Effect of H-NS protein on the virulence of <italic style="font-style: italic">Burkholderia thailandensis</italic>

Leilei WEI; Fei MO; Chunyan WANG; Weiping LU

doi:10.16016/j.2097-0927.202406080

您当前的位置：

首页 >

文章列表页 >

Effect of H-NS protein on the virulence of Burkholderia thailandensis

Basic Medicine | 更新时间：2026-01-09

- Effect of H-NS protein on the virulence of Burkholderia thailandensis
- Journal of Army Medical University Vol. 46, Issue 23, Pages: 2581-2587(2024)
- 作者机构：
  
  400042 重庆，陆军特色医学中心检验科
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(82072348)
- DOI：10.16016/j.2097-0927.202406080
  CLC： R378.99;R446.5;R915
- Received：25 June 2024，
  
  Revised：2024-10-25，
  
  Published：15 December 2024
- 稿件说明：
移动端阅览
Leilei WEI, Fei MO, Chunyan WANG, et al. Effect of H-NS protein on the virulence of Burkholderia thailandensis[J]. Journal of Army Medical University, 2024, 46(23): 2581-2587.
DOI：

Leilei WEI, Fei MO, Chunyan WANG, et al. Effect of H-NS protein on the virulence of Burkholderia thailandensis[J]. Journal of Army Medical University, 2024, 46(23): 2581-2587. DOI： 10.16016/j.2097-0927.202406080.

摘要

目的

构建泰国伯克霍尔德菌BPM的

hns

基因过表达菌株，探究

hns

基因过表达对细菌生长、运动、生物被膜形成能力和抗菌药物敏感性的影响。

方法

将

hns

基因03253和05307分别克隆至表达载体pTAC-Cm上，获得重组载体pTAC- 03253和pTAC- 05307；将两种质粒分别电转至BPM感受态菌株中获得重组菌株，对其生长、运动、生物被膜和药物敏感性的表型变化进行检测。

结果

成功获得

hns

基因过表达菌株；和野生株BPM相比，

hns

基因过表达菌株的生长和运动能力受到明显抑制，生物被膜形成能力显著增强，对左氧氟沙星和米诺环素的敏感性下降。

结论

H-NS蛋白在泰国伯克霍尔德菌BPM毒力和耐药性的调控中发挥重要作用，可成为泰国伯克霍尔德菌感染诊治的新靶点。

Abstract

Objective

To construct

hns

gene overexpression strains of

Burkholderia thailandensis

BPM

and explore the effects of

hns

overexpression on bacterial growth

motility

biofilm formation ability and susceptibility to antimicrobial drugs.

Methods

The

hns

genes 03253 and 05307 were respectively cloned into the expression vector pTAC-Cm to yield recombinant vectors pTAC-03253 and pTAC-05307. Then the 2 types of plasmids were electroporated into the BPM competent strain to obtain recombinant strains

and their phenotypic changes in growth

motility

biofilm

and drug sensitivity were detected.

Results

The strains of

hns

overexpression were successful constructed

and they presented significantly inhibited growth and motility

markedly enhanced ability to

form biofilms

decreased sensitivity to levofloxacin and minocycline when compared with the wild-type BPM strain.

Conclusion

H-NS protein plays an important role in the regulation of virulence and drug resistance in

Burkholderia thailandensis

BPM

and can become a new target for the treatment of infections caused by

Burkholderia thailandensis

关键词

Keywords

references

KASETTHAT T, SERMSWAN R W, REN Y, et al . Decrease of spleen invariant natural killer T and natural killer cell numbers in Burkholderia thailandensis -infected C57BL/6 mice[J ] . Asian Pac J Allergy Immunol, 2022, 40(2): 155-161. DOI: 10.12932/AP-070519-0556..

南栋琪, 文远, 陈建高, 等. 类鼻疽菌Ⅲ型分泌系统BipD蛋白的重组表达及其免疫学特性研究[J]. 陆军军医大学学报, 2024, 46(15): 1713-1720. DOI: 10.16016/j.2097-0927.202311041.

