Effect of thalamic nucleus reuniens on spindle waves in the medial prefrontal cortex during non-rapid eye movement sleep

ZHANG Lei; LU Minmin; ZHANG Xiaolong; HE Chao; XIAO Qin

doi:10.16016/j.2097-0927.202412132

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Effect of thalamic nucleus reuniens on spindle waves in the medial prefrontal cortex during non-rapid eye movement sleep

Monographic Reports | 更新时间：2025-02-27

- Effect of thalamic nucleus reuniens on spindle waves in the medial prefrontal cortex during non-rapid eye movement sleep
- Journal of Army Medical University Vol. 47, Issue 4, Pages: 298-306(2025)
- 作者机构：
  
  陆军军医大学（第三军医大学）基础医学院生理学教研室，重庆
- 作者简介：
- 基金信息：
  
  Chongqing Science Funds for Distinguished Young Scholars(CSTB2022NSCQ-JQX0019)
- DOI：10.16016/j.2097-0927.202412132
  CLC：
- Received：26 December 2024，
  
  Revised：08 January 2025，
  
  Published：28 February 2025
- 稿件说明：
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张磊,陆敏敏,张小龙,等.丘脑连接核对NREM睡眠期内侧前额叶皮层纺锤波的影响[J].陆军军医大学学报,2025,47(04):298-306.

ZHANG Lei,LU Minmin,ZHANG Xiaolong,et al.Effect of thalamic nucleus reuniens on spindle waves in the medial prefrontal cortex during non-rapid eye movement sleep[J].J Army Med Univ,2025,47(04):298-306.
张磊,陆敏敏,张小龙,等.丘脑连接核对NREM睡眠期内侧前额叶皮层纺锤波的影响[J].陆军军医大学学报,2025,47(04):298-306. DOI：10.16016/j.2097-0927.202412132.

ZHANG Lei,LU Minmin,ZHANG Xiaolong,et al.Effect of thalamic nucleus reuniens on spindle waves in the medial prefrontal cortex during non-rapid eye movement sleep[J].J Army Med Univ,2025,47(04):298-306. DOI：10.16016/j.2097-0927.202412132.

摘要

目的

探索丘脑连接核（thalamic nucleus reuniens，RE）在非快速眼动（non-rapid eye movement，NREM）睡眠期间对内侧前额叶皮层（medial prefrontal cortex，mPFC）纺锤波以及神经元活动的影响。

方法

将13只野生型C57BL/6J雄性小鼠（8~12周龄，体质量22~28 g）随机分为实验组（

=7）和对照组（

=6）。采用光遗传抑制技术结合多通道记录技术，在NREM睡眠期间用特异性黄光抑制RE投射到mPFC的神经元末梢20 s，两次光刺激间隔大于5 min。根据神经元的波宽参数和放电频率，鉴定神经元类型和分布情况，并分析抑制后mPFC中纺锤波活动的变化以及中间神经元和锥体神经元的放电变化。

结果

形态学数据验证了RE发出的神经纤维密集的投射到mPFC，且主要分布在深层。与光刺激前相比，实验组小鼠在光刺激期间mPFC的纺锤波活动受到明显影响，主要表现在纺锤波的发生频率降低、中心频率降低以及峰-峰值降低（

0.05），对纺锤波时长、循环个数、对称性及能量值均无影响，同时，在对照组小鼠中的纺锤波指标没有明显变化。小鼠mPFC的中间神经元占比为21.9％，锥体神经元占比为78.1％。光刺激期间实验组中间神经元的放电频率明显下降（

0.05），锥体神经元的放电频率没有明显变化，而对照组小鼠的中间神经元及锥体神经元的放电活动均不受影响。

结论

在NREM睡眠期间，RE输入可能通过激活中间神经元，从而参与mPFC的纺锤波网络振荡的发生。

Abstract

Objective

To determine the effect of thalamic nucleus reuniens (RE) on sleep spindles and neuronal activity in the medial prefrontal cortex (mPFC) during non-rapid eye movement (NREM) sleep.

Methods

Thirteen wild-type C57BL/6 male mice (8~12 weeks old

weighing 22~28 g) were randomly divided into an experimental group (

=7) and a control group (

=6). Optogenetic inhibition combined with multi-channel recording technology was used to specifically inhibit RE projections to mPFC during NREM sleep with yellow light for 20 s

in a 5-min interval between 2 times of light stimuli. The types and distribution of neurons were identified based on the waveform width and firing frequency of neurons. The changes in spindle a

ctivity in the mPFC following inhibition

as well as in firing of interneurons and pyramidal neurons were analyzed.

Results

Morphological data confirmed that neural fibers originating from the RE densely projected to the mPFC

primarily distributed in the deep layers. In the experimental group

light stimulation significantly affected the spindle activity in the mPFC when compared with the period before light stimulation

mainly manifested as a decrease in spindle rate

central frequency

and peak-to-peak amplitude (

0.05). However

no impacts on spindle duration

number of cycles

symmetry

and power were observed. Meanwhile

there were no significant changes in the spindle activity of the control group. The proportion of interneurons in the mPFC area was 21.9%

while pyramidal neurons accounted for 78.1%. The firing rates of interneurons in the experimental group decreased significantly during light stimulation (

0.05)

whereas there was no significant change in the firing rates of pyramidal neurons. In addition

the firing activities of both interneurons and pyramidal neurons in the control group remained unaffected.

Conclusion

During NREM sleep

RE inputs may participate in the generation of sleep spindles network oscillations in the mPFC by activating interneurons.

关键词

Keywords

references

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