Transplantation of human umbilical cord mesenchymal stem cells repairs radiation-induced reproductive injury in male mice <italic style="font-style: italic">via</italic> improving mitochondrial function and attenuating oxidative stress

LI Jiangyi; ZHENG Bo; WANG Yuxin; GUO Ling; DING Guirong

doi:10.16016/j.2097-0927.202510053

您当前的位置：

首页 >

文章列表页 >

Transplantation of human umbilical cord mesenchymal stem cells repairs radiation-induced reproductive injury in male mice via improving mitochondrial function and attenuating oxidative stress

Basic Medicine | 更新时间：2026-03-02

- Transplantation of human umbilical cord mesenchymal stem cells repairs radiation-induced reproductive injury in male mice via improving mitochondrial function and attenuating oxidative stress
- Journal of Army Medical University Vol. 48, Issue 4, Pages: 433-444(2026)
- 作者机构：
  
  1.山东第二医科大学公共卫生学院，山东潍坊
  2.空军军医大学预防医学系辐射防护医学教研室，特殊作业环境危害评估与防治教育部重点实验室，陕西西安
- 作者简介：
- 基金信息：
  
  the National Natural Science Foundation for Young Scholars of China(82304068)
- DOI：10.16016/j.2097-0927.202510053
  CLC： R329.21;R456;R818.87
- Received：19 October 2025，
  
  Revised：2026-01-08，
  
  Accepted：16 January 2026，
  
  Published：28 February 2026
- 稿件说明：
移动端阅览
李姜怡，郑博，王宇欣，等.人脐带间充质干细胞移植通过改善线粒体功能与减轻氧化应激修复雄性小鼠放射性生殖损伤［J］.陆军军医大学学报，2026，48（04）：433-444.DOI：10.16016/j.2097-0927.202510053.

LI J G，ZHENG B，WANG Y X，et al.Transplantation of human umbilical cord mesenchymal stem cells repairs radiation-induced reproductive injury in male mice via improving mitochondrial function and attenuating oxidative stress［J］.J Army Med Univ，2026，48（04）：433-444.DOI：10.16016/j.2097-0927.202510053.
李姜怡，郑博，王宇欣，等.人脐带间充质干细胞移植通过改善线粒体功能与减轻氧化应激修复雄性小鼠放射性生殖损伤［J］.陆军军医大学学报，2026，48（04）：433-444.DOI：10.16016/j.2097-0927.202510053. DOI：

LI J G，ZHENG B，WANG Y X，et al.Transplantation of human umbilical cord mesenchymal stem cells repairs radiation-induced reproductive injury in male mice via improving mitochondrial function and attenuating oxidative stress［J］.J Army Med Univ，2026，48（04）：433-444.DOI：10.16016/j.2097-0927.202510053. DOI：

摘要

目的

探究人脐带间充质干细胞（human umbilical cord mesenchymal stem cells，hUC-MSCs）对雄性小鼠放射性生殖损伤的改善作用及其潜在机制。

方法

在获得空军军医大学西京医院妇产科健康足月妊娠孕妇知情同意并签署知情同意书后，于2024年12月在手术室无菌条件下采集1段6~8 cm长的健康足月新生儿的脐带组织，用于分离培养hUC-MSCs。将45只6~8周龄健康雄性C57BL/6小鼠［体质量（22±2）g］通过随机数字表法分为对照+尾静脉注射PBS组（Control+PBS，

=3）、对照+尾静脉注射hUC-MSCs组（Control+MSCs，

=6）、照射+尾静脉注射hUC-MSCs组（Rad+MSCs，

=6）、对照组（Control，

=10）、照射组（Rad，

=10）及照射+干细胞干预组（Rad+hUC-MSCs，

=10）。使用

Co γ射线对Rad组、Rad+MSCs组和Rad+hUC-MSCs组小鼠进行全身单次照射，剂量率1.28 Gy/min，总剂量6 Gy。照射结束后，Rad+MSCs组和Rad+hUC-MSCs组小鼠立即经尾静脉单次注射hUC-MSCs悬液（2×10

