Effect of a compound nutrition preparation on wound healing after acute trauma in rats

XIN Wenbin; BAI Qian; NI Xiangmin; HUANG Rongjiang; LIANG Xinyu; MI Mantian; WANG Jian

doi:10.16016/j.2097-0927.202412081

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Effect of a compound nutrition preparation on wound healing after acute trauma in rats

Military Medicine | 更新时间：2025-03-28

- Effect of a compound nutrition preparation on wound healing after acute trauma in rats
- Journal of Army Medical University Vol. 47, Issue 6, Pages: 506-518(2025)
- 作者机构：
  
  1.陆军军医大学（第三军医大学）第二附属医院营养科，重庆
  2.陆军军医大学（第三军医大学）军事预防医学系营养与食品安全研究中心，重庆市医学营养研究中心，营养与健康重庆市重点实验室，重庆
- 作者简介：
  
  MI Mantian， E-mail： mantian_mi@tmmu.edu.cn
  WANG Jian， E-mail： wangjian_xq@tmmu.edu.cn
- 基金信息：
  
  the Major Project of the Fourteeth Five-Year Plan(2022)
- DOI：10.16016/j.2097-0927.202412081
  CLC：
- Received：16 December 2024，
  
  Revised：17 February 2025，
  
  Published：30 March 2025
- 稿件说明：
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辛文彬，白倩，倪向敏，等.一种复方营养制剂对大鼠急性创伤后伤口愈合的影响研究［J］.陆军军医大学学报，2025，47（06）：506-518.DOI：10.16016/j.2097-0927.202412081.

XIN W B，BAI Q，NI X M，et al.Effect of a compound nutrition preparation on wound healing after acute trauma in rats［J］.J Army Med Univ，2025，47（06）：506-518.DOI：10.16016/j.2097-0927.202412081.
辛文彬，白倩，倪向敏，等.一种复方营养制剂对大鼠急性创伤后伤口愈合的影响研究［J］.陆军军医大学学报，2025，47（06）：506-518.DOI：10.16016/j.2097-0927.202412081. DOI：

XIN W B，BAI Q，NI X M，et al.Effect of a compound nutrition preparation on wound healing after acute trauma in rats［J］.J Army Med Univ，2025，47（06）：506-518.DOI：10.16016/j.2097-0927.202412081. DOI：

摘要

目的

一种复方营养制剂对急性创伤后伤口愈合的作用效果及其原理的初步探讨。

方法

60只雄性SD大鼠通过外科手术方法成功构建皮肤创面模型后，采用随机数字表法将大鼠分为对照组（CON组）、乳清蛋白组（whey protein，WP组）、低剂量复方营养制剂组（low-dose nutritional formula，LDF组）和高剂量复方营养制剂组（high-dose nutritional formula，HDF组），每组15只。各组大鼠于术后次日起给予对应营养干预2周，干预期间观察记录各组大鼠的伤口情况并测量伤口面积。分别于术后第3、7、14天取材，采用试剂盒检测血清白蛋白（albumin， ALB）、前白蛋白（prealbumin， PA）、总蛋白（total protein， TP）、炎症因子（TNF-α、IL-1β、IL-6、IL-10）、免疫球蛋白（IgA、IgG、IgM）及羟脯氨酸（hydroxyproline， Hyp）水平；HE染色观察伤口创面组织病理形态学变化；Masson染色观察伤口创面组织胶原纤维沉积情况；免疫组化染色评估伤口创面组织血管生成情况。

结果

与CON组比较，WP组、LDF组、HDF组的创面愈合速度、胶原合成及血管生成速度明显加快（

0.05），其中HDF组效果最明显。术后第3天，与CON组相比，WP组、LDF组和HDF组血清ALB、PA、TP水平显著升高（

0.05），TNF-α、IL-1β水平显著降低（

0.05），而IL-10水平显著升高（WP组除外），LDF组、HDF组血清IgG、IgM水平显著升高（

0.05），HDF组血清IgA水平显著升高（

0.05）；术后第7天，与CON组相比，HDF组ALB、PA和TP水平显著升高（

0.05），TNF-α、IL-1β、IL-6水平显著降低（

0.05），而IL-10水平显著升高（

0.05），LDF组、HDF组血清IgG、IgM水平显著升高（

0.05），HDF组血清IgA水平显著升高（

0.05）；术后第14天，与CON组相比，HDF组ALB、TP水平显著升高（

0.05），IL-1β水平显著降低（

0.05），IgG、IgM水平显著升高（

0.05）。

结论

本研究中的复方营养制剂能够促进急性伤口愈合，其可能原因与制剂能改善大鼠营养状况，促进胶原蛋白合成，减轻机体炎症反应并增强免疫功能有关。

Abstract

Objective‍　‍To explore the efficacy of a compound nutrition preparation on wound healing after acute trauma and investigate the underlying mechanism primarily. Methods‍　‍After skin wound model was successfully constructed on totally 60 male SD rats surgically

they were randomly divided into control group (CON group)

whey protein group (WP group) and low- and high-dose nutritional formula groups (LDF and HDF groups)

with 15 animals in per group. From the next day after surgery

the rats in each group were given co

rresponding nutritional interventions for 2 weeks. During the intervention

the wound conditions were observed and recorded

and the wound area was measured. The samples were collected on the 3rd

7th and 14th days after surgery

respectively. Serum albumin (ALB)

prealbumin (PA)

total protein (TP)

inflammatory factors (TNF-α

IL-1β

IL-6 and IL-10)

immunoglobulins (IgA

IgG and IgM) and hydroxyproline (Hyp) were detected by the corresponding reagent kits. The histopathologic changes of wound were observed with HE staining. Masson staining was used to observe the collagen fiber deposition in wound tissue. The angiogenesis of wound tissue was evaluated by immunohistochemical staining. Results‍　‍Compared with the CON group

the wound healing speed

collagen synthesis and angiogenesis speed were significantly accelerated in the WP group

LDF group and HDF group (

0.05)

and the effects were the most obvious in the HDF group. On the 3rd day after surgery

in the WP

LDF and HDF groups

the serum ALB

PA and TP levels were significantly increased (

0.05)

serum TNF-α and IL-1β levels were obviously decreased (

0.05)

and serum IL-10 level was notably increased (except WP group) when compared with the CON group. The serum IgG and IgM levels were significantly increased in the LDF group and HDF group (

0.05) and significant increment of IgA was only observed in the HDF group (

0.05). On the 7th day after modeling

the levels of ALB

PA and TP were significantly increased (

0.05)

the levels of TNF-α

IL-1β and IL-6 were obviously decreased (

0.05)

and the levels of IL-10 were remarkably increased (

0.05) in the HDF group than the CON group; The LDF group and HDF group had significantly elevated serum IgG and IgM levels (

0.05)

but only the former group had statistically increased serum IgA level (

0.05). On t

he 14th day after modeling

the HDF group had significantly increased levels of ALB and TP (

0.05)

decreased levels of IL-1β (

0.05)

and raised levels of IgG and IgM when compared with the CON group (

0.05). Conclusion‍　‍Our compound nutrition preparation promotes wound healing in rat model of acute trauma

which might be related to its improving the nutritional status

promoting collagen synthesis

and thus alleviating inflammatory response and enhancing immune function.

关键词

Keywords

references

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