Effects of LncRNA FGD5-AS1 on migration and invasion of gastric cancer cells by regulating miR-302b-3p/E2F1 axis

LI Na; LI Hao; LIN Kun; HE Yanxin; ZHENG Yinglan

doi:10.16016/j.2097-0927.202510027

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Effects of LncRNA FGD5-AS1 on migration and invasion of gastric cancer cells by regulating miR-302b-3p/E2F1 axis

Basic Medicine | 更新时间：2025-12-30

- Effects of LncRNA FGD5-AS1 on migration and invasion of gastric cancer cells by regulating miR-302b-3p/E2F1 axis
- Journal of Army Medical University Vol. 47, Issue 24, Pages: 3065-3076(2025)
- 作者机构：
  
  1.康复大学青岛中心医院，消化内科，山东青岛
  2.康复大学青岛中心医院，内镜中心，山东青岛
- 作者简介：
  
  ZHENG Yinglan，E-mail：wtsgnc@163.com
- 基金信息：
  
  the Medical Science Research Guidance Project of Qingdao City in 2023(2023-WJZD189)
- DOI：10.16016/j.2097-0927.202510027
  CLC： R394.3;R730.23;R735.2
- Received：14 October 2025，
  
  Revised：2025-11-28，
  
  Published：30 December 2025
- 稿件说明：
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李娜，李浩，林坤，等.LncRNA FGD5-AS1通过调节miR-302b-3p/E2F1轴对胃癌细胞迁移及侵袭的影响［J］.陆军军医大学学报，2025，47（24）：3065-3076.DOI：10.16016/j.2097-0927.202510027.

LI N，LI H，LIN K，et al.Effects of LncRNA FGD5-AS1 on migration and invasion of gastric cancer cells by regulating miR-302b-3p/E2F1 axis［J］.J Army Med Univ，2025，47（24）：3065-3076.DOI：10.16016/j.2097-0927.202510027.
李娜，李浩，林坤，等.LncRNA FGD5-AS1通过调节miR-302b-3p/E2F1轴对胃癌细胞迁移及侵袭的影响［J］.陆军军医大学学报，2025，47（24）：3065-3076.DOI：10.16016/j.2097-0927.202510027. DOI：

LI N，LI H，LIN K，et al.Effects of LncRNA FGD5-AS1 on migration and invasion of gastric cancer cells by regulating miR-302b-3p/E2F1 axis［J］.J Army Med Univ，2025，47（24）：3065-3076.DOI：10.16016/j.2097-0927.202510027. DOI：

摘要

目的

探讨长链非编码RNA FGD5-AS1（LncRNA FGD5-AS1）调节微小RNA-302b-3p（miR-302b-3p）/E2F转录因子1（E2F1）轴对胃癌细胞迁移及侵袭的影响。

方法

实时荧光定量PCR反应（qRT-PCR）检测胃癌细胞系（SGC-7901、MKN-45、HGC-27、AGS、BGC-823）及人胃粘膜上皮细胞系GES-1中LncRNA FGD5-AS1、miR-302b-3p、E2F1 mRNA表达，筛选最佳干预细胞。将胃癌细胞分为对照组（NC组）、sh-NC组、sh-FGD5-AS1组、sh-FGD5-AS1+anti-miR-NC组、sh-FGD5-AS1+anti-miR-302b-3p组。qRT-PCR检测细胞中LncRNA FGD5-AS1、miR-302b-3p、E2F1 mRNA的表达水平；MTT法及流式细胞仪分别检测细胞增殖与凋亡；划痕实验及Transwell实验分别检测细胞迁移与侵袭；Western blot检测E2F1、Ki-67、Bax、Bcl-2蛋白表达；裸鼠移植瘤实验验证LncRNA FGD5-AS1对胃癌移植瘤生长的影响；双荧光素酶报告基因和RNA pull-down实验检测LncRNA FGD5-AS1、E2F1与miR-302b-3p的靶向关系。

