Quality evaluation of total flavonoids of <italic style="font-style: italic">Lamiophlomis rotata</italic> from different producing areas

CHEN Ruixin; JIANG Yunbin; CHEN Wenli; KANG Diandian; LI Rui; JIANG Guihua

doi:10.6039/j.issn.1001-0408.2023.04.07

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Quality evaluation of total flavonoids of Lamiophlomis rotata from different producing areas

更新时间：2025-09-27

- Quality evaluation of total flavonoids of Lamiophlomis rotata from different producing areas
- ZHONGGUO YAOFANG Vol. 34, Issue 4, Pages: 419-422(2023)
- 作者机构：
  
  1.成都中医药大学药学院/西南特色中药资源国家重点实验室，成都　611137
  2.西南大学药学院中医药学院，重庆　400715
  3.成都中医药大学国学院，成都　611137
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2023.04.07
  CLC： R917
- Received：14 July 2022，
  
  Revised：2022-12-23，
  
  Published：28 February 2023
- 稿件说明：
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陈瑞鑫,蒋运斌,陈文莉,等.不同产地独一味总黄酮的质量评价[J].中国药房,2023,34(04):419-422.

CHEN Ruixin,JIANG Yunbin,CHEN Wenli,et al.Quality evaluation of total flavonoids of Lamiophlomis rotata from different producing areas[J].ZHONGGUO YAOFANG,2023,34(04):419-422.
陈瑞鑫,蒋运斌,陈文莉,等.不同产地独一味总黄酮的质量评价[J].中国药房,2023,34(04):419-422. DOI： 10.6039/j.issn.1001-0408.2023.04.07.

CHEN Ruixin,JIANG Yunbin,CHEN Wenli,et al.Quality evaluation of total flavonoids of Lamiophlomis rotata from different producing areas[J].ZHONGGUO YAOFANG,2023,34(04):419-422. DOI： 10.6039/j.issn.1001-0408.2023.04.07.

摘要

目的

评价不同产地独一味总黄酮的质量。

方法

采用渗漉法提取、聚酰胺柱纯化S1～S15批次不同产地的独一味总黄酮。采用紫外分光光度法测定独一味总黄酮含量，并计算纯度。通过《中药色谱指纹图谱相似度评价系统（2012版）》软件建立15批独一味总黄酮的高效液相色谱指纹图谱和对照指纹图谱，并分析相似度。采用聚类分析、主成分分析、正交偏最小二乘法-判别分析评价15批独一味总黄酮的质量，并分析影响质量的主要成分。

结果

15批独一味总黄酮纯度为55.82%～94.12%，平均值为77.72%；其指纹图谱中共确定了5个共有峰，3号峰为木犀草苷，与对照指纹图谱的相似度在0.925～1.000之间；聚类分析将S1、S3～S9聚为第1类，为青海省和西藏自治区样品；S14、S15聚为第2类，均为云南省样品；S10～S13聚为第3类，均为四川省样品；S2聚为第4类；主成分分析得到第1类和第4类质量较优；正交偏最小二乘法-判别分析筛选出2、3、5号峰为引起质量差异的主要成分。

结论

青海省和西藏自治区的独一味总黄酮质量较好。

Abstract

OBJECTIVE

To evaluate the quality of total flavonoids of

Lamiophlomis rotata

from different producing areas.

METHODS

Total flavonoids of S1-S15 batches of

L. rotata

from different producing areas were extracted by percolation and purified by polyamide column. The content of total flavonoids was determined by UV spectrophotometry， and its purity was calculated. HPLC fingerprint chromatograms and control fingerprint of total flavonoids from 15 batches of

L. rotata

were established with

Similarity Evaluation System of Chromatographic Fingerprint of TCM

（2012 version）. Their similarities were analyzed. Cluster analysis， principal component analysis and orthogonal partial least squares-discriminant analysis were used to evaluate the quality of total flavonoids from 15 batches of

L. rotata，

and the main components which affected the quality of total flavonoids were analyzed.

