Improvement effects and mechanism of gentian iridoids on lipid accumulation and inflammation in NAFLD cell model

WANG Lijuan; WENG Lili; XIAO Chunping; YU Qian; KOU Baixin

doi:10.6039/j.issn.1001-0408.2022.19.10

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Improvement effects and mechanism of gentian iridoids on lipid accumulation and inflammation in NAFLD cell model

更新时间：2025-12-03

- Improvement effects and mechanism of gentian iridoids on lipid accumulation and inflammation in NAFLD cell model
- ZHONGGUO YAOFANG Vol. 33, Issue 19, Pages: 2354-2358(2022)
- 作者机构：
  
  长春中医药大学药学院，长春　130117
- 作者简介：
- 基金信息：
- DOI：10.6039/j.issn.1001-0408.2022.19.10
  CLC： R965
- Received：21 March 2022，
  
  Revised：2022-08-24，
  
  Published：15 October 2022
- 稿件说明：
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王丽娟,翁丽丽,肖春萍等.龙胆环烯醚萜类成分对NAFLD细胞模型脂质积累及炎症的改善作用及机制 ^Δ[J].中国药房,2022,33(19):2354-2358.

WANG Lijuan,WENG Lili,XIAO Chunping,et al.Improvement effects and mechanism of gentian iridoids on lipid accumulation and inflammation in NAFLD cell model[J].ZHONGGUO YAOFANG,2022,33(19):2354-2358.
王丽娟,翁丽丽,肖春萍等.龙胆环烯醚萜类成分对NAFLD细胞模型脂质积累及炎症的改善作用及机制 ^Δ[J].中国药房,2022,33(19):2354-2358. DOI： 10.6039/j.issn.1001-0408.2022.19.10.

WANG Lijuan,WENG Lili,XIAO Chunping,et al.Improvement effects and mechanism of gentian iridoids on lipid accumulation and inflammation in NAFLD cell model[J].ZHONGGUO YAOFANG,2022,33(19):2354-2358. DOI： 10.6039/j.issn.1001-0408.2022.19.10.

摘要

目的

研究龙胆环烯醚萜类成分（gentian iridoids，GI）对非酒精性脂肪性肝病（NAFLD）细胞模型脂质积累及炎症的改善作用及机制。

方法

以游离脂肪酸（0.5 mmol/L）诱导的人肝癌HepG2细胞为NAFLD细胞模型。考察不同浓度（0.125、0.25、0.5、1、2、4 mg/mL）GI对HepG2细胞活力的影响。将HepG2细胞分为对照组、模型组和GI 低、中、高浓度组（0.25、0.5、1 mg/mL），除对照组外，其余组均加入0.5 mmol/L游离脂肪酸进行造模。培养24 h后，考察HepG2细胞内脂滴形成的情况，检测细胞内三酰甘油（TG）含量，检测细胞上清液中天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）和炎症指标[白细胞介素1β（IL-1β）、IL-6、肿瘤坏死因子α（TNF-α）]的水平；检测细胞中固醇调节元件结合蛋白1c（SREBP-1c）、脂肪酸合成酶（FAS）mRNA和核因子κB（NF-κB）信号通路相关蛋白[ NF-κB抑制蛋白α（IκBα）、NF-κB、磷酸化NF-κB（p-NF-κB）]的表达。

结果

HepG2细胞经不同浓度GI作用后，细胞活力均显著升高（

＜0.01）。与模型组比较，GI各浓度组细胞内红色脂滴明显减少，细胞核萎缩不明显、体积大小正常；细胞内TG含量（低浓度组除外），细胞上清液中AST、ALT、IL-1β、IL-6（低剂量组除外）、TNF-α水平，细胞中SREBP-1c、FAS mRNA表达水平以及NF-κB蛋白磷酸化水平均显著降低（

＜0.05或

＜0.01），IκBα蛋白表达水平均显著升高（

＜0.01）。

结论

GI可减少NAFLD细胞模型的脂质积累，减轻炎症反应，其作用机制可能与抑制SREBP-1c/FAS及NF-κB信号通路有关。

Abstract

OBJECTIVE

To study the improvement effects and mechanism of gentian iridoids （GI） on lipid accumulation and inflammation in non-alcoholic fatty liver disease （NAFLD） model.

