Diversity and composition changes of intestinal Fungi in a mouse model of total parenteral nutrition

Huichao XIE; Yihui CHEN; Xingyu LIU; Ben HAN; Lihua SUN; Weidong XIAO

doi:10.16016/j.2097-0927.202404125

您当前的位置：

首页 >

文章列表页 >

Diversity and composition changes of intestinal Fungi in a mouse model of total parenteral nutrition

Basic Medicine | 更新时间：2026-01-09

- Diversity and composition changes of intestinal Fungi in a mouse model of total parenteral nutrition
- Journal of Army Medical University Vol. 46, Issue 21, Pages: 2407-2414(2024)
- 作者机构：
  
  1.400037 重庆，陆军军医大学(第三军医大学)第二附属医院普通外科
  2.400037 重庆，陆军军医大学(第三军医大学)第二附属医院营养科
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(82270585);the High-Tech Incubation Project of Military Medicine(CX2019JS212)
- DOI：10.16016/j.2097-0927.202404125
  CLC： Q949.32;R-332;R459.3
- Received：26 April 2024，
  
  Revised：2024-06-27，
  
  Published：15 November 2024
- 稿件说明：
移动端阅览
Huichao XIE, Yihui CHEN, Xingyu LIU, et al. Diversity and composition changes of intestinal Fungi in a mouse model of total parenteral nutrition[J]. Journal of Army Medical University, 2024, 46(21): 2407-2414.
DOI：

Huichao XIE, Yihui CHEN, Xingyu LIU, et al. Diversity and composition changes of intestinal Fungi in a mouse model of total parenteral nutrition[J]. Journal of Army Medical University, 2024, 46(21): 2407-2414. DOI： 10.16016/j.2097-0927.202404125.

摘要

目的

研究小鼠在全肠外营养(total parenteral nutrition，TPN)模型下与正常小鼠相比，肠道中真菌群落的多样性以及组成结构的改变。

方法

建立TPN小鼠模型后，采集TPN模型小鼠(

=5)和正常对照小鼠(

=5)的新鲜粪便样本，利用ITS DNA测序技术对肠道真菌进行测定，然后通过生物信息学方法对两组小鼠的真菌菌群多样性、群落结构及功能特征的差异进行分析。

结果

TPN模型小鼠和正常对照小鼠的肠道真菌Alpha多样性具有明显的差异(

＜0.05)；Beta多样性存在统计学差异(

＜0.01)；在TPN模型小鼠中，

Candida

(假丝酵母属)的相对丰度明显减少(

＜0.01)，

Penicillium

(青霉菌属)的相对丰度明显减少(

＜0.01)，

Aspergillus

(曲霉菌属)的相对丰度明显减少(

＜0.01)，

Talaromyces

(

篮状菌属)的相对丰度明显减少(

＜0.01)。LEfSe分析揭示，在正常对照小鼠中，

Candida

(假丝酵母属)，

Penicillium

(青霉菌属)，

Aspergillus

(曲霉菌属)和

Talaromyces

(篮状菌属)有明显的富集。

结论

与正常小鼠相比，TPN模型小鼠的肠道真菌菌群组织结构发生明显改变。肠道真菌菌群失调可能促进TPN相关并发症的发生，调节肠道真菌群落平衡可能成为预防TPN相关并发症的一种新策略。

Abstract

Objective

To analyze the diversity and composition changes of gut fungal communities between mouse model of total parental nutrition (TPN) and normal control mice.

Methods

After mouse model of TPN was constructed

fresh feces were collected from TPN mice (

=5) and normal control mice (

=5). Internal transcribed spacer (ITS) DNA sequencing was applied to determine intestinal fungi

and then bioinformatics analysis was conducted to identify the differences in fungal diversity

structure

and functional properties between the 2 groups of mice.

Results

There were significant differences in Alpha diversity (

0.05) and Beta diversity (

0.01) of intestinal fungi between the 2 groups. In the TPN model group

the relative abundances of

Candida

Penicillium

Aspergillus

and

Talaromyces

were obviously reduced (all

0.01). LEfSe analysis indicated that the above 4 strains were notably enriched in the normal control mice.

Conclusion

TPN mice exhibit characteristic changes in the composition of gut fungal flora compared to normal control mice. Dysfunction of gut fungal community may promote the occurrence of TPN related complications

and regulating the balance of gut fungal community may become a new strategy for preventing TPN related complications.

