Efficacy of fasciotomy combined with hypertonic saline flushing for crush syndrome in rats
- Journal of Army Medical University Vol. 46, Issue 24, Pages: 2772-2780(2024)
- 作者机构：
1.453000 河南 新乡，新乡医学院第三附属医院骨科
2.400042 重庆，陆军特色医学中心战伤休克与输血研究室，创伤、与化学中毒全国重点实验室
- 作者简介：
- 基金信息：
DOI：10.16016/j.2097-0927.202405077
CLC： R615;R642;R686.3Received：29 May 2024，
Revised：2024-07-28，
Published：30 December 2024
- 稿件说明：
移动端阅览
Yaqian WEN, Xiaoge WANG, Jie ZHANG, et al. Efficacy of fasciotomy combined with hypertonic saline flushing for crush syndrome in rats[J]. Journal of Army Medical University, 2024, 46(24): 2772-2780.
Yaqian WEN, Xiaoge WANG, Jie ZHANG, et al. Efficacy of fasciotomy combined with hypertonic saline flushing for crush syndrome in rats[J]. Journal of Army Medical University, 2024, 46(24): 2772-2780. DOI： 10.16016/j.2097-0927.202405077.
摘要
目的
2
探索筋膜切开术联合高渗NaCl溶液切口冲洗对挤压综合征的疗效。
方法
2
将6周龄雄性SD[体质量(250±20) g]大鼠臀部及双后肢以7.5 kg重物压迫4 h制作挤压综合征大鼠模型
以出现血尿或无尿作为建模成功标准。将24只SD大鼠[体质量(250±20) g]按随机数字表法分为2组: 正常对照组(NC组)10只、挤压综合征组(CS组)14只。在挤压综合征建模完成后的6 h分别收集受挤压组织渗出的组织液
检测各组渗出液中钾离子、钙离子、肌红蛋白和乳酸的含量。另将63只6周龄雄性SD大鼠[体质量(250±20) g]按随机数字表法分为7组
每组9只: 空白对照组(NC组)、挤压综合征组(CSS组)、挤压综合征筋膜切开术组(CSO组); 挤压综合征筋膜切开术联合不同浓度NaCl溶液冲洗组(CSO+0.9%、3.0%、5.0%、7.0% NaCl组)。在挤压完成后的6 h
检测大鼠的肾脏血流情况; 检测各组大鼠血浆中钾离子、钙离子、肌红蛋白、乳酸、AST、ALT、Cr、Urea含量
采集各组大鼠受挤压组织和肾脏组织行病理学检测。
结果
2
组织液检测结果表明
与NC组比较
CS组大鼠组织液中钾离子、肌红蛋白和乳酸含量明显升高
钙离子含量明显降低(
P
<
0.001)。血浆检测结果表明
单纯进行筋膜切开术无明显的治疗作用
筋膜切开术后联合NaCl溶液冲洗可以使挤压综合征大鼠血浆中钾离子
肌红蛋白
乳酸
AST、ALT
Urea、Cr度显著降低
钙离子升高(
P
<
0.05)。在使用激光血流散斑检测中
单纯使用筋膜切开术不能提高肾组织血流
筋膜切开术后联合NaCl溶液冲洗可以使挤压综合征大鼠的肾组织血流量显著提高(
P
<
0.05)。单纯使用筋膜切开术不能减轻肾脏的病理损害
筋膜切开术后联合NaCl溶液冲洗可以减轻挤压综合征对肾脏的病理性损伤(
P
<
0.05)。
结论
2
在大鼠实验中
筋膜切开术联合高浓度(3%)氯化钠溶液切口冲洗后能够显著减轻挤压综合征对机体的损伤。
Abstract
Objective
2
To determine the therapeutic effect of fasciotomy+hypertonic saline flushing for crush syndrome (CS) in rats.
Methods
2
SD rats (weighing 250±20 g) were randomly divided into normal control group (NC group
n
=10) and CS group (
n
=14).Rat CS model was established by compressing the buttocks and both hindlimbs with a weight of 7.5 kg for 4 h
and the presence of hematuria or anuria was defined as success of modeling.In 6 h after modeling
the fluid exuded from the compressed tissues was collected
and the contents of potassium ions (K
+
)
calcium ions (Ca
2+
)
myoglobin (Mb)
and lactic acid (Lac) in the exudate of each group were detected.Another 63 male SD rats (weighing 250±20 g) were randomly and equally divided into blank cont
rol group (NC group)
CS group
CS+fasciotomy group (CSO group)
and CS+fasciotomy+difference doses of NaCl solution irrigation groups (0.9%
3.0%
5.0% and 7.0% NaCl
respectively).At 6 h after modeling
the renal blood flow was measured
the contents of K
+
Ca
2+
Mb
Lac
AST
ALT
Cr
and urea in the plasma of each group were detected
and the compressed tissues and kidney tissues were observed for pathological changes.
Results
2
Detection of exudates showed the contents of K
+
Mb
and Lac were significantly higher
while that of Ca
2+
content was obviously decreased in the CS group than the NC group (
P
<
0.01).Plasma detection indicated that simple fasciotomy had no therapeutic effect
while it combined with NaCl solution flushing decreased the contents of K
+
Mb
Lac
AST
ALT
urea and Cr
and increased the Ca
2+
content in the blood of the CS group (
P
<
0.05).Laser speckle contrast imaging revealed that simple fasciotomy could not increase renal blood flow
while the combination of fasciotomy and NaCl solution flushing notably increased the renal blood flow in the CS rats (
P
<
0.05).In addition
the combination treatment reduced the pathological damage in the kidneys induced by CS
but fasciotomy alone had no such effect (
P
<
0.5).
Conclusion
2
Fasciotomy combined with hypertonic NaCl solution (3%) flushing can significantly reduce the damage caused by CS in rats.
关键词
Keywords
references
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