Effects of <italic style="font-style: italic">Cannabis sativa</italic> oil on the symptom improvement and intestinal flora in UC model rats

GONG Mengjuan; GAO Xinyue; JIAN Xiaomin; ZOU Zhongjie

doi:10.6039/j.issn.1001-0408.2023.06.10

您当前的位置：

首页 >

文章列表页 >

Effects of Cannabis sativa oil on the symptom improvement and intestinal flora in UC model rats

更新时间：2025-09-27

- Effects of Cannabis sativa oil on the symptom improvement and intestinal flora in UC model rats
- ZHONGGUO YAOFANG Vol. 34, Issue 6, Pages: 693-698(2023)
- 作者机构：
  
  广东药科大学中药学院，广州　510006
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2023.06.10
  CLC： R965
- Received：30 September 2022，
  
  Revised：2023-02-16，
  
  Published：30 March 2023
- 稿件说明：
移动端阅览
龚梦鹃,高心悦,简晓敏,等.火麻仁油改善UC模型大鼠的症状及对肠道菌群的影响[J].中国药房,2023,34(06):693-698.

GONG Mengjuan,GAO Xinyue,JIAN Xiaomin,et al.Effects of Cannabis sativa oil on the symptom improvement and intestinal flora in UC model rats[J].ZHONGGUO YAOFANG,2023,34(06):693-698.
龚梦鹃,高心悦,简晓敏,等.火麻仁油改善UC模型大鼠的症状及对肠道菌群的影响[J].中国药房,2023,34(06):693-698. DOI： 10.6039/j.issn.1001-0408.2023.06.10.

GONG Mengjuan,GAO Xinyue,JIAN Xiaomin,et al.Effects of Cannabis sativa oil on the symptom improvement and intestinal flora in UC model rats[J].ZHONGGUO YAOFANG,2023,34(06):693-698. DOI： 10.6039/j.issn.1001-0408.2023.06.10.

摘要

目的

研究火麻仁油对右旋葡聚糖硫酸钠（DSS）诱导的溃疡性结肠炎（UC）模型大鼠症状的改善作用，并探讨其对大鼠肠道菌群的影响。

方法

将40只SD大鼠随机分为对照组、模型组、火麻仁油组（1 g/kg）和柳氮磺胺吡啶组（阳性对照，300 mg/kg），每组10只。对照组和模型组大鼠灌胃0.5%聚山梨酯80，火麻仁油组和柳氮磺胺吡啶组大鼠灌胃相应药液，每天1次，连续10 d。从第4天开始，模型组、火麻仁油组和柳氮磺胺吡啶组大鼠连续7 d饮用4%DSS溶液以建立UC模型。观察大鼠体质量、疾病活动指数（DAI）评分、结肠长度、结肠质量、结肠单位长度质量、结肠组织病理学变化，检测其血清肿瘤坏死因子α（TNF-α）、白细胞介素6（IL-6）、IL-10含量，通过高通量测序法检测各组大鼠肠道菌群的变化。

结果

与对照组比较，模型组大鼠的体质量显著降低，结肠长度显著缩短，DAI评分、结肠质量、结肠单位长度质量和血清TNF-α、IL-6含量均显著增加，IL-10含量显著降低（

＜0.05）；结肠组织上皮层发生脱落，炎症细胞浸润且入侵黏膜下层，肠腺紊乱。与模型组比较，火麻仁油组和柳氮磺胺吡啶组大鼠上述指标均显著逆转（

＜0.05），且相关症状均有明显改善。菌群测序结果显示，模型组大鼠的ACE指数和Chao1指数均较对照组显著降低（

＜0.05），火麻仁油组大鼠的Chao1指数较模型组显著升高（

＜0.05）。与对照组比较，模型组有41个菌属发生改变；与模型组比较，火麻仁油能使上述41个菌属中的3个菌属回调至正常状态，包括杜波西菌属、紫杆菌属和别样棒菌属。

结论

火麻仁油能通过调节肠道菌群多样性，增加有益菌和减少有害菌来改善UC模型大鼠症状。

Abstract

OBJECTIVE

To study the improvement effects of

Cannabis sativa

oil on the symptoms in dextran sodium sulfate （DSS）-induced ulcerative colitis （UC） model rats， and to investigate its effects on intestinal flora of rats.

