Intestinal blast injuries after different intensity waveshock in rats exposure to extremely cold environment: a comparative study

YUE Yongchao; ZHANG Libin; ZHOU Wenqi; WANG Junren; WU Pengfei; WU Guangyan; MA Hai

doi:10.16016/j.2097-0927.202504066

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Intestinal blast injuries after different intensity waveshock in rats exposure to extremely cold environment: a comparative study

Military Medicine | 更新时间：2025-09-11

- Intestinal blast injuries after different intensity waveshock in rats exposure to extremely cold environment: a comparative study
- Journal of Army Medical University Vol. 47, Issue 17, Pages: 2071-2078(2025)
- 作者机构：
  
  1.陆军第948医院普通外科，新疆维吾尔自治区塔城地区乌苏市
  2.陆军特色医学中心（第三军医大学大坪医院）交通医学研究所，重庆
  3.陆军军医大学（第三军医大学）基础医学院教学实验中心，重庆
- 作者简介：
  
  MA Hai， E-mail： 13649988188@163.com
- 基金信息：
  
  the Special Fund for Key Specialties
- DOI：10.16016/j.2097-0927.202504066
  CLC： R339.55;R642;R656.9
- Received：16 April 2025，
  
  Revised：2025-07-05，
  
  Accepted：07 July 2025，
  
  Published：15 September 2025
- 稿件说明：
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岳永超，章利彬，周文奇，等.高寒环境暴露后大鼠不同强度肠道冲击伤的比较研究［J］.陆军军医大学学报，2025，47（17）：2071-2078.DOI：10.16016/j.2097-0927.202504066.

YUE Y C，ZHANG L B，ZHOU W Q，et al.Intestinal blast injuries after different intensity waveshock in rats exposure to extremely cold environment： a comparative study［J］.J Army Med Univ，2025，47（17）：2071-2078.DOI：10.16016/j.2097-0927.202504066.
岳永超，章利彬，周文奇，等.高寒环境暴露后大鼠不同强度肠道冲击伤的比较研究［J］.陆军军医大学学报，2025，47（17）：2071-2078.DOI：10.16016/j.2097-0927.202504066. DOI：

YUE Y C，ZHANG L B，ZHOU W Q，et al.Intestinal blast injuries after different intensity waveshock in rats exposure to extremely cold environment： a comparative study［J］.J Army Med Univ，2025，47（17）：2071-2078.DOI：10.16016/j.2097-0927.202504066. DOI：

摘要

目的

对高寒环境暴露后大鼠的不同强度肠道冲击伤进行比较，并对其损伤特点进行初步探究。

方法

选取体质量为200~250 g的2月龄健康雄性SD大鼠60只，按随机数字表法将大鼠分为6组（

=10）：空白对照组、低温对照组、5.0 MPa冲击对照组、低温+4.0 MPa冲击组、低温+4.5 MPa冲击组和低温+5.0 MPa冲击组。低温+冲击组在-10 ℃低温环境预处理30 min后，立即采用BST-Ⅰ型生物激波管以不同驱动压冲击大鼠腹部致肠道损伤。检测致伤后3、8、24 h大鼠血清白介素-6（interleukin-6，IL-6）、肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）、肠脂肪酸结合蛋白（intestinal-fatty acid binding protein， I-FABP）、二胺氧化酶（diamine oxidase， DAO）水平，并记录大鼠24 h存活情况。实验24 h后，麻醉处死大鼠并解剖，观察肠道损伤特点并行病理学检测。通过比较各组差异，研究高寒环境暴露后大鼠不同强度肠道冲击伤特点。

结果

较空白对照组，其余各组大鼠均出现不同程度肠道损伤，且低温+冲击伤组大鼠肠道更易出现水肿及损伤，低温+5.0 MPa冲击组存活率明显下降（

0.05）。病理学及血清学研究发现，低温+冲击组大鼠肠黏膜在高寒环境和冲击伤双重作用下，表现出肠黏膜出血、水肿、黏膜固有层崩解等；肠黏膜损伤指标及肠道炎症因子也较空白对照组明显升高，且随冲击强度增加，组间差异亦有统计学意义（