/200 μL）。采用多模式动物活体成像系统及共聚焦荧光显微镜观察小鼠睾丸组织中hUC-MSCs的分布情况。照射后12周，取材并剥离双侧睾丸组织进行称量，计算睾丸系数；显微镜下评估小鼠精子质量；采用苏木精-伊红（hematoxylin and eosin staining，HE）染色观察小鼠睾丸形态结构；通过流式细胞术评估小鼠睾丸组织活性氧（reacti

ve oxygen species，ROS）及线粒体膜电位（mitochondrial membrane potential，MMP）水平；通过比色法检测睾丸组织中丙二醛（malondialdehyde，MDA）、总超氧化物歧化酶（total super oxide dismutase，T-SOD）和过氧化氢酶（catalase，CAT）含量；应用TUNEL染色检测睾丸细胞凋亡情况；采用Western blot检测睾丸组织中E3泛素蛋白连接酶帕金蛋白（E3 ubiquitin-protein ligase parkin，Parkin）、磷酸酶和张力蛋白同源激酶1（PTEN-induced putative kinase 1，PINK1）、苄氯素1（Beclin1）、线粒体动力蛋白样GTP酶视神经萎缩蛋白1（OPA1 mitochondrial dynamin-like GTPase，OPA1）、线粒体动力相关蛋白1（dynamin-related protein 1，Drp1）、Bcl-2相关X蛋白（Bcl2-associated x protein，Bax）、B淋巴细胞瘤-2（B cell lymphoma-2，Bcl-2）的表达情况。

结果

照射后24、48 h，多模式动物活体成像系统观察发现，Control+PBS组小鼠睾丸组织未见荧光信号，Control+MSCs组与Rad+MSCs组小鼠睾丸组织中均可见荧光信号，进一步通过共聚焦荧光显微镜观察发现，在Control+MSCs组与Rad+MSCs组小鼠睾丸间质区域可见红色荧光，检测到荧光染料标记的hUC-MSCs信号。照射后12周，与Control组相比，Rad组小鼠精子数量（

0.05）和活力（

0.01）显著降低，畸形率显著升高（

0.001）；睾丸生精细胞出现空泡化且排列紊乱，生精小管直径和生精上皮厚度减小（

0.001）；睾丸组织ROS水平升高（

0.05），MMP水平显著降低（

0.001）；MDA含量明显升高（

0.001），T-SOD活力升高（

0.05），CAT活力降低（

0.05）；睾丸组织中Parkin（

0.01）、PINK1（

0.05）、Beclin1（

0.05）、OPA1（

0.01）和Drp1（

0.05）蛋白表达升高，TUNEL

细胞增多（

0.001），Bcl-2/Bax比值降低（

0.01）。与Rad组相比，Rad+hUC-MSCs组小鼠精子数量和精子活力显著升高（

0.05），精子畸形率显著降低（

0.001）；睾丸生精细胞空泡化和排列紊乱情况得到改善，生精上皮厚度增加（

0.01）；睾丸组织ROS水平降低（

0.05），MMP水平明显升高（

0.01）；MDA含量明显减少（

0.001），T-SOD活力降低（

0.05），CAT活力升高（

0.05）；睾丸组织中Parkin、PINK1、Beclin1、OPA1和Drp1蛋白表达降低（

0.05），TUNEL

细胞减少（

0.001），Bcl-2/Bax比值升高（

0.05）。

结论

hUC-MSCs可能通过改善线粒体损伤，减轻睾丸组织氧化应激损伤和细胞凋亡，从而改善放射性雄性生殖损伤。

Abstract

Objective

To investigate the ameliorative effect of human umbilical cord mesenchymal stem cells (hUC-MSCs) on radiation-induced reproductive damage in male mice and its potential mechanisms

Methods

After obtaining informed consent and signed consent forms from healthy full-term pregnant women in the Department of Obstetrics and Gynecology at Xijing Hospital of Air Force Medical University

umbilical cord tissue approximately from 6 to 8 cm in length from healthy full-term newborns was collected under sterile conditions in the operating room for isolating and culturing hUC-MSCs; 45 healthy male C57BL/6 mice aged from 6 to 8 weeks with a body weight of 22±2 g were divided into Control+PBS (