METHODS

Forty SD rats were randomly divided into control group， model group，

C. sativa

oil group （1 g/kg） and sulfasalazine group （positive control， 300 mg/

kg）， with 10 rats in each group. The rats in control group and model group were given 0.5% polysorbate 80 by gavage， and the rats in

C. sativa

oil group and sulfasalazine group were given corresponding drug solution by gavage once a day for 10 days. From the 4th day， rats in model group，

C. sativa

oil group and sulfasalazine group were given 4% DSS solution for 7 consecutive days to establish UC model. The body weight， disease activity index （DAI） score， colon length， colon weight， weight per unit length of colon， the pathological changes of colon tissue， and the contents of tumour necrosis factor-α （TNF-α）， interleukin-6 （IL-6） and IL-10 in serum of rats were determined. The changes of intestinal flora in rats were detected by high-throughput sequencing.

RESULTS

Compared with control group， the body weight and the length of colon were decreased significantly in model group， while DAI score， the weight of colon， weight per unit length of colon， serum contents of TNF-α and IL-6 were increased significantly， and the content of IL-10 was decreased significantly （

＜0.05）； epithelial layer of colon tissue fell off， inflammatory cells infiltrated and invaded the submucosa， and intestinal glands were disordered. Compared with model group， above indexes of

C. sativa

oil group and sulfasalazine group were reversed significantly （

＜0.05）， and related symptoms were improved significantly. The result of flora sequencing showed that ACE index and Chao1 index of model group were decreased significantly， compared with control group （

＜0.05）； while Chao1 index of

C. sativa

oil group was increased significantly， compared with model group （

＜0.05）. Compared with control group， 41 genera of bacteria in the model group changed； compared with model group，

C. sativa

oil could return 3 of the 41 genera to normal state， including

Dubosilla

，

Porphyrobacter

and

Allobaculum

CONCLUSIONS

C. sativa

oil can improve the symptoms of UC model rats by regulating the diversity of intestinal flora， increasing beneficial bacteria and decreasing pathogenic bacteria.

关键词

Keywords

references

李寒冰，吴宿慧，李根林，等 . 火麻仁油对便秘大鼠肠道微生态的改善作用 [J ] . 中华中医药学刊， 2018 ， 36 （ 8 ）： 1878 - 1881 .

李根林，郭晨阳，吴宿慧，等 . 火麻仁油对D-半乳糖致衰老小鼠肠道功能调节作用研究 [J ] . 中草药， 2020 ， 51 （ 13 ）： 3509 - 3517 .

任汉阳，张瑜，刘红雨，等 . 火麻仁油对便秘模型小鼠抗氧化作用的实验研究 [J ] . 中国医药学报， 2004 ， 19 （ 2 ）： 123 - 124 .

HUANG Y C ， ZHENG Y H ， YANG F M ， et al . Lycium barbarum glycopeptide prevents the development and progression of acute colitis by regulating the composition and diversity of the gut microbiota in mice [J ] . Front Cell Infect Microbiol ， 2022 ， 12 ： 921075 .

WU J W ， WEI Z H ， CHENG P ， et al . Rhein modulates host purine metabolism in intestine through gut microbiota and ameliorates experimental colitis [J ] . Therano-stics ， 2020 ， 10 （ 23 ）： 10665 - 10679 .

ZHU L ， GU P Q ， SHEN H . Protective effects of berberine hydrochloride on DSS-induced ulcerative colitis in rats [J ] . Int Immunopharmacol ， 2019 ， 68 ： 242 - 251 .

刘思雨，郎晓猛，刘建平，等 . 基于肠道菌群变化探讨健脾泄浊解毒方预防缓解期溃疡性结肠炎大鼠复发的实验研究 [J ] . 河北中医， 2022 ， 44 （ 9 ）： 1507 - 1511 .