0.05）。

结论

低温暴露叠加腹部冲击波损伤后，大鼠死亡率明显增加，肠道损伤表现为血清学指标及肠道炎症因子升高，不同程度的肠壁水肿、黏膜下出血等，且损伤严重程度与冲击强度相关，随冲击强度增加而加重。

Abstract

Objective‍　‍To compare the intestinal injury induced by different intensities of waveshock in rats exposed to extremely cold environment and to preliminarily explore the characteristics of the injury. Methods‍　‍Sixty healthy male SD rats (2 months old

weighing 200~250 g) were randomly divided into 6 groups(

=10): blank control group

low-temperature control group

5.0 MPa shock control group

and low-temperature+4.0

4.5 and 5.0 MPa shock groups. The rats in the experimental groups were pre-treated in a -10 ℃ low-temperature environment for 30 min and then subjected to intestinal injury by using BST-I biological shock tube with different driving pressures. At 3

and 24 h after injury

the serum levels of IL-6

TNF-α

intestinal fatty acid binding protein (I-FABP)

and diamine oxidase (DAO) were detected

and the survival of rats within 24 h was recorded. At 24 h after injury

the rats were anesthetized and dissected

the characteristics of intestinal injury were observed

and pathological examination was performed. The di

fferences of intestinal injury were compared among the 6 groups to explore the characteristics of intestinal injuries after different intensities of shockwave in rats after exposure to extremely cold environment. Results‍　‍Compared with the blank control group

the other 5 groups exhibited different severities of intestinal injury

and the rats in the low-temperature+ different shock groups were more prone to intestinal edema and trauma. The mortality rate was significantly increased in the low-temperature+5.0 MPa shock group (

0.05). Pathological and serological studies found that dual effects of very cold environment and blast injury resulted in intestinal mucosal hemorrhage

edema

and disintegration of lamina propria in the experimental rats. The indicators of intestinal mucosal injury and intestinal inflammatory factors were also significantly increased when compared with the blank control group

and significant differences were among the groups with increment of shock intensity (

0.05). Conclusion‍　‍Exposure to very cold environment combined with abdominal blast injury increases mortality rate in rats

manifested by elevated serological indicators and intestinal inflammatory factors

as well as varying severities of intestinal wall edema and submucosal bleeding. Furthermore

the severity of the injury is positively related to the impact intensity

with worsened as the impact intensity increasing.

关键词

Keywords

references

王正国 . 原发肺冲击伤［J/OL］. 中华肺部疾病杂志（电子版）， 2010 ， 3 （ 4 ）： 231 - 233 .

WANG Z G . Primary blast lung injury ［J/OL］. Chin J Lung Dis Electron Ed ， 2010 ， 3 （ 4 ）： 231 - 233 .

王正国，蒋建新 . 爆炸冲击伤原理与实践［M］. 北京：人民卫生出版社， 2020 .

WANG Z G . JIANG J X . Principles and Practice of Blast Shock Injury ［M］. Beijing ： People’s Medical Publishing House ， 2020 .

赵松韵，刁朝玥，白冲 . 原发性肺冲击伤相关信号通路研究进展［J］. 海军军医大学学报， 2025 ， 46 （ 3 ）： 406 - 410 . DOI： 10.16781/j.CN31-2187/R.20220452 http://dx.doi.org/10.16781/j.CN31-2187/R.20220452 .

ZHAO S Y ， DIAO C Y ， BAI C . Signaling pathways related to primary blast lung injury： research progress ［J］. Acad J Nav Med Univ ， 2025 ， 46 （ 3 ）： 406 - 410 . DOI： 10.16781/j.CN31-2187/R.20220452 http://dx.doi.org/10.16781/j.CN31-2187/R.20220452 .

TURÉGANO-FUENTES F ， PÉREZ-DIAZ D ， SANZ-SÁNCHEZ M ， et al . Abdominal blast injuries： different patterns， severity， management， and prognosis according to the main mechanism of injury ［J］. Eur J Trauma Emerg Surg ， 2014 ， 40 （ 4 ）： 451 - 460 . DOI： 10.1007/s00068-014-0397-4 http://dx.doi.org/10.1007/s00068-014-0397-4 .

