Comparison of characteristic chromatogram and the contents of multiple indicator components of <italic style="font-style: italic">Morus alba</italic> decoction powder and decoction at different decoction time

JIANG Jinjin; WU Lei; ZHU Yufeng; SUN Si; QIU Mingming; LU Chao

doi:10.6039/j.issn.1001-0408.2023.05.10

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Comparison of characteristic chromatogram and the contents of multiple indicator components of Morus alba decoction powder and decoction at different decoction time

更新时间：2025-09-27

- Comparison of characteristic chromatogram and the contents of multiple indicator components of Morus alba decoction powder and decoction at different decoction time
- ZHONGGUO YAOFANG Vol. 34, Issue 5, Pages: 565-568(2023)
- 作者机构：
  
  1.南京中医药大学附属医院/江苏省中医院药学部，南京　210029
  2.南京中医药大学第一临床医学院，南京　210029
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2023.05.10
  CLC： R917
- Received：29 September 2022，
  
  Revised：2023-01-05，
  
  Published：15 March 2023
- 稿件说明：
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蒋近近,吴磊,朱育凤,等.不同煎煮时间桑白皮汤煮散与汤剂的特征图谱及多指标成分含量比较[J].中国药房,2023,34(05):565-568.

JIANG Jinjin,WU Lei,ZHU Yufeng,et al.Comparison of characteristic chromatogram and the contents of multiple indicator components of Morus alba decoction powder and decoction at different decoction time[J].ZHONGGUO YAOFANG,2023,34(05):565-568.
蒋近近,吴磊,朱育凤,等.不同煎煮时间桑白皮汤煮散与汤剂的特征图谱及多指标成分含量比较[J].中国药房,2023,34(05):565-568. DOI： 10.6039/j.issn.1001-0408.2023.05.10.

JIANG Jinjin,WU Lei,ZHU Yufeng,et al.Comparison of characteristic chromatogram and the contents of multiple indicator components of Morus alba decoction powder and decoction at different decoction time[J].ZHONGGUO YAOFANG,2023,34(05):565-568. DOI： 10.6039/j.issn.1001-0408.2023.05.10.

摘要

目的

比较不同煎煮时间桑白皮汤煮散与汤剂（后文简称为“煮散”和“汤剂”）物质基准的特征图谱与多指标成分含量变化，为桑白皮汤的制剂开发提供实验基础。

方法

以不同煎煮时间的煮散与汤剂样品为研究对象。利用《中药色谱指纹图谱相似度评价系统（2012版）》软件建立2种样品的高效液相色谱（HPLC）特征图谱，并进行相似度评价；采用HPLC法测定煮散与汤剂样品中桑皮苷A、栀子苷、小檗碱、黄芩苷、槲皮素和木犀草素含量，以各指标成分含量及总含量变化为评价指标比较二者煎煮过程的差异。

结果

不同煎煮时间煮散和汤剂特征图谱的相似度分别为0.943～1.000、0.975～0.998。煮散中6种指标成分的溶出速率较快且含量较高；煎煮20 min时，煮散中各指标成分含量分别为汤剂煎煮50 min时的1.1～1.5倍，煮散中各指标成分的总含量为汤剂的1.2倍。

结论

与汤剂相比，桑白皮汤煮散具有缩短煎煮时间、节约中药资源的优势。本研究结果为“遵古”开发本方剂奠定了基础。

Abstract

OBJECTIVE

To compare characteristic chromatogram and the contents of multiple indicator components of

Morus alba

decoction powder and decoction at different decoction time， and to provide experimental basis for the development of

M. alba

decoction.

METHODS

Taking decoction powder and decoction at different decoction time as subject， HPLC characteristic chromatogram of 2 kinds of samples were established with

Similarity Evaluation Software System of TCM Chromatographic Fingerprint

（2012 version）， and similarity evaluation was performed. The contents of mulberroside A， geniposide， berberine， baicalin， quercetin and luteolin in decoction powder and decoction were determined by HPLC. The contents of each indicator component and the change of total content were as the evaluation indexes to compare the difference between the two substances during decoction.

