Study on quality control method of Qiguiling mixture

YU Xiaotao; WANG Bo; WANG Jia; CHEN Kejia; WANG Rui

doi:10.6039/j.issn.1001-0408.2023.10.13

您当前的位置：

首页 >

文章列表页 >

Study on quality control method of Qiguiling mixture

更新时间：2025-09-27

- Study on quality control method of Qiguiling mixture
- ZHONGGUO YAOFANG Vol. 34, Issue 10, Pages: 1223-1227(2023)
- 作者机构：
  
  漯河市中心医院药学部/河南省中药制剂现代化技术研发与临床应用工程研究中心，河南漯河　462000
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2023.10.13
  CLC： R917
- Received：02 December 2022，
  
  Revised：2023-04-24，
  
  Accepted：27 April 2023，
  
  Published：30 May 2023
- 稿件说明：
移动端阅览
于晓涛,王博,王佳,等.芪桂苓合剂质量控制方法研究[J].中国药房,2023,34(10):1223-1227.

YU Xiaotao,WANG Bo,WANG Jia,et al.Study on quality control method of Qiguiling mixture[J].ZHONGGUO YAOFANG,2023,34(10):1223-1227.
于晓涛,王博,王佳,等.芪桂苓合剂质量控制方法研究[J].中国药房,2023,34(10):1223-1227. DOI： 10.6039/j.issn.1001-0408.2023.10.13.

YU Xiaotao,WANG Bo,WANG Jia,et al.Study on quality control method of Qiguiling mixture[J].ZHONGGUO YAOFANG,2023,34(10):1223-1227. DOI： 10.6039/j.issn.1001-0408.2023.10.13.

摘要

目的

建立芪桂苓合剂的指纹图谱和毛蕊异黄酮葡萄糖苷等4种活性成分含量的测定方法，以控制芪桂苓合剂的质量。

方法

采用高效液相色谱（HPLC）法建立12批芪桂苓合剂的指纹图谱；采用SPSS 25.0软件进行聚类分析、主成分分析，采用SIMCA 14.1软件进行正交偏最小二乘法-判别分析，并以变量重要性投影（VIP）值大于1.0为标准，筛选差异性成分。以橙皮苷为内参物，采用一测多评法计算毛蕊异黄酮葡萄糖苷、甘草苷、甘草酸含量，并与外标法的测定结果进行比较。

结果

12批样品指纹图谱中共确认了17个共有峰，相似度均大于0.940；共指认出4个共有峰，分别为毛蕊异黄酮葡萄糖苷（6号峰）、甘草苷（8号峰）、橙皮苷（12号峰）和甘草酸（17号峰）。12批样品可聚类分为2类，其中S4、S7～S9、S11～S12聚为一类，其他聚为一类；6个主成分的累计方差贡献率为85.840%；15、14、4、8（甘草苷）、9号峰的VIP值均大于1.0。毛蕊异黄酮葡萄糖苷、甘草苷、甘草酸含量的一测多评法结果与外标法所得结果的相对误差均小于5%（

＝3）。

结论

本研究建立的HPLC指纹图谱和含量测定方法，可用于芪桂苓合剂的质量控制；甘草苷等5种成分是其差异性成分。

Abstract

OBJECTIVE

To establish the fingerprint of Qiguiling mixture and the method for the content determination of 4 kinds of active components such as calycosin-7-glucoside， so as to control the quality of Qiguiling mixture.

METHODS

The fingerprints of 12 batches of Qiguiling mixture were established by HPLC. SPSS 25.0 software was used for cluster analysis and principal component analysis， and SIMCA 14.1 software was used for orthogonal partial least squares-discriminant analysis. The variable importance in projection （VIP） value greater than 1.0 was used as the index to screen the differential components. The contents of calycosin-7-glucoside， glycyrrhizin and glycyrrhizic acid were calculated by the quantitative analysis of multi-components by single marker （QAMS） with hesperidin as the internal reference， and the results were compared with external standard method.

RESULTS

In the fingerprints of 12 batches of samples， 17 common peaks were identified， and the similarities were more than 0.940. A total of 4 common peaks were identified， which were calycosin-7-glucoside （peak 6）， glycyrrhizin （peak 8）， hesperidin （peak 12）， and glycyrrhizic acid （peak 17）. The 12 batches of samples could be clustered into two categories， S4， S7-S9 and S11-S12 were clustered into one category， and the other batches of samples were clustered into one category. The cumulative variance contribution rate of the six principal components was 85.840%， and VIP values of peaks 15， 14， 4， 8 （glycyrrhizin） and 9 were all greater than 1.0. The relative error between the results of QAMS and external standard method was less than 5% （

＝3） for the contents of calycosin-7-glucoside， glycyrrhizin and glycyrrhizic acid.