CHU P ， LI Y F ， HAN X M ， et al . Mechanism of low temperature-induced intestinal damage in Danio rerio and the mitigating effect of alanylglutamine ［J］. Water Biol Secur ， 2025 ： 100377 . DOI： 10.1016/j.watbs.2025.100377 http://dx.doi.org/10.1016/j.watbs.2025.100377 .

张仕忠，李进平，康越，等 . 激波管模拟产生近场爆炸冲击波［J］. 爆炸与冲击， 2024 ， 44 （ 12 ）： 137 - 148 .

ZHANG S Z ， LI J P ， KANG Y ， et al . Generation of near-field blast wave by means of shock tube ［J］. Explos Shock Waves ， 2024 ， 44 （ 12 ）： 137 - 148 .

LAI H J ， ZHAN Y Q ， QIU Y X ， et al . HMGB1 signaling-regulated endoplasmic reticulum stress mediates intestinal ischemia/reperfusion-induced acute renal damage ［J］. Surgery ， 2021 ， 170 （ 1 ）： 239 - 248 . DOI： 10.1016/j.surg.2021.01.042 http://dx.doi.org/10.1016/j.surg.2021.01.042 .

胡金树，杨国涛，尹勇，等 . 血清二胺氧化酶活性测定对判断肠缺血患者预后的应用价值［J］. 中国微生态学杂志， 2024 ， 36 （ 3 ）： 298 - 300， 306 . DOI： 10.13381/j.cnki.cjm.202403007 http://dx.doi.org/10.13381/j.cnki.cjm.202403007 .

HU J S ， YANG G T ， YIN Y ， et al . The value of serum diamine oxidase monitoring in predicting the prognosis of patients with intestinal ischemia ［J］. Chin J Microecol ， 2024 ， 36 （ 3 ）： 298 - 300， 306 . DOI： 10.13381/j.cnki.cjm.202403007 http://dx.doi.org/10.13381/j.cnki.cjm.202403007 .

AN J ， LIU Y Q ， WANG Y Q ， et al . The role of intestinal mucosal barrier in autoimmune disease： a potential target ［J］. Front Immunol ， 2022 ， 13 ： 871713 . DOI： 10.3389/fimmu.2022.871713 http://dx.doi.org/10.3389/fimmu.2022.871713 .

SBRAGIA L ， GUALBERTO I J N ， XIA J ， et al . Intestinal fatty acid-binding protein as a marker of necrotizing enterocolitis incidence and severity： a scoping review ［J］. J Surg Res ， 2024 ， 303 ： 613 - 627 . DOI： 10.1016/j.jss.2024. 09.059 http://dx.doi.org/10.1016/j.jss.2024.09.059 .

聂海 . 舱内爆炸致大鼠腹部闭合性损伤的特点及其对肠黏膜屏障功能的影响［D］. 重庆：第三军医大学， 2008 .

NIE H . A pilot study on traumatic characteristics and intestinal mucosal barrier dysfunction associated with closed abdominal injury of rats subjected to explosion in enclosed spaces ［D］. Chongqing ： Third Military Medical University ， 2008 .

KIRIU N ， SAITOH D ， SEKINE Y ， et al . Shock wave damage from the ventral side in primary blast injury： an experimental study in pigs ［J］. Injury ， 2024 ， 55 （ 12 ）： 111982 . DOI： 10.1016/j.injury.2024.111982 http://dx.doi.org/10.1016/j.injury.2024.111982 .

KORKMAZ İ ， ÇELIKKAYA M E . Blast lung injury in children： injury patterns and associated organ injuries ［J］. Pediatr Emerg Care ， 2023 ， 39 （ 9 ）： 715 - 720 . DOI： 10.1097/PEC.0000000000003021 http://dx.doi.org/10.1097/PEC.0000000000003021 .

SCOTT T E ， KIRKMAN E ， HAQUE M ， et al . Primary blast lung injury— a review ［J］. Br J Anaesth ， 2017 ， 118 （ 3 ）： 311 - 316 . DOI： 10.1093/bja/aew385 http://dx.doi.org/10.1093/bja/aew385 .

