Study on protective effect and mechanism of Arisaema Cum Bile on acute liver injury induced by CCl<sub>4</sub> in mice

SHI Congjing; DENG Yafang; ZHANG Zhihong; LI Biao; SU Huilin; PENG Donghui; ZENG Yuanning; WANG Qiuhong

doi:10.6039/j.issn.1001-0408.2022.23.04

您当前的位置：

首页 >

文章列表页 >

Study on protective effect and mechanism of Arisaema Cum Bile on acute liver injury induced by CCl₄ in mice

更新时间：2025-09-26

- Study on protective effect and mechanism of Arisaema Cum Bile on acute liver injury induced by CCl₄ in mice
- ZHONGGUO YAOFANG Vol. 33, Issue 23, Pages: 2835-2839(2022)
- 作者机构：
  
  1.广东药科大学中药学院/广东省中药饮片规范化炮制工程技术研究中心，广州　510006
  2.黑龙江中医药大学药学院/教育部北药基础与应用研究重点实验室/黑龙江中药及天然药物药效物质基础研究重点实验室，哈尔滨　150040
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2022.23.04
  CLC： R965
- Received：29 August 2022，
  
  Revised：2022-10-31，
  
  Published：15 December 2022
- 稿件说明：
移动端阅览
史丛晶,邓雅方,张志宏,等.胆南星对四氯化碳致小鼠急性肝损伤的保护作用及机制研究[J].中国药房,2022,33(23):2835-2839.

SHI Congjing,DENG Yafang,ZHANG Zhihong,et al.Study on protective effect and mechanism of Arisaema Cum Bile on acute liver injury induced by CCl₄ in mice[J].ZHONGGUO YAOFANG,2022,33(23):2835-2839.
史丛晶,邓雅方,张志宏,等.胆南星对四氯化碳致小鼠急性肝损伤的保护作用及机制研究[J].中国药房,2022,33(23):2835-2839. DOI： 10.6039/j.issn.1001-0408.2022.23.04.

SHI Congjing,DENG Yafang,ZHANG Zhihong,et al.Study on protective effect and mechanism of Arisaema Cum Bile on acute liver injury induced by CCl₄ in mice[J].ZHONGGUO YAOFANG,2022,33(23):2835-2839. DOI： 10.6039/j.issn.1001-0408.2022.23.04.

摘要

目的

研究胆南星对四氯化碳（CCl

）致小鼠急性肝损伤的保护作用及可能的作用机制。

方法

将50只小鼠按体质量随机分为正常组、模型组、阳性对照组（联苯双酯滴丸，150 mg/kg）和胆南星低、高剂量组（0.78、2.34 g/kg），每组10只。每天灌胃给药1次，连续7 d。末次灌胃2 h后，除正常组外的其余各组小鼠均腹腔注射0.2% CCl

-橄榄油溶液复制急性肝损伤模型。腹腔注射17 h后，按照试剂盒方法检测小鼠血清中丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、白细胞介素6（IL-6）、肿瘤坏死因子α（TNF-α）和肝组织中丙二醛（MDA）、超氧化物歧化酶（SOD）水平，测定小鼠的肝脏指数，采用苏木精-伊红染色法观察小鼠肝组织的病理形态学变化并对肝损伤程度进行量化评分，采用实时荧光定量PCR法检测小鼠肝组织中IL-6、TNF-α mRNA表达水平，采用Western blot法检测小鼠肝组织中酪氨酸激酶2（JAK2）、信号传导及转录激活因子3（STAT3）、核因子κB p65（NF-κB p65）蛋白表达水平。

结果

与正常组比较，模型组小鼠ALT、AST、IL-6、TNF-α、MDA水平和肝脏指数显著升高（

＜0.05），SOD水平显著降低（

＜0.05）；IL-6、TNF-α mRNA及JAK2、STAT3、NF-κB p65蛋白表达显著上调（

＜0.05）；肝索结构严重紊乱，肝细胞有较多炎症细胞浸润，肝损伤程度评分显著升高（

＜0.05）。与模型组比较，胆南星低、高剂量组小鼠肝组织病理变化及上述指标水平均显著改善（

＜0.05）。

结论

胆南星对CCl

致小鼠急性肝损伤具有保护作用，其机制可能与抑制JAK2/STAT3/NF-κB信号通路介导的炎症反应及抗氧化应激作用有关。

Abstract

OBJECTIVE

To investigate the protective effect and potential mechanism of Arisaema Cum Bile on acute liver injury induced by carbon tetrachloride （CCl

） in mice.

METHODS

Fifty mice were randomly divided into normal group， model group， positive control group （Biphenyl diester dropping pills， 150 mg/kg）， Arisaema

Cum Bile low-dose and high-dose groups （0.78， 2.34 g/kg）， with 10 mice in each group. The mice in each group were given relevant medicine intragastrically， once a day， for 7 consecutive days. Two hours after the last administration， those groups were given intraperitoneal injection of 0.2% CCl

-olive oil solution to induce acute liver injury model except for normal group. Seventeen hours after intraperitoneal injection， the serum levels of alanine aminotransferase （ALT）， aspartate aminotransferase （AST）， interleukin-6 （IL-6）， tumor necrosis factor-α（TNF-α）， and malondialdehyde （MDA）， superoxide dismutase （SOD） in liver tissue were measured with kit method. The hepatic index was detected. The pathological changes of liver tissue were observed by HE staining， and the degree of liver injury was scored quantitatively. The mRNA expressions of TNF-α and IL-6 in liver tissue were detected by real-time fluorescence quantitative PCR； the protein expressions of Janus kinase 2 （JAK2）， signal transducer and activator of transcription protein 3 （STAT3） and nuclear factor-κB p65 （NF-κB p65） in liver tissue were detected by Western blot assay.