结果

与GES-1相比，胃癌细胞系（SGC-7901、MKN-45、HGC-27、AGS、BGC-823）中LncRNA FGD5-AS1、E2F1 mRNA表达水平升高，miR-302b-3p表达水平降低（

0.05），选择AGS细胞进行实验；沉默LncRNA FGD5-AS1表达可显著上调miR-302b-3p表达，下调E2F1表达，抑制胃癌细胞增殖、迁移与侵袭，促进细胞凋亡（

0.05）；抑制miR-

302b-3p表达可部分减弱沉默LncRNA FGD5-AS1对胃癌细胞增殖、迁移、侵袭及凋亡的作用（

0.05）；体内实验显示，沉默LncRNA FGD5-AS1表达可显著抑制胃癌移植瘤小鼠肿瘤的生长（

0.05）；RNA pull-down实验、双荧光素酶报告基因实验证实LncRNA FGD5-AS1、E2F1与miR-302b-3p存在靶向调控关系。

结论

LncRNA FGD5-AS1在胃癌细胞中上调表达，沉默LncRNA FGD5-AS1表达可通过调节miR-302b-3p/E2F1轴，抑制胃癌恶性进展。

Abstract

Objective

To explore the effects of long non coding RNA FGD5-AS1 (LncRNA FGD5-AS1) on the migration and invasion of gastric cancer cells by regulating the microRNA-302b-3p (miR-302b-3p)/E2F transcription factor 1 (E2F1) axis.

Methods

Real-time fluorescence quantitative PCR (qRT-PCR) was used to detect the mRNA expression of LncRNA FGD5-AS1

miR-302b-3p

and E2F1 in gastric cancer cell lines (SGC-7901

MKN-45

HGC-27

AGS

BGC-823) and human gastric epithelial cell line GES-1

in order to screen for the best intervention cells. Gastric cancer cells were separated into control group (NC group)

sh-NC group

sh-FGD5-AS1 group

sh-FGD5-AS1+anti-miR-NC group

and sh-FGD5-AS1+anti-miR-302b-3p group. qRT-PCR was performed to detect the expression of LncRNA FGD5-AS1

miR-302b-3p

and E2F1 in the cells. MTT assay and flow cytometry were applied to measure cell proliferation and apoptosis

respectively. Scratch and Transwell assays were carried out to measure cell migration and invasion

respectively. Western blotting was carried out to measure the protein levels of E2F1

Ki-67

Bax and Bcl-2. Nude mouse model of transplantable subcutaneous tumor was established to verify the effect of LncRNA FGD5-AS1 on the growth of gastric cancer transplant tumors. Dual luciferase reporter gene and RNA pull-down assays were used to detect the targeting relationship of LncRNA FGD5-AS1 and E2F1 with miR-302b-3p.

Results

Compared with the GES-1 cells

the gastric cancer cell lines SGC-7901

MKN-45

HGC-27

AGS and BGC-823 exhibited significantly increase

d mRNA levels of LncRNA FGD5-AS1 and E2F1

while decreased level of miR-302b-3p (

0.05). AGS cells were finally selected for the following experiments. Silencing LncRNA FGD5-AS1 prominently up-regulated miR-302b-3p

down-regulated E2F1

and inhibited cell proliferation

migration

and invasion

and promoted cell apoptosis in gastric cancer AGS cells (

0.05). Inhibition of miR-302b-3p partially weakened the effects of silencing LncRNA FGD5-AS1 on the proliferation

migration

invasion

and apoptosis of gastric cancer cells (

0.05).

In vivo

experiments showed that silencing LncRNA FGD5-AS1 could prominently inhibit the growth of transplanted tumors in mice (

0.05). RNA pull-down and dual luciferase reporter gene assays confirmed the targeted regulatory relationship of LncRNA FGD5-AS1 and E2F1 with miR-302b-3p.

Conclusion

LncRNA FGD5-AS1 is up-regulated in gastric cancer cells

and its silencing can inhibit the malignant progression of gastric cancer by regulating the miR-302b-3p/E2F1 axis.

关键词

Keywords

references

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