NAN D Q, WEN Y, CHEN J G, et al . Recombinant expression and immunological characterization of Burkholderia pseudomallei type Ⅲ secretion system protein BipD[J ] . Journal of Army Medical University, 2024, 46(15): 1713-1720. DOI: 10.16016/j.2097-0927.202311041.

李进, 黎敏, 鲁卫平. 泰国伯克霍尔德菌感染RAW264.7细胞模型的建立及感染机制的初步探讨[J]. 陆军军医大学学报, 2023, 45(14): 1520-1525. DOI: 10.16016/j.2097-0927.202212159.

LI J, LI M, LU W P. Establishment of a Burkholderia thailandensis invaded RAW264.7 cell model and underlying mechanism of infection[J]. J Army Med Univ, 2023, 45(14): 1520-1525. DOI: 10.16016/j.2097-0927.202212159.

GEE J E, ELROD M G, GULⅥK C A, et al . Burkholderia thailandensis isolated from infected wound, Arkansas, USA[J ] . Emerg Infect Dis, 2018, 24(11): 2091-2094. DOI: 10.3201/eid2411.180821..

LI J, ZHONG Q, LI J, et al . Genomic features and virulence characteristics of a rare Burkholderia thailandensis strain causing human infection[J ] . J Med Microbiol, 2023, 72(5): 1688-1688. DOI: 10.1099/jmm.0.001688..

郑霄, 陈海, 朱雄, 等. 海南省类鼻疽流行区分离出泰国伯克霍尔德菌[J]. 中华流行病学杂志, 2015, 36(1): 97-98. DOI: 10.3760/cma.j.issn.0254-6450.2015.01.023.

ZHENG X, CHEN H, ZHU X, et al . Discovery of Burkholderia thailandensis isolates from melioidosis endemic areas of Hainan, China[J ] . Chin J Epidemiol, 2015, 36(1): 97-98. DOI: 10.3760/cma.j.issn.0254-6450.2015.01.023..

GROISMAN E A, CHOI J. Advancing evolution: Bacteria break down gene silencer to express horizontally acquired genes[J]. Bioessays, 2023, 45(10): e2300062. DOI: 10.1002/bies.202300062.

ALI S S, XIA B, LIU J, et al . Silencing of foreign DNA in bacteria[J ] . Curr Opin Microbiol, 2012, 15(2): 175-181. DOI: 10.1016/j.mib.2011.12.014.

NAVARRE W W. The impact of gene silencing on horizontal gene transfer and bacterial evolution[J]. Adv Microb Physiol, 2016, 69: 157-186. DOI: 10.1016/bs.ampbs.2016.07.004.

SALOMON D, KLIMKO J A, ORTH K. H-NS regulates the Vibrio parahaemolyticus type Ⅵ secretion system 1[J ] . Microbiology, 2014, 160(Pt 9): 1867-1873. DOI: 10.1099/mic.0.080028-0.

AYALA J C, SILVA A J, BENITEZ J A. H-NS: an overarching regulator of the Vibrio cholerae life cycle[J ] . Res Microbiol, 2017, 168(1): 16-25. DOI: 10.1016/j.resmic.2016.07.007.

LIU X X, LI J, ZHANG Z X, et al . Acetylation of xenogeneic silencer H-NS regulates biofilm development through the nitrogen homeostasis regulator in Shewanella [J ] . Nucleic Acids Res, 2024, 52(6): 2886-2903. DOI: 10.1093/nar/gkad1219..

GOMES S C, FERREIRA M R, TAVARES A F, et al . A histone-like nucleoid structuring protein regulates several virulence traits in Burkholderia multivorans [J ] . Appl Environ Microbiol, 2021, 87(14): e0036921. DOI: 10.1128/AEM.00369-21..

RODGERS D, LE C, PIMENTEL C, et al . Histone-like nucleoid-structuring protein (H-NS) regulatory role in antibiotic resistance in Acinetobacter baumannii [J ] . Sci Rep, 2021, 11(1): 18414. DOI: 10.1038/s41598-021-98101-w..

CASTANG S, MCMANUS H R, TURNER K H, et al . H-NS family members function coordinately in an opportunistic pathogen[J ] . Proc Natl Acad Sci U S A, 2008, 105(48): 18947-18952. DOI: 10.1073/pnas.0808215105.

