Erianin inhibits proliferation of activated T cells by downregulating CyclinB1

JING Lin; ZHANG Ting; LUO Xinyan; LIU Yang; LAI Yi

doi:10.16016/j.2097-0927.202503074

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Erianin inhibits proliferation of activated T cells by downregulating CyclinB1

Basic Medicine | 更新时间：2025-10-13

- Erianin inhibits proliferation of activated T cells by downregulating CyclinB1
- Journal of Army Medical University Vol. 47, Issue 19, Pages: 2365-2373(2025)
- 作者机构：
  
  1.广西大学生物医学院，广西特色生物医药重点实验室，广西南宁
  2.成都医学院科研实验中心四川成都
  3.成都医学院检验医学院，四川成都
- 作者简介：
- 基金信息：
  
  the Project of Science and Technology Plan of Sichuan Province(2023NSFSC0624)
- DOI：10.16016/j.2097-0927.202503074
  CLC： R285.5;R331.144;R329.28
- Received：26 March 2025，
  
  Revised：2025-08-12，
  
  Accepted：10 September 2025，
  
  Published：15 October 2025
- 稿件说明：
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敬林，张婷，罗兴燕，等.毛兰素通过下调CyclinB1蛋白表达抑制活化T细胞增殖［J］.陆军军医大学学报，2025，47（19）：2365-2373.DOI：10.16016/j.2097-0927.202503074.

JING L，ZHANG T，LUO X Y，et al.Erianin inhibits proliferation of activated T cells by downregulating CyclinB1［J］.J Army Med Univ，2025，47（19）：2365-2373.DOI：10.16016/j.2097-0927.202503074.
敬林，张婷，罗兴燕，等.毛兰素通过下调CyclinB1蛋白表达抑制活化T细胞增殖［J］.陆军军医大学学报，2025，47（19）：2365-2373.DOI：10.16016/j.2097-0927.202503074. DOI：

JING L，ZHANG T，LUO X Y，et al.Erianin inhibits proliferation of activated T cells by downregulating CyclinB1［J］.J Army Med Univ，2025，47（19）：2365-2373.DOI：10.16016/j.2097-0927.202503074. DOI：

摘要

目的

毛兰素抑制活化T细胞增殖及其作用机制探究。

方法

利用密度梯度离心法分离人外周血单个核细胞（peripheral blood mononuclear cell， PBMC），采用人T细胞MACS磁珠阴性分选试剂盒纯化T细胞，抗人CD3/CD28抗体刺激活化T细胞。采用羧基荧光素琥珀酰亚胺酯（carboxyfluorescein succinimidyl ester，CFSE）标记活化T细胞，通过流式细胞术检测毛兰素对活化T细胞增殖、凋亡、活化标志物CD25表达、细胞周期分布以及静息T细胞存活率的影响。采用酶联免疫吸附测定（enzyme linked immunosorbent assay，ELISA）定量分析毛兰素处理后的活化T细胞培养上清中IL-2和IL-17的分泌水平。Western blot分析毛兰素对细胞分裂周期蛋白2（cell division cycle protein 2，CDC2）、P-CDC2、以及CyclinB1蛋白表达的影响。观察并分析毛兰素处理组（0.05~0.20 μmol/L）与阳性对照组（纯化后仅用抗人CD3/CD28抗体刺激的T细胞）的显著性差异结果。

结果

CFSE增殖结果表明毛兰素处理组（0.05~0.20 μmol/L）的活化T细胞增殖率呈浓度依赖性下降（

0.0001），其半数抑制浓度（IC

）为（0.09±0.10） μmol/L。细胞凋亡和细胞存活率结果表明活化T细胞和静息T细胞经毛兰素处理后，毛兰素处理组与阳性对照组的凋亡率和存活率无显著差异。通过对活化T细胞标志物分析发现，毛兰素不影响CD25的表达和IL-2的分泌。ELISA检测显示毛兰素显著抑制IL-17炎性因子的分泌（

0.0001）。细胞周期实验表明毛兰素阻滞活化T细胞周期于G2/M期（

0.05

）

，进一步机制研究发现，毛兰素不影响P-CDC2、CDC2的表达，但显著抑制CyclinB1蛋白的表达（

0.01）。

结论

毛兰素具有显著免疫抑制活性且通过下调CyclinB1蛋白表达抑制活化T细胞增殖。

Abstract

Objective

To investigate the inhibitory effect of erianin on T cell proliferation and its underlying mechanism.

Methods

Peripheral blood mononuclear cells (PBMCs) were isolated using density gradient centrifugation

and T cells were purified by magnetic-activated cell sorting (MACS) with immunomagnetic beads

followed by being activated with anti-human CD3 antibodies and anti-human CD28 antibodies. The activated T cells were labeled with carboxyfluorescein succinimidyl ester (CFSE)

and the effects of erianin (0.05~0.20 μmol/L) on the proliferation

apoptosis

expression of activation marker cluster of differentiation 25 (CD25)

cell cycle distribution of activated T cells

as well as the survival rate of resting T cells were assessed using flow cytometry. Enzyme-linked immunosorbent assay (ELISA) was applied to determine the secretion levels of IL-2 and IL-17 in the culture supernatant of erianin-treated activated T cells. Western blotting was employed to examine the impact of erianin on the protein expression of cell division cycle protein 2 (CDC2)

phosphorylated CDC2 (p-CDC2)

and Cyclin B1. The differences were observed between the erianin-treated group and the positive control group (activation with CD3/CD28 antibodies).

Results

CFSE proliferation assay demonstrated that the proliferative rate of activated T cells was in a concentration-dependent decline after 0.05~0.20 μmol/L erianin treatment (

0.0001)

with a half-max

imal inhibitory concentration (IC

) of 0.09±0.10 μmol/L. No significant differences were observed in apoptotic rates or survival rates among the erianin-treated groups (activated and resting T cells) and the positive control cells. Analysis of T cell activation markers revealed that erianin had no impact on CD25 expression or IL-2 secretion. However

ELISA results indicated erianin exerted a significant suppression on the secretion of pro-inflammatory cytokine IL-17 (

0.0001). Cell cycle analysis showed that erianin arrested activated T cells at the G2/M phase (

0.05). Further mechanistic investigations demonstrated that while erianin did not alter CDC2 phosphorylation or total CDC2 expression

but it markedly down-regulated CyclinB1 expression (

0.01).

Conclusion

Erianin exhibits potent immunomodulatory activity by suppressing activated T cell proliferation through down-regulating Cyclin B1.

关键词

Keywords

references

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