=3)

Control+MSCs (

=6)

Rad+MSCs (

=6)

Control (

=10)

Rad (

=10)

and Rad+hUC-MSCs (

=10) groups using a random number table method; the Rad

Rad+MSCs

and Rad+hUC-MSCs groups were subjected to whole-body single-dose irradiation using

Co γ-ray at a dose rate of 1.28 Gy/min and a total dose of 6 Gy; immediately after irradiation

the Rad+MSCs and Rad+hUC-MSCs groups received a single tail vein injection of hUC-MSCs suspension (2×10⁶ cells/200 μL); the distribution of hUC-MSCs in testicular tissue was observed using a multimodal

in vivo

imaging system and confocal fluorescence microscopy; at 12 weeks post-irradiation

bilateral testes were harvested and weighed to calculate the testicular coefficient; sperm quality was assessed under a microscope; testicular morphology and structure were evaluated using Hematoxylin and Eosin (HE) staining; reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) levels in testicular tissue were measured by flow cytometry; malondialdehyde (MDA)

total superoxide dismutase (T-SOD)

and catalase (CAT) contents were detected by colorimetry; testicular cell apoptosis

was assessed using TUNEL staining; and Western blot was used to detect the expression levels of Parkin RBR E3 ubiquitin-protein ligase (Parkin)

PTEN-induced putative kinase 1 (PINK1)

Beclin1

OPA1 mitochondrial dynamin-like GTPase (OPA1)

dynamin-related protein 1 (Drp1)

Bcl2-associated X protein (Bax)

and B cell lymphoma-2 (Bcl-2)

Results

At 24 h and 48 h post-irradiation

multimodal

in vivo

imaging showed no fluorescent signals in the testicular tissue of the Control+PBS group

while fluorescent signals were observed in the Control+MSCs and Rad+MSCs groups; further confocal fluorescence microscopy revealed red fluorescence in the interstitial regions of testicular tissue in the Control+MSCs and Rad+MSCs groups

detecting fluorescence dye labeled MSCs signals; at 12 weeks post-irradiation

compared to the Control group

the Rad group showed significantly decreased sperm count (

0.05) and motility (

0.01)

significantly increased malformation rate (

0.001)

vacuolization and disordered arrangement of spermatogenic cells

reduced seminiferous tubule diameter and spermatogenic epithelium thickness (

0.001)

elevated testicular tissue ROS levels (

0.05)

significantly decreased MMP levels (

0.001)

increased MDA content (

0.001)

elevated T-SOD activity (

0.05)

decreased CAT activity (

0.05)

increased protein expression of Parkin (

0.01)

PINK1 (

0.05)

Beclin1 (

0.05)

OPA1 (

0.01)

and Drp1 (

0.05)

increased TUNEL-positive cells (

0.001)

and decreased Bcl-2/Bax ratio (

0.01); compared to the Rad group

the Rad+hUC-MSCs group exhibited significantly increased sperm count and motility (

0.05)

gnificantly decreased malformation rate (

0.001)

improved vacuolization and arrangement of spermatogenic cells

increased spermatogenic epithelium thickness (

0.01)

reduced testicular tissue ROS levels (

0.05)

significantly increased MMP levels (

0.01)

decreased MDA content (

0.001)

reduced T-SOD activity (

0.05)

increased CAT activity (

0.05)

decreased protein expression of Parkin (

0.05)

PINK1 (

0.05)

Beclin1 (

0.05)

OPA1 (

0.05)

and Drp1 (

0.05)

reduced TUNEL-positive cells (

0.001)

and increased Bcl-2/Bax ratio (

0.05)

Conclusion

hUC-MSCs may alleviate radiation-induced male reproductive damage by mitigating mitochondrial damage

reducing testicular tissue oxidative stress injury and cell apoptosis.