RESULTS

The purities of total flavonoids from 15 batches of

L. rotata

were 55.82%-94.12%， with an average value of 77.72%； a total of five common peaks were identified in the fingerprint， and No. 3 peak was luteolin； the simil

arities between the fingerprint of each batch of samples and the control fingerprint were 0.925-1.000. By cluster analysis， S1 and S3-S9 were clustered into the first class， which were samples from Qinghai Province and Tibet Autonomous Region. S14 and S15 were clustered into the second class， which were samples from Yunnan Province. S10-S13 were clustered into the third class， which were all samples from Sichuan Province. S2 was clustered into the fourth class. The principal component analysis showed that the qualities of samples from the first and fourth classes were better； peaks 2， 3 and 5 were identified as the main components that caused the differences among different batches of samples by orthogonal partial least squares-discriminant analysis.

CONCLUSIONS

The qualities of total flavonoids of

L. rotata

from Qinghai Province and Tibet Autonomous Region are better.

关键词

Keywords

references

CUI Z H ， QIN S S ， ZANG E H ， et al . Traditional uses，phytochemistry，pharmacology and toxicology of Lamiophlomis rotata （Benth.）kudo：a review [J ] . RSC Adv ， 2020 ， 10 （ 19 ）： 11463 - 11474 .

刘星雨，周敏，孙体健 . 天然黄酮类化合物的药理活性及分离提取 [J ] . 中国药物与临床， 2014 ， 14 （ 5 ）： 621 - 624 .

潘正，向浏欣，刘圣蓉，等 . 独一味化学成分的研究 [J ] . 中成药， 2018 ， 40 （ 3 ）： 629 - 632 .

郑亚男，杜文杰，尹雪霏，等 . 独一味不同有效部位抗炎镇痛作用比较 [J ] . 时珍国医国药， 2015 ， 26 （ 2 ）： 282 - 284 .

JIANG Y B ， ZHONG M ， LONG F ， et al . Network pharmacology-based prediction of active ingredients and mechanisms of Lamiophlomis rotata （Benth.）kudo against rheumatoid arthritis [J ] . Front Pharmacol ， 2019 ， 10 ： 1435 .

HOU Y N ， WU J C ， HUANG Q ， et al . Luteolin inhibits proliferation and affects the function of stimulated rat synovial fibroblasts [J ] . Cell Biol Int ， 2009 ， 33 （ 2 ）： 135 - 147 .

张燕飞，陈瑞鑫，袁茂华，等 . 藏药独一味抗类风湿关节炎的作用及其药效物质基础研究 [J ] . 中国药房， 2021 ， 32 （ 5 ）： 578 - 583 .

张泉龙，邱建国，李茂星，等 . 独一味环烯醚萜苷外用止血作用研究 [J ] . 医药导报， 2011 ， 30 （ 7 ）： 877 - 879 .

桑育黎，郝延军，陈沉，等 . 独一味镇痛止血有效部位的研究 [J ] . 时珍国医国药， 2011 ， 22 （ 5 ）： 1126 - 1127 .

邱建国，张泉龙，李茂星，等 . 独一味不同提取物中总环烯醚萜苷、总黄酮和总苯乙醇苷的测定 [J ] . 中国实验方剂学杂志， 2013 ， 19 （ 23 ）： 119 - 124 .

匡海学 . 中药化学 [M ] . 3版 . 北京：中国中医药出版社， 2017 ： 135 .

李军茂，何明珍，冯育林，等 . 木犀草素及木犀草苷在大鼠体内的代谢研究 [J ] . 中药新药与临床药理， 2017 ， 28 （ 1 ）： 61 - 68 .

龚伟伟，罗光明，秦倩，等 . 茶芎HPLC指纹图谱的建立及差异性成分的含量测定 [J ] . 中国药房， 2022 ， 33 （ 16 ）： 1968 - 1973 .

王永，王世华，安军永，等 . 大孔树脂分离纯化独一味总黄酮的工艺研究 [J ] . 中国药业， 2012 ， 21 （ 5 ）： 25 - 27 .

冯爱博，杨光，贺亮，等 . 聚酰胺树脂纯化竹壳类黄酮工艺的研究 [J ] . 工业微生物， 2019 ， 49 （ 3 ）： 27 - 32 .

中国科学院中国植物志编辑委员会 . 中国植物志：第65（2）卷 [M ] . 北京：科学出版社， 1997 ： 480 .

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⁰

Quality evaluation of total flavonoids of Lamiophlomis rotata from different producing areas

DOI：10.6039/j.issn.1001-0408.2023.04.07

摘要

Abstract

关键词

Keywords

references