CHOI J, GROISMAN E A. Salmonella expresses foreign genes during infection by degrading their silencer[J ] . Proc Natl Acad Sci U S A, 2020, 117(14): 8074-8082. DOI: 10.1073/pnas.1912808117.

LIU X X, LIN S T, LIU T L, et al . Xenogeneic silencing relies on temperature-dependent phosphorylation of the host H-NS protein in Shewanella [J ] . Nucleic Acids Res, 2021, 49(6): 3427-3440. DOI: 10.1093/nar/gkab137..

FANG F C, RIMSKY S. New insights into transcriptional regulation by H-NS[J]. Curr Opin Microbiol, 2008, 11(2): 113-120. DOI: 10.1016/j.mib.2008.02.011.

XUE X F, ZHNAG M M, SUN J F, et al . H-NS Represses Biofilm Formation and c-di-GMP Synthesis in Vibrio parahaemolyticus [J ] . Biomed Environ Sci, 2022, 35(9): 821-829. DOI: 10.3967/bes2022.106..

LANDINI P, ZEHNDER A J. The global regulatory hns gene negatively affects adhesion to solid surfaces by anaerobically grown Escherichia coli by modulating expression of flagellar genes and lipopolysaccharide production[J ] . J Bacteriol, 2002, 184(6): 1522-1529. DOI: 10.1128/JB.184.6.1522-1529.2002..

冯伟, 夏培元, 孙艺璇, 等. hns 基因对大肠埃希菌生物膜形成能力的影响[J ] . 第三军医大学学报, 2016, 38(2): 107-111. DOI: 10.16016/j.1000-5404.201506016.

FENG W, XIA P Y, SUN Y X, et al . Effect of hns gene on biofilm formation of Escherichia coli [J ] . J Third Mil Med Univ, 2016, 38(2): 107-111. DOI: 10.16016/j.1000-5404.201506016..

郝雪雁, 刘梦晓, 韩紫依, 等. 大肠杆菌的耐酸机制及其改造研究进展[J]. 微生物学通报, 2023, 50(10): 4667-4680. DOI: 10.13344/j.microbiol.china.230136.

HAO X Y, LIU M X, HAN Z Y, et al . Advances in acid-resistant mechanisms and modifications of Escherichia coli [J ] . Microbiol China, 2023, 50(10): 4667-4680. DOI: 10.13344/j.microbiol.china.230136..

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Signal molecule autoinducer 2 promotes biofilm formation and virulence production in Pseudomonas aeruginosa by quorum sensing system

Effect of polypeptide bCAT combined with caspofungin on hypha and mature biofilm in Candida albicans

Screening of antisense peptide nucleic acid sequence targeting lasR gene of Pseudomonas aeruginosa and its inhibition on biofilm formation

Establishment of a Burkholderia thailandensis invaded RAW264.7 cell model and underlying mechanism of infection

Berberine hydrochloride inhibits formation of Staphylococcus epidermidis biofilm: relative gene expressions

Related Author

Xingyuan LI

Qing AI

Liping TAN

Jialin YU

Hongdong LI

Jinglun LIU

Xian LIU

Shan XU

Related Institution

Department of Emergency, National Clinical Research Center for Child Health and Disorders, Key Laboratory of Child Development and Disorders of Ministry of Education, Chongqing Key Laboratory of Pediatrics, Children's Hospital of Chongqing Medical University

Department of Neonates, National Clinical Research Center for Child Health and Disorders, Key Laboratory of Child Development and Disorders of Ministry of Education, Chongqing Key Laboratory of Pediatrics, Children's Hospital of Chongqing Medical University

Department of Pharmacy, People's Hospital of Jiangbei District

Department of Emergency Medicine, the First Affiliated Hospital of Chongqing Medical University

Department of Clinical Laboratory, Institute of Surgery Research, Daping Hospital, Third Military Medical University

⁰

Effect of H-NS protein on the virulence of Burkholderia thailandensis

DOI：10.16016/j.2097-0927.202406080

摘要

Abstract

关键词

Keywords

references