关键词

Keywords

references

STEWART F A ， AKLEYEV A V ， HAUER-JENSEN M ， et al . ICRP publication 118： ICRP statement on tissue reactions and early and late effects of radiation in normal tissues and organs： threshold doses for tissue reactions in a radiation protection context ［J］. Ann ICRP ， 2012 ， 41 （ 1-2 ）： 1 - 322 . DOI： 10.1016/j.icrp.2012.02.001 http://dx.doi.org/10.1016/j.icrp.2012.02.001 .

BIEDKA M ， KUŹBA-KRYSZAK T ， NOWIKIEWICZ T ， et al . Fertility impairment in radiotherapy ［J］. Contemp Oncol ， 2016 ， 3 ： 199 - 204 . DOI： 10.5114/wo.2016.57814 http://dx.doi.org/10.5114/wo.2016.57814 .

AMER M E ， OTHMAN A I ， ABOZAID H M ， et al . Utility of melatonin in mitigating ionizing radiation-induced testis injury through synergistic interdependence of its biological properties ［J］. Biol Res ， 2022 ， 55 （ 1 ）： 33 . DOI： 10.1186/s40659-022-00401-6 http://dx.doi.org/10.1186/s40659-022-00401-6 .

YANG W Q ， NONG W H ， LIU K ， et al . Nicotinamide mononucleotide ameliorates ionizing radiation-induced spermatogenic dysfunction in mice by modulating the glycolytic pathway ［J］. Acta Biochim Biophys Sin ， 2024 ， 57 （ 2 ）： 274 - 285 . DOI： 10.3724/abbs.2024167 http://dx.doi.org/10.3724/abbs.2024167 .

ZHANG Y F ， DENG H X ， YANG Z Q ， et al . Treatment of radiation bone injury with transplanted hUCB-MSCs via Wnt/β-catenin ［J］. Stem Cells Int ， 2021 ， 2021 ： 5660927 . DOI： 10.1155/2021/5660927 http://dx.doi.org/10.1155/2021/5660927 .

张鑫辉，牛世英，姚树桐，等 . BMSC促进巨噬细胞M2型极化减轻急性放射性肺损伤［J］. 中国辐射卫生， 2024 ， 33 （ 1 ）： 21 - 27 . DOI： 10.13491/j.issn.1004-714X.2024.01.004 http://dx.doi.org/10.13491/j.issn.1004-714X.2024.01.004 .

ZHANG X H ， NIU S Y ， YAO S T ， et al . BMSCs promote M2 macrophage polarization to attenuate acute radiation-induced lung injury ［J］. Chin J Radiol Health ， 2024 ， 33 （ 1 ）： 21 - 27 . DOI： 10.13491/j.issn.1004-714X.2024.01.004 http://dx.doi.org/10.13491/j.issn.1004-714X.2024.01.004 .

ABDELAZIZ M H ， SALAH EL-DIN E Y ， EL-DAKDOKY M H ， et al . The impact of mesenchymal stem cells on doxorubicin-induced testicular toxicity and progeny outcome of male prepubertal rats ［J］. Birth Defects Res ， 2019 ， 111 （ 13 ）： 906 - 919 . DOI： 10.1002/bdr2.1535 http://dx.doi.org/10.1002/bdr2.1535 .

ZHANG Y S ， LIU Y N ， TENG Z ， et al . Human umbilical cord mesenchymal stem cells （hUC-MSCs） alleviate paclitaxel-induced spermatogenesis defects and maintain male fertility ［J］. Biol Res ， 2023 ， 56 （ 1 ）： 47 . DOI： 10.1186/s40659-023-00459-w http://dx.doi.org/10.1186/s40659-023-00459-w .

ZHAO S S ， LI Z Z ， LI K ， et al . Repairing effect of mesenchymal stem cells on lead acetate-induced testicular injury in mice ［J］. Cell Transplant ， 2024 ， 33 ： 09636897231219395 . DOI： 10.1177/09636897231219395 http://dx.doi.org/10.1177/09636897231219395 .

MEHRA B L ， SKANDHAN K P ， PRASAD B S ， et al . Male infertility rate： a retrospective study ［J］. Urologia ， 2018 ， 85 （ 1 ）： 22 - 24 . DOI： 10.5301/uj.5000254 http://dx.doi.org/10.5301/uj.5000254 .