METHODS

Human hepatoma HepG2 cells induced by free fatty acids （0.5 mmol/L） were selected as NAFLD cell models. The effects of GI at different concentrations （0.125，0.25，0.5，1，2，4 mg/mL） on HepG2 cell viability were investigated. HepG2 cells were divided into control group， model group and low， medium and high concentration groups of GI （0.25， 0.5 and 1 mg/mL）. Except for control group， 0.5 mmol/L free fatty acid was added in other groups for modeling.After 24 h of culture， the formation of lipid droplets was observed in HepG2 cells；the content of triglyceride （TG） was detected. The levels of aspartate aminotransferase （AST）， alanine aminotransferase （ALT） and inflammation indexes [interleukin-1 β （IL-1 β）， IL-6， tumor necrosis factor α （TNF-α）] in cell supernatant were determined. The mRNA expressions of sterol-regulatory element binding protein （SREBP-1c） and fatty acid synthase （FAS） as well as the expression of nuclear factor-κB（NF-κB） related proteins [NF-κB inhibitor protein α （IκBα）， NF-κB，phosphorylated NF-κB （p-NF-κB）] were also detected.

RESULTS

After treated with different concentrations of GI， the cell viability of HepG2 cells was improved significantly （

＜0.01）. Compared with model group， the number of red lipid droplets in the cells of each concentration group of GI was significantly reduced， the nuclear atrophy was not obvious， and the size was normal；the content of TG （except for low concentration group）， and the levels of ALT， AST， IL-1β， IL-6 （except for low concentration group） and TNF-α in cell supernatant were all decreased significantly； mRNA expression of SREBP-1c and FAS and the phosphorylation level of NF-κB were also decreased significantly （

＜0.05 or

＜0.01）； the protein expression of IκBα was increased significantly （

＜0.01）.

CONCLUSIONS

GI can reduce the lipid accumulation and inflammatory response of NAFLD cell model induced by free fatty acids， and its mechanism may be related to the inhibition of SREBP-1c/FAS and NF-κB signaling pathway.

关键词

Keywords

references

LEM H ， YEOY H ， LI X H ， et al . 2019 global NAFLD prevalence： asystematic review and meta-analysis [J/OL ] . Clin Gastroenterol Hepatol ， 2021 [ 2022-07-23 ] . https：//pubmed.ncbi.nlm.nih.gov/34890795/.DOI：10.1016/j.cgh.2021.12.002. Online ahead of print https://pubmed.ncbi.nlm.nih.gov/34890795/.DOI:10.1016/j.cgh.2021.12.002.Onlineaheadofprint .

IPSEND H ， LYKKESFELDT J ， TVEDEN-NYBORG P . Molecular mechanisms of hepatic lipid accumulation in non-alcoholic fatty liver disease [J ] . Cell Mol Life Sci ， 2018 ， 75 （ 18 ）： 3313 - 3327 .

POWELLE E ， WONGV W S ， RINELLA M . Non-alcoholic fatty liver disease [J ] . Lancet ， 2021 ， 397 （ 10290 ）： 2212 - 2224 .

HEIDA A ， GRUBEN N ， CATRYSSE L ， et al . The hepatocyte IKK：NF-κB axis promotes liver steatosis by stimula- ting de novo lipogenesis and cholesterol synthesis [J ] . Mol Metab ， 2021 ， 54 ： 101349 .

ZHANG S M ， MENG G ， ZHANG Q ， et al . Inflammatory potential of diet and risk of nonalcoholic fatty liver disease：a prospective cohort study [J ] . Eur J Clin Nutr ， 2022 ， 76 （ 8 ）： 1125 - 1132 .

SINGH S ， OSNAN A ， KHARBANDAK K . Treatment options for alcoholic and non-alcoholic fatty liver disease：a review [J ] . World J Gastroenterol ， 2017 ， 23 （ 36 ）： 6549 - 6570 .

潘旭，朱鹤云，张昌浩，等 . 龙胆化学成分和药理作用研究进展 [J ] . 吉林医药学院学报， 2020 ， 41 （ 2 ）： 150 - 151 .

