemic stroke [J ] . J Neuroinflammation ， 2019 ， 16 （ 1 ）： 142 .

ORIHUELA R ， MCPHERSON C A ， HARRY G J . Microglial M1/M2 polarization and metabolic states [J ] . Br J Pharmacol ， 2016 ， 173 （ 4 ）： 649 - 665 .

WEN X J ， XIAO L J ， ZHONG Z Y ， et al . Astaxanthin acts via LRP-1 to inhibit inflammation and reverse lipopolysaccharide-induced M1/M2 polarization of microglial cells [J ] . Oncotarget ， 2017 ， 8 （ 41 ）： 69370 - 69385 .

阮洪生，牟晋珠 . 表儿茶素对脂多糖诱导RAW 264 .7细胞分泌炎症因子的影响[J ] . 中国实验方剂学杂志，2017， 23 （ 4 ）： 159 - 163 .

刘思远，李志鹏，彭召云，等 . 花青素对肺癌细胞相关炎性因子信号转导通路作用的研究进展 [J ] . 中国实验方剂学杂志， 2017 ， 23 （ 14 ）： 219 - 225 .

AKBAR M ， ESSA M M ， DARADKEH G ， et al . Mitochondrial dysfunction and cell death in neurodegenerative diseases through nitroxidative stress [J ] . Brain Res ， 2016 ， 1637 ： 34 - 55 .

KIM Y C ， LEE S E ， KIM S K ， et al . Toll-like receptor mediated inflammation requires FASN-dependent MYD88 palmitoylation [J ] . Nat Chem Biol ， 2019 ， 15 （ 9 ）： 907 - 916 .

PATRA S ， MUTHURAMAN M S ， MEENU M ， et al . Anti-inflammatory effects of royal poinciana through inhibition of toll-like receptor 4 signaling pathway [J ] . Int Immunopharmacol ， 2016 ， 34 ： 199 - 211 .

ZHAO L ， LI M Y ， SUN K C ， et al . Hippophae rham-noides polysaccharides protect IPEC-J2 cells from LPS-induced inflammation，apoptosis and barrier dysfunction in vitro via inhibiting TLR4/NF-κB signaling pathway [J ] . Int J Biol Macromol ， 2020 ， 155 ： 1202 - 1215 .

WEN J ， RIBEIRO R ， ZHANG Y M . Specific PKC isoforms regulate LPS-stimulated iNOS induction in murine microglial cells [J ] . J Neuroinflammation ， 2011 ， 8 ： 38 .

LIU Q ， ZHANG Y L ， LIU S ， et al . Cathepsin C promotes microglia M1 polarization and aggravates neuroinflam-mation via activation of Ca 2+ -dependent PKC/p38MAPK/NF-κB pathway [J ] . J Neuroinflammation ， 2019 ， 16 （ 1 ）： 10 .

Views

144

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Clinical observation of Huangkui capsule combined with cyclophosphamide and prednisone in the treatment of IgA nephropathy with renal insufficiency

Clinical observation of enteral immune microecological nutrition combined with ω-3 fish oil fat emulsion for postoperative hepatocellular carcinoma resection

Mechanism of Wumen Zhiqiao gancao decoction inhibiting pathological angiogenesis in degenerative intervertebral discs by regulating HIF-1α/VEGF/Ang signal axis

Improvement effect and mechanism of petroleum ether extract of Saposhnikovia divaricata on rheumatoid arthritis rats by regulating neutrophil extracellular traps

Efficacy observation of Baogong zhixue granules combined with medroxyprogesterone acetate in the treatment of abnormal uterine bleeding （yin deficiency and blood heat syndrome） during perimenopause

Related Author

JIANG Yan

ZHANG Ming

LIU Guoxin

ZHAO Chenyang

ZHANG Ming

YAN Huaying

XU Dongping

WANG Jingjing

Related Institution

Dept. of Pharmacy， the Second Affiliated Hospital of Dalian Medical University

Dept. of Gastrointestinal and Anorectal Surgery， Tianjin Third Central Hospital

Dept. of Nutrition， Tianjin Third Central Hospital

Dept. of Orthopedics and Traumatology， Qufu Hospital of Traditional Chinese Medicine， Shandong Qufu

Orthopaedic Traumatology Institute， Suzhou Academy of Wumen Chinese Medicine

⁰

Improvement effects and mechanism of total flavonoids of Bidens pilosa on lipopolysaccharide-induced neuroinflammation in mice

DOI：10.6039/j.issn.1001-0408.2023.14.11

摘要

Abstract

关键词

Keywords

references