RESULTS

The similarities of characteristic chromatogram of the two substances ranged from 0.943 to 1.000 and 0.975 to 0.998 at different decoction time， respectively. Six indicator components of the decoction powder dissolved faster and had higher contents. The contents of each indicator component in the decoction powder when decocting at 20 minutes was 1.1-1.5 times of the decoction when decocting at 50 min， and the total content in the decoction powder was 1.2 times of the decoction.

CONCLUSIONS

Compared with decoction，

M. alba

decoction powder has the advantages of shortening the decoction time an

d saving traditional Chinese medicine resources. The results of this study lay a research foundation for “Zungu” to develop its preparation.

关键词

Keywords

references

国家中医药管理局 . 关于发布《古代经典名方目录（第一批）》的通知 [EB/OL ] .（ 2018-04-13 ）[ 2022-12-19 ] . http：//kjs.satcm.gov.cn/zhengcewenjian/2018-04-16/7107.html http://kjs.satcm.gov.cn/zhengcewenjian/2018-04-16/7107.html .

郑文江，彭紫荆，严倩，等 . 桑白皮汤加减治疗慢性阻塞性肺疾病急性加重期的Meta分析和试验序贯分析 [J ] . 中国中药杂志， 2019 ， 44 （ 17 ）： 3806 - 3815 .

刘默 . 桑白皮汤文献研究与干预LPS诱导小鼠ALI模型和斑马鱼氧化应激模型的效应及机制研究 [D ] . 南京：南京中医药大学， 2021 .

国家药品监督管理局 . 国家药品监督管理局关于发布古代经典名方中药复方制剂简化注册审批管理规定的公告：2018年第27号 [EB/OL ] .（ 2018-06-01 ）[ 2022-12-19 ] . https：//www.nmpa.gov.cn/xxgk/ggtg/qtggtg/2018060116- 3901361.html https://www.nmpa.gov.cn/xxgk/ggtg/qtggtg/2018060116-3901361.html .

陈浩，宋菊，杨平，等 . 《按古代经典名方目录管理的中药复方制剂药学研究技术指导原则（试行）》简介 [J ] . 中国食品药品监管， 2021 （ 9 ）： 78 - 87 .

卫生部，国家中医药管理局 . 关于印发《医疗机构中药煎药室管理规范》的通知 [EB/OL ] . （ 2009-03-16 ）[ 2022-12-19 ] . http：//www.satcm.gov.cn/yizhengsi/gongzuodongtai/2018-03-25/6577.html http://www.satcm.gov.cn/yizhengsi/gongzuodongtai/2018-03-25/6577.html .

文谨，刘起华，彭智平，等 . 黄芩煮散与传统饮片有效成分的煎出效果比较 [J ] . 中国实验方剂学杂志， 2014 ， 20 （ 11 ）： 34 - 36 .

刘月，罗娟，顾永哲，等 . 基于多主要成分含量测定的银翘散煮散与饮片煎煮过程比较研究 [J ] . 中国中药杂志， 2022 ， 47 （ 14 ）： 3788 - 3797 .

丘光明，邱隆，杨平 . 中国科学技术史：度量衡卷 [M ] . 北京：科学出版社， 2001 ： 217 ， 447 .

刘默，张一鸣，范欣生 . 桑白皮汤成方考证 [J ] . 中华中医药杂志， 2022 ， 37 （ 2 ）： 1065 - 1068 .

仝小林，张家成，穆兰澄，等 . 恢复煮散节省药材 [J ] . 中国新药杂志， 2012 ， 21 （ 5 ）： 470 - 474 .

鲍慧玮，王月洁，周灿灿，等 . HPLC测定桑白皮汤中桑皮苷A的含量 [J ] . 长春师范大学学报， 2021 ， 40 （ 6 ）： 42 - 45 .

国家药典委员会 . 中华人民共和国药典：一部 [S ] .2020年版. 北京：中国医药科技出版社， 2020 ： 124 - 353 .

胡钟姣，彭皓，郑露露，等 . 基于生物分子网络探讨桑白皮汤治疗慢性阻塞性肺疾病的作用机制 [J ] . 现代药物与临床， 2021 ， 36 （ 8 ）： 1556 - 1566 .

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⁰

Comparison of characteristic chromatogram and the contents of multiple indicator components of Morus alba decoction powder and decoction at different decoction time

DOI：10.6039/j.issn.1001-0408.2023.05.10

摘要

Abstract

关键词

Keywords

references