赵兴华 . 滋肾益肝方及龙胆泻肝汤联合多烯磷脂酰胆碱治疗急性药物性肝损伤临床研究 [J ] . 实用中医药杂志， 2021 ， 37 （ 10 ）： 1699 - 1701 .

WANG C C ， GONG X ， BO A ， et al . Iridoids：research advances in their phytochemistry，biological activities，and pharmacokinetics [J ] . Molecules ， 2020 ， 25 （ 2 ）： E287 .

唐利东，王健英，谭丹，等 . 杜仲三萜、环烯醚萜类成分对胶原诱导性关节炎大鼠的影响 [J/OL ] . 中国中药杂志， 2022 [ 2022-08-22 ] . https：//kns.cnki.net/kcms/detail/detail.aspx?dbcode=CAPJ&dbname=CAPJLAST&filename=ZGZY20220601003&uniplatform=NZKPT&v=zoPwKB8LthDYcKOlz_Fknmx0Qdw0R9Ii21no-cGC4BaKr- brobpz3Me5_6BodMtcV. DOI：10.19540/j.cnki.cjcmm. 20220401.703 https://kns.cnki.net/kcms/detail/detail.aspx?dbcode=CAPJ&dbname=CAPJLAST&filename=ZGZY20220601003&uniplatform=NZKPT&v=zoPwKB8LthDYcKOlz_Fknmx0Qdw0R9Ii21no-cGC4BaKr-brobpz3Me5_6BodMtcV.DOI:10.19540/j.cnki.cjcmm.20220401.703 .

KANURI G ， BERGHEIM I . In vitro and in vivo models of non-alcoholic fatty liver disease （NAFLD） [J ] . Int J Mol Sci ， 2013 ， 14 （ 6 ）： 11963 - 11980 .

邢菊玲，刘芬，冯萌，等 . 桑根酮C对游离脂肪酸诱导人肝癌HepG2细胞脂质蓄积的改善作用 [J ] . 中国药房， 2021 ， 32 （ 15 ）： 1868 - 1873 .

ZHANG W J ， LIN H ， CHENG W L ， et al . Protective effect and mechanism of plant-based monoterpenoids in non-alcoholic fatty liver diseases [J ] . J Agric Food Chem ， 2022 ， 70 （ 16 ）： 4839 - 4859 .

ZHENG Y Y ， FANG D ， HUANG C Y ， et al . Gentiana scabra restrains hepatic pro-inflammatory macrophages to ameliorate non-alcoholic fatty liver disease [J ] . Front Pharmacol ， 2021 ， 12 ： 816032 .

MIREAA M ， TACKC J ， CHAVAKIS T ， et al . IL-1 family cytokine pathways underlying NAFLD：towards new treatment strategies [J ] . Trends Mol Med ， 2018 ， 24 （ 5 ）： 458 - 471 .

BAGHERIEH M ， KHEIROLLAHI A ， ZAMANI-GARMSIRI F ， et al . Folic acid ameliorates palmitate-induced inflammation through decreasing homocysteine and inhibiting NF-κB pathway in HepG2 cells [J ] . Arch Physiol Biochem ， 2021 ：2021Feb 17 ； 1 - 2021 Feb17；8.

GEETHANGILI M ， LINC W ， MERSMANNH J ， et al . Methyl brevifolincarboxylate attenuates free fatty acid-induced lipid metabolism and inflammation in hepatocytes through AMPK/NF-κB signaling pathway [J ] . Int J Mol Sci ， 2021 ， 22 （ 18 ）： 10062 .

LI L F ， ZHANG X Y ， REN H J ， et al . miR-23a/b-3p promotes hepatic lipid accumulation by regulating Srebp-1c and Fas [J ] . J Mol Endocrinol ， 2021 ， 68 （ 1 ）： 35 - 49 .

WANG Y H ， VISCARRA J ， KIMS J ， et al . Transcriptional regulation of hepatic lipogenesis [J ] . Nat Rev Mol Cell Biol ， 2015 ， 16 （ 11 ）： 678 - 689 .

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