Expression of β-arrestin1 in oral squamous cell carcinoma and its effect on cell proliferation, migration and invasion

HAO Xiaohui; CHEN Min; WU Nan; DING Yunshan; ZHU Lifan; DAI Haitao

doi:10.16016/j.2097-0927.202504071

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Expression of β-arrestin1 in oral squamous cell carcinoma and its effect on cell proliferation, migration and invasion

Basic Medicine | 更新时间：2025-07-25

- Expression of β-arrestin1 in oral squamous cell carcinoma and its effect on cell proliferation, migration and invasion
- Journal of Army Medical University Vol. 47, Issue 14, Pages: 1632-1641(2025)
- 作者机构：
  
  1.石河子大学医学院，新疆石河子
  2.石河子大学第一附属医院口腔科，新疆石河子
- 作者简介：
  
  DAI Haitao， E-mail： 48440045@qq.com
- 基金信息：
  
  the Project of Science and Technology Plan of Xinjiang Production and Construction Corps(2024ZD067);the Scientific Research Project of the First Affiliated Hospital of Shihezi University(LC2023005);the Innovation Project of Resident and Postgraduate Students in the First Affiliated Hospital of Shihezi University(ZP2023083)
- DOI：10.16016/j.2097-0927.202504071
  CLC： R341;R730.23;R739.85
- Received：18 April 2025，
  
  Revised：05 July 2025，
  
  Published：30 July 2025
- 稿件说明：
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郝小慧，陈敏，仵楠，等.β-arrestin1在口腔鳞状细胞癌中的表达及其对细胞增殖、迁移、侵袭的作用［J］.陆军军医大学学报，2025，47（14）：1632-1641.DOI：10.16016/j.2097-0927.202504071.

HAO X H，CHEN M，WU N，et al.Expression of β-arrestin1 in oral squamous cell carcinoma and its effect on cell proliferation， migration and invasion［J］.J Army Med Univ，2025，47（14）：1632-1641.DOI：10.16016/j.2097-0927.202504071.
郝小慧，陈敏，仵楠，等.β-arrestin1在口腔鳞状细胞癌中的表达及其对细胞增殖、迁移、侵袭的作用［J］.陆军军医大学学报，2025，47（14）：1632-1641.DOI：10.16016/j.2097-0927.202504071. DOI：

HAO X H，CHEN M，WU N，et al.Expression of β-arrestin1 in oral squamous cell carcinoma and its effect on cell proliferation， migration and invasion［J］.J Army Med Univ，2025，47（14）：1632-1641.DOI：10.16016/j.2097-0927.202504071. DOI：

摘要

目的

探讨β-arrestin1（ARRB1）在口腔鳞状细胞癌（oral squamous cell carcinoma，OSCC）中对细胞增殖、迁移和侵袭的作用。

方法

基于TCGA数据库分析ARRB1在OSCC中的表达特征，并利用GSEA富集分析表明其可能涉及的信号通路，同时探讨其对OSCC患者预后的潜在影响。进一步通过免疫组化染色评估ARRB1在癌及癌旁组织中的表达差异，结合统计分析ARRB1与临床病理特征的相关性。利用qPCR和Western blot验证ARRB1在SCC-15、CAL-27及HOK中的表达特征。构建ARRB1过表达质粒模型，结合克隆形成、EdU、划痕、Transwell实验分析其对OSCC细胞增殖、迁移和侵袭的影响。

结果

TCGA分析显示，ARRB1在HNSC和OSCC组织中的表达水平显著低于正常组织（

0.01）。ARRB1在OSCC组织中的表达与肿瘤分化程度、淋巴结转移及TNM分期相关（

0.05）。高表达ARRB1的患者生存率较低表达组高（

=0.50，

=0.041），这与生信分析结果一致。经实验验证，SCC-15、CAL-27中ARRB1表达水平低于HOK（

0.01），且ARRB1过表达显著抑制细胞增殖（

0.05）、迁移（

0.01）和侵袭（

0.01）。

结论

ARRB1在OSCC中低表达，过表达ARRB1抑制OSCC细胞增殖、迁移和侵袭，并与患者预后改善相关。

Abstract

Objective‍　‍To investigate the effect of β-arrestin1 (ARRB1) on cell proliferation

migration and invasion in oral squamous cell carcinoma (OSCC). Methods‍　‍Based on The Cancer Genome Atlas (TCGA) database

the expression profiles of ARRB1 in OSCC were analyzed

and then Gene Set Enrichment Analysis (GSEA) was used to suggest the possible signaling pathways involved

and to explore its potential impact on the prognosis of OSCC patients. Immuinohistochemistry (IHC) was performed to detect the expression of ARRB1 in OSCC tumor tissues and adjacent tissues

and the correlation between ARRB1 expression and clinicopathological features was statistically analyzed. The expression profiles of ARRB1 in SCC-15

CAL-27 and HOK cell lines were verified by qPCR and Western blotting. The ARRB1 overexpression plasmid model was constructed

and its effects on the proliferation

migration and invasion of OSCC cells were analyzed by clone formation

EdU

scratch and Transwell assays. Results‍　‍TCGA showed that the expression level of ARRB1 was significantly lower in head and neck squamous cell carcinoma (HNSC) and OSCC tissues than the corresponding normal tissues (

0.01). The expression of ARRB1 in OSCC tissues was correlated with tumor differentiation

lymph node metastasis and TNM stage (

0.05). The OSCC patients with high expression of ARRB1 had a lower survival rate than those with low expression (

0.01)

which was consistent with the results of bioinformatics analysis. The expression level of ARRB1 in SCC-15 and CAL-27 cells was lower than that of HOK cells (

0.01)

and its overexpression significantly inhibited cell proliferation (

0.05)

migra

tion (

0.01) and invasion (

0.01). Conclusion‍　‍ARRB1 is lowly expressed in OSCC

its overexpression inhibits the proliferation

migration and invasion of OSCC cells

and it is related to prognosis improvement.

关键词

Keywords

references

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