SANDERS J E ， HAWLEY J ， LEVY W ， et al . Pregnancies following high-dose cyclophosphamide with or without high-dose busulfan or total-body irradiation and bone marrow transplantation ［J］. Blood ， 1996 ， 87 （ 7 ）： 3045 - 3052 .

VERGOUWEN R P ， HUISKAMP R ， BAS R J ， et al . Radiosensitivity of testicular cells in the prepubertal mouse ［J］. Radiat Res ， 1994 ， 139 （ 3 ）： 316 - 326 .

KHAN S ， ADHIKARI J S ， RIZVI M A ， et al . Radioprotective potential of melatonin against 60 Co γ-ray-induced testicular injury in male C57BL/6 mice ［J］. J Biomed Sci ， 2015 ， 22 （ 1 ）： 61 . DOI： 10.1186/s12929-015-0156-9 http://dx.doi.org/10.1186/s12929-015-0156-9 .

QU N ， ITOH M ， SAKABE K . Effects of chemotherapy and radiotherapy on spermatogenesis： the role of testicular immunology ［J］. Int J Mol Sci ， 2019 ， 20 （ 4 ）： 957 . DOI： 10.3390/ijms20040957 http://dx.doi.org/10.3390/ijms20040957 .

马秦龙，林敏，高鹏，等 . 2.5 T太赫兹辐射暴露对小鼠睾丸组织损伤效应及机制研究［J］. 陆军军医大学学报， 2024 ， 46 （ 19 ）： 2197 - 2207 . DOI： 10.16016/j.2097-0927.202311151 http://dx.doi.org/10.16016/j.2097-0927.202311151 .

MA Q L ， LIN M ， GAO P ， et al . Effects and mechanisms of 2.5T terahertz radiation exposure on testis injury in mice ［J］. J Army Med Univ ， 2024 ， 46 （ 19 ）： 2197 - 2207 . DOI： 10.16016/j.2097-0927.202311151 http://dx.doi.org/10.16016/j.2097-0927.202311151 .

EHGHAGHI A ， ZOKAEI E ， MODARRESSI M H ， et al . Antioxidant and anti-apoptotic effects of selenium nanoparticles and Lactobacillus casei on mice testis afte r X-ray ［J］. Andrologia ， 2022 ， 54 （ 11 ）： e14591 . DOI： 10.1111/and.14591 http://dx.doi.org/10.1111/and.14591 .

SAID R S ， MOHAMED H A ， KAMAL M M . Coenzyme Q10 mitigates ionizing radiation-induced testicular damage in rats through inhibition of oxidative stress and mitochondria-mediated apoptotic cell death ［J］. Toxicol Appl Pharmacol ， 2019 ， 383 ： 114780 . DOI： 10.1016/j.taap.2019.114780 http://dx.doi.org/10.1016/j.taap.2019.114780 .

GUILLAMAT-PRATS R . The role of MSC in wound healing， scarring and regeneration ［J］. Cells ， 2021 ， 10 （ 7 ）： 1729 . DOI： 10.3390/cells10071729 http://dx.doi.org/10.3390/cells10071729 .

WDOWIAK A ， SKRZYPEK M ， STEC M ， et al . Effect of ionizing radiation on the male reproductive system ［J］. Ann Agric Environ Med ， 2019 ， 26 （ 2 ）： 210 - 216 . DOI： 10.26444/aaem/106085 http://dx.doi.org/10.26444/aaem/106085 .

KESARI K K ， AGARWAL A ， HENKEL R . Radiations and male fertility ［J］. Reprod Biol Endocrinol ， 2018 ， 16 ： 118 . DOI： 10.1186/s12958-018-0431-1 http://dx.doi.org/10.1186/s12958-018-0431-1 .

AL TURKI H A ， AL-SUHAIBANI S S ， ALSHAMLAN D Y ， et al . Autologous bone marrow-derived mesenchymal stem cells in the reversal of unobstructed azoospermia in rats ［J］. Stem Cells Cloning ， 2024 ， 17 ： 33 - 39 . DOI： 10.2147/SCCAA.S481267 http://dx.doi.org/10.2147/SCCAA.S481267 .