关键词

Keywords

references

JAIN A K, WEN J X, ARORA S, et al . Validating hyperbilirubinemia and gut mucosal atrophy with a novel ultramobile ambulatory total parenteral nutrition piglet model[J ] . Nutr Res, 2015, 35(2): 169-174. DOI: 10.1016/j.nutres.2014.12.004.

PYBUS R, PUNTIS J W. Parenteral nutrition[J]. Arch Dis Child, 2021, 106(9): 921-922. DOI: 10.1136/archdischild-2020-319647.

DIAMANTI A, PUNTIS J, KOLACEK S, et al . Chapter 5.2.3. parenteral nutrition and home parenteral nutrition changed the face of paediatric gastroenterology[J ] . J Pediatr Gastroenterol Nutr, 2018, 66(Suppl 1): S82-S87. DOI: 10.1097/MPG.0000000000001915.

JOHNSON M J, LAPILLONNE A, BRONSKY J, et al . Research priorities in pediatric parenteral nutrition: a consensus and perspective from ESPGHAN/ESPEN/ESPR/CSPEN[J ] . Pediatr Res, 2022, 92(1): 61-70. DOI: 10.1038/s41390-021-01670-9.

WANG P, SUN H F, MAITIABULA G, et al . Total parenteral nutrition impairs glucose metabolism by modifying the gut microbiome[J ] . Nat Metab, 2023, 5(2): 331-348. DOI: 10.1038/s42255-023-00744-8.

DENTON C, PRICE A, FRIEND J, et al . Role of the gut liver axis in driving parenteral nutrition-associated injury[J ] . Children, 2018, 5(10): 136. DOI: 10.3390/children5100136.

MILANI C, DURANTI S, BOTTACINI F, et al . The first microbial colonizers of the human gut: composition, activities, and health implications of the infant gut microbiota[J ] . Microbiol Mol Biol Rev, 2017, 81(4): e00036-17. DOI: 10.1128/MMBR.00036-17.

ZHANG T, LIU Y, YAN J K, et al . Early downregulation of P-glycoprotein facilitates bacterial attachment to intestinal epithelial cells and thereby triggers barrier dysfunction in a rodent model of total parenteral nutrition[J ] . FASEB J, 2020, 34(3): 4670-4683. DOI: 10.1096/fj.201902513R.

DAHLGREN A F, PAN A, LAM V, et al . Longitudinal changes in the gut microbiome of infants on total parenteral nutrition[J ] . Pediatr Res, 2019, 86(1): 107-114. DOI: 10.1038/s41390-019-0391-y.

CAHOVA M, BRATOVA M, WOHL P. Parenteral nutrition-associated liver disease: the role of the gut microbiota[J]. Nutrients, 2017, 9(9): 987. DOI: 10.3390/nu9090987.

CERDÓT, GARCÍA-SANTOS J A, RODRÍGUEZ-PÖHNLEIN A, et al . Impact of total parenteral nutrition on gut microbiota in pediatric population suffering intestinal disorders[J ] . Nutrients, 2022, 14(21): 4691. DOI: 10.3390/nu14214691.

LI X V, LEONARDI I, ILIEV I D. Gut mycobiota in immunity and inflammatory disease[J]. Immunity, 2019, 50(6): 1365-1379. DOI: 10.1016/j.immuni.2019.05.023.

UNDERHILL D M, ILIEV I D. The mycobiota: interactions between commensal fungi and the host immune system[J]. Nat Rev Immunol, 2014, 14(6): 405-416. DOI: 10.1038/nri3684.

ILIEV I D, LEONARDI I. Fungal dysbiosis: immunity and interactions at mucosal barriers[J]. Nat Rev Immunol, 2017, 17(10): 635-646. DOI: 10.1038/nri.2017.55.

BERING J, DIBAISE J K. Home parenteral and enteral nutrition[J]. Nutrients, 2022, 14(13): 2558. DOI: 10.3390/nu14132558.

HOWARD L, MALONE M. Clinical outcome of geriatric patients in the United States receiving home parenteral and enteral nutrition[J]. Am J Clin Nutr, 1997, 66(6): 1364-1370. DOI: 10.1093/ajcn/66.6.1364.