CONCLUSIONS

Established HPLC fingerprint and content determination method in this study can be used for quality control of Qigiling mixture. Five components such as glycyrrhizin are the differential components.

关键词

Keywords

references

杨忠杰，叶琳，陈朗，等 . 芪桂苓合剂治疗新型冠状病毒肺炎 [J ] . 中医学报， 2020 ， 35 （ 6 ）： 1151 - 1153，1176 .

XU X Y ， LI F ， ZHANG X ， et al . In vitro synergistic anti-oxidant activity and identification of antioxidant components from Astragalus membranaceus and Paeonia lactiflora [J ] . PLoS One ， 2014 ， 9 （ 5 ）： e96780 .

李健，韩林，马玉芳，等 . 黄芪3种成分对Chang Liver细胞氧化应激的抑制作用 [J ] . 中国中药杂志， 2015 ， 40 （ 2 ）： 318 - 323 .

焦士蓉，黄承钰 . 柑橘属类黄酮生物活性的研究进展 [J ] . 西华大学学报（自然科学版）， 2008 ， 27 （ 1 ）： 32 - 35，103 .

KIM D H ， SONG M J ， BAE E A ， et al . Inhibitory effect of herbal medicines on rotavirus infectivity [J ] . Biol Pharm Bull ， 2000 ， 23 （ 3 ）： 356 - 358 .

葛孚晶，曾晨鸣，严芳洁，等 . 甘草酸：一种治疗新型冠状肺炎的潜在药物 [J ] . 药学学报， 2021 ， 56 （ 5 ）： 1211 - 1216 .

KUANG Y ， LI B ， FAN J ， et al . Antitussive and expectorant activities of licorice and its major compounds [J ] . Bioorg Med Chem ， 2018 ， 26 （ 1 ）： 278 ．

王博，于晓涛，刘玉萍，等 . 蒲蓝利咽合剂的指纹图谱建立及含量测定 [J ] . 中国药房， 2022 ， 33 （ 22 ）： 2753 - 2757 .

胡瑞雪，梁元昊，徐文丽，等 . 一测多评法在中药中的应用及研究进展 [J ] . 药物分析杂志， 2019 ， 39 （ 11 ）： 1968 - 1979 .

杨莎，宋忠兴，唐志书，等 . 一测多评法同时测定阿娜尔妇洁液中7种成分 [J ] . 中草药， 2021 ， 52 （ 12 ）： 3560 - 3567 .

赵峰，高太祥，石莉尧，等 . 越婢加术汤的HPLC指纹图谱建立、含量测定及化学模式识别分析 [J ] . 中国药房， 2021 ， 32 （ 22 ）： 2724 - 2730 .

黄娜娜，李俊，饶高雄，等 . 基于HPLC指纹图谱结合多种化学模式识别评价生脉饮（党参方）质量 [J ] . 中国医院药学杂志， 2021 ， 41 （ 23 ）： 2433 - 2442 .

王智民，钱忠直，张启伟，等 . 一测多评法建立的技术指南 [J ] . 中国中药杂志， 2011 ， 36 （ 6 ）： 657 - 658 .

宝鲁尔，王徽，吴杰斯，等 . 一测多评法同时测定蒙药扎冲十三味丸中8种成分 [J ] . 中草药， 2021 ， 52 （ 11 ）： 3249 - 3256 .

Views

139

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Establishment of quantitative analysis of multi-components by single-marker method for content determination of flavonoids and phenolic acids in Perilla frutescens leaves

Study on quality control method of the roots and rhizoma of Toricellia angulata

Identification of components， fingerprint and content determination of the effective parts of Dracocephalum moldavica

Evaluation of the quality of Alpinia katsumadai from different habitats based on HPLC fingerprint and determination of active ingredient content

Quality evaluation of Xintong granules based on HPLC fingerprint and quantitative analysis of multi-components by single-marker method

Related Author

LI Danyang

DONG Chao

ZHENG Yunfeng

YAN Hui

ZHANG Li

NIE Yushan

MA Xue

LU Yuan

Related Institution

School of Pharmacy， Nanjing University of Chinese Medicine

Dept. of Pharmacy， the First Affiliated Hospital with Nanjing Medical University

Dept. of Pharmacy， Jiangsu Province Hospital of Chinese Medicine

Guizhou Provincial Key Laboratory of Pharmaceutics， Guizhou Medical University

School of Pharmacy， Guizhou Medical University

⁰