CETINKAYA-UN B ， UN B ， AKPOLAT M ， et al . Human amnion membrane-derived mesenchymal stem cells and conditioned medium can ameliorate X-irradiation-induced testicular injury by reducing endoplasmic reticulum stress and apoptosis ［J］. Reprod Sci ， 2022 ， 29 （ 3 ）： 944 - 954 . DOI： 10.1007/s43032-021-00753-6 http://dx.doi.org/10.1007/s43032-021-00753-6 .

WANG Y J ， YAN J ， ZOU X L ， et al . Bone marrow mesenchymal stem cells repair cadmium-induced rat testis injury by inhibiting mitochondrial apoptosis ［J］. Chem Biol Interact ， 2017 ， 271 ： 39 - 47 . DOI： 10.1016/j.cbi. 2017. 04.024 http://dx.doi.org/10.1016/j.cbi.2017.04.024 .

ELMHIRI G ， GLOAGUEN C ， GRISON S ， et al . DNA methylation and potential multigenerational epigenetic effects linked to uranium chronic low-dose exposure in gonads of males and females rats ［J］. Toxicol Lett ， 2018 ， 282 ： 64 - 70 . DOI： 10.1016/j.toxlet.2017.10.004 http://dx.doi.org/10.1016/j.toxlet.2017.10.004 .

LIU G W ， GONG P S ， ZHAO H G ， et al . Effect of low-level radiation on the death of male germ cells ［J］. Radiat Res ， 2006 ， 165 （ 4 ）： 379 - 389 . DOI： 10.1667/rr3528.1 http://dx.doi.org/10.1667/rr3528.1 .

ASADI A ， GHAHREMANI R ， ABDOLMALEKI A ， et al . Role of sperm apoptosis and oxidative stress in male infertility： a narrative review ［J］. Int J Reprod Biomed ， 2021 ， 19 （ 6 ）： 493 - 504 . DOI： 10.18502/ijrm.v19i6.9371 http://dx.doi.org/10.18502/ijrm.v19i6.9371 .

PARK Y J ， PANG M G . Mitochondrial functionality in male fertility： from spermatogenesis to fertilization ［J］. Antioxidants ， 2021 ， 10 （ 1 ）： 98 . DOI： 10.3390/antiox 10010098 http://dx.doi.org/10.3390/antiox10010098 .

DURAIRAJANAYAGAM D ， SINGH D ， AGARWAL A ， et al . Causes and consequences of sperm mitochondrial dysfunction ［J］. Andrologia ， 2021 ， 53 （ 1 ）： e13666 . DOI： 10.1111/and.13666 http://dx.doi.org/10.1111/and.13666 .

MEYER J N ， LEUTHNER T C ， LUZ A L . Mitochondrial fusion， fission， and mitochondrial toxicity ［J］. Toxicology ， 2017 ， 391 ： 42 - 53 . DOI： 10.1016/j.tox.2017.07.019 http://dx.doi.org/10.1016/j.tox.2017.07.019 .

ANDERSON G R ， WARDELL S E ， CAKIR M ， et al . Dysregulation of mitochondrial dynamics proteins are a targetable feature of human tumors ［J］. Nat Commun ， 2018 ， 9 （ 1 ）： 1677 . DOI： 10.1038/s41467-018-04033-x http://dx.doi.org/10.1038/s41467-018-04033-x .

CAO Y H ， CHEN X ， PAN F Q ， et al . Xinmaikang-mediated mitophagy attenuates atherosclerosis via the PINK1/Parkin signaling pathway ［J］. Phytomedicine ， 2023 ， 119 ： 154955 . DOI： 10.1016/j.phymed.2023.154955 http://dx.doi.org/10.1016/j.phymed.2023.154955 .

KNUDSON C M ， TUNG K S ， TOURTELLOTTE W G ， et al . Bax-deficient mice with lymphoid hyperplasia and male germ cell death ［J］. Science ， 1995 ， 270 （ 5233 ）： 96 - 99 . DOI： 10.1126/science.270.5233.96 http://dx.doi.org/10.1126/science.270.5233.96 .