RESULTS

Compared with normal group， the levels of ALT， AST， IL-6， TNF-α and MDA， the hepatic index were increased significantly （

＜0.05）， while the level of SOD was decreased significantly （

＜0.05）； the mRNA expressions of IL-6 and TNF-α， and the protein expressions of JAK2， STAT3 and NF-κB p65 were up-regulated significantly （

＜0.05）； the pathological observation of liver tissue showed that the structure of hepatic cord was seriously disordered， there were many inflammatory cells infiltration of liver cells， and the liver injury score was significantly increased （

＜0.05）. Compared with model group， pathological changes and above indexes in mice were improved significantly in Arisaema Cum Bile low-dose and high-dose groups （

＜0.05）.

CONCLUSIONS

Arisaema Cum Bile has a protective effect on CCl

-induced acute liver injury in mice， which may be related to the inhibition of inflammatory response mediated by JAK2/STAT3/NF-κB signal pathway and antioxidant stress.

关键词

Keywords

references

张红阳，李雪溦，姚雪莲，等 . 肝损伤的分子机制及其中药药理研究进展 [J ] . 中药新药与临床药理， 2016 ， 27 （ 3 ）： 448 - 455 .

刘文静，高进贤，钱倩，等 . 白皮杉醇抑制内质网应激保护四氯化碳诱导的小鼠急性肝损伤 [J ] . 中成药， 2020 ， 42 （ 12 ）： 3158 - 3165 .

李晓亮，匡海学，孟永海，等 . 商陆总皂苷对四氯化碳诱导的小鼠急性肝损伤的作用 [J ] . 中华中医药杂志， 2018 ， 33 （ 2 ）： 649 - 652 .

赵芳 . TNF-α介导的NF-κB通路在肝纤维化中的作用及中药对其影响 [D ] . 大连：大连医科大学， 2012 .

国家药典委员会 . 中华人民共和国药典：一部 [S ] . 2020年版. 北京：中国医药科技出版社， 2020 ： 222 .

唐照琦，李彪，王秋红，等 . 胆南星的化学成分、药理作用及相关复方临床应用的研究进展 [J ] . 中国药房， 2020 ， 31 （ 12 ）： 1523 - 1527 .

刘晓月，陶鑫，潘多，等 . 胆南星化学成分的研究 [J ] . 中成药， 2018 ， 40 （ 9 ）： 1991 - 1995 .

胡凤娇，宋文杰，王张，等 . 四氯化碳致小鼠急性肝损伤模型造模要素及中医药防治的数据挖掘研究 [J ] . 中药与临床， 2018 ， 9 （ 5 ）： 34 - 37，44 .

WANG F ， XUE Y ， YANG J Y ， et al . Hepatoprotective effect of apple polyphenols against concanavalin A induced immunological liver injury in mice [J ] . Chem Biol Interact ， 2016 ， 258 ： 159 - 165 .

AJUEBOR M N ， CAREY J A ， SWAIN M G . CCR5 in T cell-mediated liver diseases：what’s going on? [J ] . J Immunol ， 2006 ， 177 （ 4 ）： 2039 - 2045 .

LIU L J ， XU M ， ZHU J ， et al . Adiponectin alleviates liver injury in sepsis rats through AMPK/mTOR pathway [J ] . Eur Rev Med Pharmacol Sci ， 2020 ， 24 （ 20 ）： 10745 - 10752 .

王诗洋，王枭，徐祖清，等 . 苦杏仁苷通过抑制氧化应激及炎症反应减轻四氯化碳诱导的大鼠肝纤维化作用 [J ] . 现代免疫学， 2020 ， 40 （ 6 ）： 471 - 475，481 .

隋菱，郑静彬，蔡国弟，等 . 姜黄素对四氯化碳诱导大鼠急性肝损害的保护作用 [J ] . 中国现代应用药学， 2017 ， 34 （ 11 ）： 1517 - 1521 .

冯锦，吴建红，熊响莲，等 . 紫杉醇通过调控JAK2/STAT3通路对肝纤维化模型大鼠Th17/Treg影响的研究 [J ] . 中国免疫学杂志， 2022 ， 38 （ 13 ）： 1564 - 1569 .

HAO J H ， SUN W L ， XU H C . Pathogenesis of concanavalin A induced autoimmune hepatitis in mice [J ] . Int Immunopharmacol ， 2022 ， 102 ： 108411 .

Views

143

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effects and mechanism of plumbagin on the inflammatory response and oxidative stress in rats with AECOPD

Research progress on mechanism of antidepressant action of curcumin

Effect and mechanism of Sanqi danshen tablets in the treatment of non-alcoholic fatty liver disease

Effects and mechanism of Hugan buzure formula on intrahepatic cholestatic liver injury in rats

Related Author

LI Shirong

XU Peipei

WANG Yaru

ZHOU Ce

FU Hao

XI Yuting

ZHOU Jianping

LEI Yutian

Related Institution

Dept. of Respiratory and Critical Care Medicine， Jiangbei Campus， Zhongda Hospital Southeast University

Dept. of Hematology， Nanjing Drum Tower Hospital

Dept. of Anorectology， Hospital of Chengdu University of Traditional Chinese Medicine

Dept. of Anorectology， Zigong Hospital of Traditional Chinese Medicine

Dept. of Clinical Pharmacy Center， the First Affiliated Hospital of Kunming Medical University

⁰

Study on protective effect and mechanism of Arisaema Cum Bile on acute liver injury induced by CCl4 in mice

DOI：10.6039/j.issn.1001-0408.2022.23.04

摘要

Abstract

关键词

Keywords

references

Study on protective effect and mechanism of Arisaema Cum Bile on acute liver injury induced by CCl₄ in mice