YAN X, ZHANG S Y, JIA J M, et al . Total parenteral nutrition treatment improves the nutrition status of gynecological cancer patients by improving serum albumin level[J ] . Front Med, 2021, 8: 759387. DOI: 10.3389/fmed.2021.759387.

EKELUND M, KRISTENSSON E, EKELUND M, et al . Total parenteral nutrition causes circumferential intestinal atrophy, remodeling of the intestinal wall, and redistribution of eosinophils in the rat gastrointestinal tract[J ] . Dig Dis Sci, 2007, 52(8): 1833-1839. DOI: 10.1007/s10620-006-9678-z.

NOGUCHI M, MORIYA T, MURAKOSHI S, et al . Lipid compositions of total parenteral nutrition affect gut peyer's patches and morphology in mice[J ] . J Surg Res, 2022, 280: 355-362. DOI: 10.1016/j.jss.2022.07.004.

KUMPF V J. Parenteral nutrition-associated liver disease in adult and pediatric patients[J]. Nut Clin Prac, 2006, 21(3): 279-290. DOI: 10.1177/0115426506021003279.

TOMAR B S. Hepatobiliary abnormalities and parenteral nutrition[J]. Indian J Pediatr, 2000, 67(9): 695-701. DOI: 10.1007/BF02762189.

WANG H, WU H, LI K D, et al . Intestinal fungi and systemic autoimmune diseases[J ] . Autoimmun Rev, 2023, 22(2): 103234. DOI: 10.1016/j.autrev.2022.103234.

CHEHOUD C, ALBENBERG L G, JUDGE C, et al . Fungal signature in the gut microbiota of pediatric patients with inf lammatory bowel disease[J ] . Inflamm Bowel Dis, 2015, 21(8): 1948-1956. DOI: 10.1097/MIB.0000000000000454.

WANG T T, FAN C G, YAO A R, et al . The adaptor protein CARD9 protects against colon cancer by restricting mycobiota-mediated expansion of myeloid-derived suppressor cells[J ] . Immunity, 2018, 49(3): 504-514. e4. DOI: 10.1016/j.immuni.2018.08.018.

KROHN S, ZELLER K, BÖHM S, et al . Molecular quantification and differentiation of Candida species in biological specimens of patients with liver cirrhosis[J ] . PLoS One, 2018, 13(6): e0197319. DOI: 10.1371/journal.pone.0197319.

TANG C, KAMIYA T, LIU Y, et al . Inhibition of dectin-1 signaling ameliorates colitis by inducing Lactobacillus-mediated regulatory T cell expansion in the intestine[J ] . Cell Host Microbe, 2015, 18(2): 183-197. DOI: 10.1016/j.chom.2015.07.003.

QIU X Y, ZHANG F, YANG X, et al . Changes in the composition of intestinal fungi and their role in mice with dextran sulfate sodium-induced colitis[J ] . Sci Rep, 2015, 5: 10416. DOI: 10.1038/srep10416.

SOKOL H, LEDUCQ V, ASCHARD H, et al . Fungal microbiota dysbiosis in IBD[J ] . Gut, 2017, 66(6): 1039-1048. DOI: 10.1136/gutjnl-2015-310746.

QAMAR A, ABOUDOLA S, WARNY M, et al . Saccharomyces boulardii stimulates intestinal immunoglobulin A immune response to Clostridium difficile toxin A in mice[J ] . Infect Immun, 2001, 69(4): 2762-2765. DOI: 10.1128/IAI.69.4.2762-2765.2001.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

多囊肾病患者肠道真菌特征性改变及其与临床指标的相关性研究

Diversity and composition changes of intestinal fungi in patients with chronic kidney disease

Changes in intestinal fungal diversity and compositions in patients with cholestatic liver disease

Related Author

YANG Shiming

CHENG Jinbo

JIAN Jincheng

JIANG Hongfei

HOU Zhanjie

GE Yusong

TANG Bo

LEI Yuanyuan

Related Institution

Department of General Surgery, Second Affiliated Hospital， Army Medical University （Third Military Medical University）

Department of Nutrition, Second Affiliated Hospital， Army Medical University （Third Military Medical University）

Department of Pathogen Biology, College of Basic Medicine and Forensic Medicine, Affiliated Hospital, North Sichuan Medical College, Nanchong

Department of Gastroenterology, Second Affiliated Hospital, Army Medical University (Third Military Medical University)

⁰