CAO X B ， WEN P B ， FU Y F ， et al . Radiation induces apoptosis primarily through the intrinsic pathway in mammalian cells ［J］. Cell Signal ， 2019 ， 62 ： 109337 . DOI： 10.1016/j.cellsig.2019.06.002 http://dx.doi.org/10.1016/j.cellsig.2019.06.002 .

ZHANG J ， NEY P A . Role of BNIP3 and NIX in cell death， autophagy， and mitophagy ［J］. Cell Death Differ ， 2009 ， 16 （ 7 ）： 939 - 946 . DOI： 10.1038/cdd.2009.16 http://dx.doi.org/10.1038/cdd.2009.16 .

ZHOU J L ， YAO W ， LI C Y ， et al . Administration of follicle-stimulating hormone induces autophagy via upregulation of HIF-1α in mouse granulosa cells ［J］. Cell Death Dis ， 2017 ， 8 （ 8 ）： e3001 . DOI： 10.1038/cddis. 2017.371 http://dx.doi.org/10.1038/cddis.2017.371 .

CHEN H ， TANG Q L ， WU X Y ， et al . Differentiation of human umbilical cord mesenchymal stem cells into germ-like cells in mouse seminiferous tubules ［J］. Mol Med Rep ， 2015 ， 12 （ 1 ）： 819 - 828 . DOI： 10.3892/mmr.2015.3528 http://dx.doi.org/10.3892/mmr.2015.3528 .

YANG R F ， LIU T H ， ZHAO K ， et al . Enhancement of mouse germ cell-associated genes expression by injection of human umbilical cord mesenchymal stem cells into the testis of chemical-induced azoospermic mice ［J］. Asian J Androl ， 2014 ， 16 （ 5 ）： 698 - 704 . DOI： 10.4103/1008-682X.129209 http://dx.doi.org/10.4103/1008-682X.129209 .

SAGARADZE G D ， BASALOVA N A ， KIRPATOVSKY V I ， et al . Application of rat cryptorchidism model for the evaluation of mesenchymal stromal cell secretome regenerative potential ［J］. Biomed Pharmacother ， 2019 ， 109 ： 1428 - 1436 . DOI： 10.1016/j.biopha.2018.10.174 http://dx.doi.org/10.1016/j.biopha.2018.10.174 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Roxadustat attenuates brain injury in mice with heat stroke by regulating mitochondrial fission and fusion

Effects of esmolol on cardiomyocyte apoptosis in septic rats

Protective effect of equol on mitochondrial damage induced by high glucose and fat in osteoblasts

Bone marrow-derived mesenchymal stem cells alleviate inflammation and tissue injury in severe acute pancreatitis by suppressing NLRP3/GSDMD-mediated pyroptosis

CXCL12 promotes endometrial repair through MSC homing in a mouse model of intrauterine adhesions: Multimechanistic study based on angiogenesis, cell proliferation and Sirt1 upregulation

Related Author

Huan ZHOU

Xueyan HUANG

Tingting SHEN

Boyi ZHANG

Genlin HE

Xue LUO

Xuesen YANG

Fei SI

Related Institution

Department of Tropical Medicine, Key Laboratory of Extreme Environmental Medicine of Ministry of Education, Faculty of Military Preventive Medicine, Army Medical University (Third Military Medical University)

Second Clinical Medical College of Lanzhou University

Ward 3 of Department of Critical Care Medicine, Lanzhou University Second Hospital

Department of Gastroenterology， Shanghai Municipal Hospital of Traditional Chinese Medicine Affiliated to Shanghai University of Traditional Chinese Medicine

Department of Gastroenterology， the First Affiliated Hospital of Naval Medical University

⁰

Transplantation of human umbilical cord mesenchymal stem cells repairs radiation-induced reproductive injury in male mice via improving mitochondrial function and attenuating oxidative stress

DOI：10.16016/j.2097-0927.202510053

摘要

Abstract

关键词

Keywords

references