LIU F L ， YAO Y F ， LU Z H ， et al . 5-hydroxy-4-methoxycanthin-6-one alleviates dextran sodium sulfate-induced colitis in rats via regulation of metabolic profiling and suppression of NF-κB/p65 signaling pathway [J ] . Phytomedicine ， 2021 ， 82 ： 153438 .

CHASSAING B ， AITKEN J D ， MALLESHAPPA M ， et al . Dextran sulfate sodium（DSS）-induced colitis in mice [J ] . Curr Protoc Immunol ， 2014 ，104：15.25. 1 - 15 .25.14.

QIU X X ， MACCHIETTO M G ， LIU X T ， et al . Identification of gut microbiota and microbial metabolites regulated by an antimicrobial peptide lipocalin 2 in high fat diet-induced obesity [J ] . Int J Obes（Lond）， 2021 ， 45 （ 1 ）： 143 - 154 .

艾静，王承党 . 丁酸盐与炎症性肠病的研究进展 [J ] . 胃肠病学， 2014 ， 19 （ 1 ）： 47 - 49 .

LI Y Y ， YAN H M ， ZHANG Y W ， et al . Alterations of the gut microbiome composition and lipid metabolic profile in radiation enteritis [J ] . Front Cell Infect Microbiol ， 2020 ， 10 ： 541178 .

LIU T H ， TAO W C ， LIANG Q E ， et al . Gut microbiota-related evidence provides new insights into the association between activating transcription factor 4 and development of salt-induced hypertension in mice [J ] . Front Cell Dev Biol ， 2020 ， 8 ： 585995 .

LI D P ， CUI M ， TAN F ， et al . High red meat intake exacerbates dextran sulfate-induced colitis by altering gut microbiota in mice [J ] . Front Nutr ， 2021 ， 8 ： 646819 .

WAN F ， HAN H ， ZHONG R Q ， et al . Dihydroquercetin supplement alleviates colonic inflammation potentially through improved gut microbiota community in mice [J ] . Food Funct ， 2021 ， 12 （ 22 ）： 11420 - 11434 .

张颖，杨悦，韦庆慧，等 . 鞘氨醇单胞菌的特性及应用研究进展 [J ] . 化学与生物工程， 2021 ， 38 （ 3 ）： 6 - 13 .

FATAHI-BAFGHI M . Characterization of the Rothia spp. and their role in human clinical infections [J ] . Infect Genet Evol ， 2021 ， 93 ： 104877 .

KUMAR M ， GARAND M ， AL KHODOR S . Integrating omics for a better understanding of inflammatory bowel disease：a step towards personalized medicine [J ] . J Transl Med ， 2019 ， 17 （ 1 ）： 419 .

Views

131

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Mechanism of astragalin in allevating ulcerative colitis in mice through modulation of the intestinal flora

Study on metabolites derived from Zhideke granules in rats in vivo

Effects of Yiru tiaojing granules on the pharmacokinetics of olanzapine in rats

Improvement effects of Shangke xiaoyan hydrogel plaster on osteoarthritis model rats

Study on protective effects of twin drugs of tetramethylpyrazine-scutellarein on cerebral ischemia-reperfusion injury model rats and its mechanism

Related Author

HUANG Jing

LIAO Yanhua

MO Xinying

YANG Yuting

JIANG Weizhe

ZHENG Piaoxue

CHEN Huihua

HUANG Chunyan

Related Institution

School of Pharmacy， Guangxi Medical University

Dept. of Pharmacy， the Affiliated Hospital of Youjiang Medical University for Nationalities

Dept. of Pharmacy， Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine

Qinzhou Inspection and Testing Center

Guangxi Zhuang Yao Medicine Engineering Technology Research Center

⁰

Effects of Cannabis sativa oil on the symptom improvement and intestinal flora in UC model rats

DOI：10.6039/j.issn.1001-0408.2023.06.10

摘要

Abstract

关键词

Keywords

references