王玥，严文韬，江雷，等 . 大型恐怖爆炸事件伤情谱特征研究［J］. 创伤外科杂志， 2024 ， 26 （ 09 ）： 647 - 654 . DOI： 10. 3969/j.issn http://dx.doi.org/10.3969/j.issn ， 1009 - 4237 .2024. 09 . 002 .

WANG Y ， YAN W T ， JIANG L ， et al . Injury spectrum of mass casualty terrorist bombing events ［J］. J Trauma Surg ， 2024 ， 26 （ 09 ）： 647 - 654 . DOI： 10.3969/j.issn.1009-4237. 2024.09.002 http://dx.doi.org/10.3969/j.issn.1009-4237.2024.09.002 .

蒋建新，曾灵 . 肺爆炸冲击伤机制与防护研究进展［J］. 陆军军医大学学报， 2022 ， 44 （ 05 ）： 395 - 398 . DOI： 10.16016/j.2097-0927.202111179 http://dx.doi.org/10.16016/j.2097-0927.202111179 .

JIANG J X ， ZENG L . Advance of protection and mechanism of lung blast injury ［J］. J Army Med Univ ， 2022 ， 44 （ 05 ）： 395 - 398 . DOI： 10.16016/j.2097-0927.202111179 http://dx.doi.org/10.16016/j.2097-0927.202111179 .

BOUTILLIER J ， DECK C ， DE MEZZO S ， et al . Lung injury risk assessment during blast exposure ［J］. J Biomech ， 2019 ， 86 ： 210 - 217 . DOI： 10.1016/j.jbiomech.2019.02.011 http://dx.doi.org/10.1016/j.jbiomech.2019.02.011 .

佟昌慈，柳云恩，张玉彪，等 . 高迁移率族蛋白-1、糖基化终产物受体及Toll样受体-4通路对爆震伤致大鼠急性肺损伤影响研究［J］. 创伤与急危重病医学， 2017 ， 5 （ 4 ）： 198 - 204 . DOI： 10.16048/j.issn.2095-5561.2017.04.02 http://dx.doi.org/10.16048/j.issn.2095-5561.2017.04.02 .

TONG C C ， LIU Y E ， ZHANG Y B ， et al . Effects of HMGB-1， RAGE and TLR-4 pathway on acute lung injury induced by blast in rats ［J］. Trauma Crit Care Med ， 2017 ， 5 （ 4 ）： 198 - 204 . DOI： 10.16048/j.issn.2095-5561.2017.04.02 http://dx.doi.org/10.16048/j.issn.2095-5561.2017.04.02 .

喻伯牙，高俊宏 . 不同工况下爆炸冲击波致人员损伤研究进展［J］. 陆军军医大学学报， 2025 ， 47 （ 10 ）： 1029 - 1042 . DOI： 10.16016/j.2097-0927.202406052 http://dx.doi.org/10.16016/j.2097-0927.202406052 .

YU B Y ， GAO J H . Progress in explosive shock wave-caused injuries in complicated work environment ［J］. J Army Med Univ ， 2025 ， 47 （ 10 ）： 1029 - 1042 . DOI： 10.16016/j.2097-0927.202406052 http://dx.doi.org/10.16016/j.2097-0927.202406052 .

GUY R J ， KIRKMAN E ， WATKINS P E ， et al . Physiologic responses to primary blast ［J］. J Trauma ， 1998 . 45 （ 6 ）： 983 - 987 . DOI： 10.1097/00005373-199812000-00001 http://dx.doi.org/10.1097/00005373-199812000-00001 .

何青盈，李悦，文静，等 . RSR13对高原大鼠肺冲击伤的保护作用及机制研究［J］. 陆军军医大学学报， 2024 （ 20 ）： 2352 - 2359 . DOI： 10.16016/j.2097-0927.202404091 http://dx.doi.org/10.16016/j.2097-0927.202404091 .

HE Q Y ， LI Y ， WEN J ， et al . Protective effect and mechanism of RSR13 on lung blast injury in rats at high altitude ［J］. J Army Med Univ ， 2024 （ 20 ）： 2352 - 2359 . DOI： 10.16016/j.2097-0927.202404091 http://dx.doi.org/10.16016/j.2097-0927.202404091 .

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