Simultaneous determination of 8 components in Jinqi jiangtang tablets by ion mobility mass spectrometry method

LI Yingcai; HUANG Hecheng; YANG Ye

doi:10.6039/j.issn.1001-0408.2023.11.13

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Simultaneous determination of 8 components in Jinqi jiangtang tablets by ion mobility mass spectrometry method

更新时间：2025-09-27

- Simultaneous determination of 8 components in Jinqi jiangtang tablets by ion mobility mass spectrometry method
- ZHONGGUO YAOFANG Vol. 34, Issue 11, Pages: 1354-1357(2023)
- 作者机构：
  
  1.昆明市食品药品检验所，昆明　650032
  2.云南孚尔质量检验检测有限公司，昆明　650214
  3.昆明理工大学生命科学与技术学院，昆明　650500
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2023.11.13
  CLC： R917
- Received：16 January 2023，
  
  Revised：2023-04-23，
  
  Accepted：23 April 2023，
  
  Published：15 June 2023
- 稿件说明：
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李应才,黄合琤,杨野.离子淌度质谱法同时测定金芪降糖片中8种成分含量[J].中国药房,2023,34(11):1354-1357.

LI Yingcai,HUANG Hecheng,YANG Ye.Simultaneous determination of 8 components in Jinqi jiangtang tablets by ion mobility mass spectrometry method[J].ZHONGGUO YAOFANG,2023,34(11):1354-1357.
李应才,黄合琤,杨野.离子淌度质谱法同时测定金芪降糖片中8种成分含量[J].中国药房,2023,34(11):1354-1357. DOI： 10.6039/j.issn.1001-0408.2023.11.13.

LI Yingcai,HUANG Hecheng,YANG Ye.Simultaneous determination of 8 components in Jinqi jiangtang tablets by ion mobility mass spectrometry method[J].ZHONGGUO YAOFANG,2023,34(11):1354-1357. DOI： 10.6039/j.issn.1001-0408.2023.11.13.

摘要

目的

建立离子淌度质谱法同时测定金芪降糖片中表小檗碱、小檗碱、黄连碱、巴马汀、毛蕊异黄酮葡萄糖苷、3，5-二-

-咖啡酰奎宁酸、4，5-二-

-咖啡酰奎宁酸、绿原酸的含量。

方法

采用离子淌度质谱法进行检测。色谱柱为Waters ACQUITY UPLC HSS T3（2.1 mm×50 mm，1.8 μm），流动相为0.1%甲酸溶液-乙腈（梯度洗脱），流速为0.3 mL/min，柱温为40 ℃，进样量为5 μL。采用电喷雾离子源，在正、负离子模式下进行扫描检测，并根据各成分峰响应设置离子淌度参数，对金芪降糖片中8种成分进行含量测定。

结果

8种成分在各自范围内线性关系良好（

≥0.999）；精密度、重复性、稳定性（24 h）试验的RSD均未超过4.0%；平均加样回收率为 94.6%～101.2%，RSD为2.6%～3.9%（

＝9）。3批金芪降糖片中上述8种成分的含量分别为3.060～3.545、24.50～26.74、2.795～4.149、1.437～2.501、0.204～0.242、0.950～1.281、2.272～2.828、7.314～7.960 mg/g。

结论

所建方法灵敏度高、重复性好，可为该制剂的质量控制提供参考。

Abstract

OBJECTIVE

To establish the ion mobility mass spectrometry method for simultaneous determination of epiberberine， berberine， coptisine， palmatine， calycosin-7-glucoside， 3，5-

-dicaffeoylquinic acid， 4，5-

-dicaffeoylquinic acid and chlorogenic acid in Jinqi jiangtang table

ts.

METHODS

Ion mobility mass spectrometry method was used. The determination was performed on Waters ACQUITY UPLC HSS T3 （2.1 mm×50 mm， 1.8 μm） with mobile phase consisted of 0.1% formic acid solution-acetonitrile （gradient elution） at the flow rate of 0.3 mL/min. The column temperature was 40 ℃， and the injection volume was 5 μL. The contents of 8 components in Jinqi jiangtang tablets were determined by scanning detection under positive and negative ion modes with an electric spray ion source， and setting ion mobility mass parameters according to the peak response of each component.

RESULTS

The results showed that the linear relationship of the eight components was good within their respective ranges （

≥0.999）； RSDs of precision， repeatability and stability （24 h） tests were not more than 4.0%； average recoveries were 94.6%-101.2% ， RSDs were 2.6%-3.9% （

＝9）. The contents of the above eight components in three batches of Jinqi jiangtang tablets were 3.060-3.545， 24.50-26.74， 2.795-4.149， 1.437-2.501， 0.204-0.242， 0.950-1.281， 2.272-2.828， 7.314-7.960 mg/g， respectively.

CONCLUSIONS

The established method has high sensitivity and good reproducibility， and can provide reference for the quality control of the preparation.

关键词

Keywords

references

国家药典委员会 . 中华人民共和国药典：一部 [S ] . 2020年版. 北京：中国医药科技出版社， 2020 ： 60 - 65 .

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马恒，陈靓，陈勇 . HPLC同时测定金芪降糖片中小檗碱及绿原酸的含量 [J ] . 湖北大学学报（自然科学版）， 2016 ， 38 （ 4 ）： 294 - 297 .

于静，邓雁如，陈奇，等 . HPLC测定金银花及金芪降糖片中6种成分的含量 [J ] . 中国实验方剂学杂志， 2011 ， 17 （ 19 ）： 57 - 60 .

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连显会，王春，孟宪双，等 . 超高效液相色谱-差分离子淌度质谱法测定化妆品中10种卡因类禁用组分 [J ] . 分析化学， 2019 ， 47 （ 5 ）： 756 - 764 .

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