Correlation between salivary cystatin D level and salivary gland injury in patients with primary Sjögren syndrome

Jing WANG; Fei LI; Yi HUANG; Lingfei MO; Hanchao LI; Ying PAN; Xiuyuan FENG; Xinyi LIU; Yuanyuan LI

doi:10.16016/j.2097-0927.202403115

您当前的位置：

首页 >

文章列表页 >

Correlation between salivary cystatin D level and salivary gland injury in patients with primary Sjögren syndrome

Clinical Medicine | 更新时间：2026-01-09

- Correlation between salivary cystatin D level and salivary gland injury in patients with primary Sjögren syndrome
- Journal of Army Medical University Vol. 46, Issue 21, Pages: 2451-2456(2024)
- 作者机构：
  
  1.710061 西安，西安交通大学第一附属医院风湿免疫科
  2.400038 重庆，陆军军医大学(第三军医大学)生物医学分析测试中心
- 作者简介：
- 基金信息：
  
  the General Project of Natural Science Foundation of Shaanxi Province(2023-JC-YB-754)
- DOI：10.16016/j.2097-0927.202403115
  CLC： R442.8;R446.19;R781.7
- Received：28 March 2024，
  
  Revised：2024-06-11，
  
  Published：15 November 2024
- 稿件说明：
移动端阅览
Jing WANG, Fei LI, Yi HUANG, et al. Correlation between salivary cystatin D level and salivary gland injury in patients with primary Sjögren syndrome[J]. Journal of Army Medical University, 2024, 46(21): 2451-2456.
DOI：

Jing WANG, Fei LI, Yi HUANG, et al. Correlation between salivary cystatin D level and salivary gland injury in patients with primary Sjögren syndrome[J]. Journal of Army Medical University, 2024, 46(21): 2451-2456. DOI： 10.16016/j.2097-0927.202403115.

摘要

目的

探讨原发性干燥综合征(primary Sjögren syndrome

pSS)患者唾液胱抑素D水平与唾液腺损伤的相关性。

方法

纳入2022年9月1日至2023年6月30日就诊于西安交通大学第一附属医院风湿免疫科pSS组患者51例及年龄、性别匹配的来院健康体检对照组人员51例。检测唾液中胱抑素D水平，采用独立样本

检验评估两组胱抑素D水平的差异，采用Pearson相关性检验评估pSS组胱抑素D与临床指标的关系。

结果

pSS组唾液中胱抑素D水平明显低于对照组(206.55±108.11

374.32±172.24 pg/mL

0.01)。pSS患者胱抑素D水平与其静态唾液流率(

=0.433

=0.002)及动态唾液流率(

=0.363

=0.009)呈正相关。高唾液腺超声检查评分的pSS患者唾液胱抑素D水平明显低于低评分患者(腮腺：160.75±85.56

290.53±95.17 pg/mL

＜0.01；颌下腺：157.76±87.59

276.25±97.06 pg/mL

＜0.01)。pSS患者胱抑素D水平与外周血IL-6水平(

=-0.453

=0.001)及CD4

T细胞计数(

=-0.396

=0.005)呈负相关。

结论

pSS患者唾液胱抑素D水平可作为反映唾液腺损伤的指标。

Abstract

Objective

To investigate the correlation between salivary cystatin D level and salivary gland injury in patients with primary Sjögren syndrome (pSS).

Methods

A total of 51 pSS patients admitted in the Department of Rheumatology and Immunology of the First Affiliated Hospital of Xi'an Jiaotong University from September 1

2022 to June 30

2023

and 51 age- and gender-matched healthy individuals who took physical examination in the hospital during same period were enrolled in the study. The

level of salivary cystatin D was detected

and the difference in the level between the 2 groups was compared using an independent-samples

test. Pearson correlation analysis was applied to analyze the correlation between salivary cystatin D and clinical parameters in the patients.

Results

The pSS patients had significantly lower cystatin D level than the healthy controls (206.55±108.11

374.32±172.24 pg/mL

0.01). The cystatin D level in the pSS patients was positively correlated with both static (

=0.433

=0.002) and dynamic salivary flow rates (

=0.363

=0.009). The patients with higher score of salivary gland ultrasonography (SGUS) had obviously lower cystatin D than those with lower SGUS score (parotid gland: 160.75±85.56

290.53±95.17 pg/mL

0.01; submandibular gland: 157.76±87.59

276.25±97.06 pg/mL

0.01). The cystatin D level was also negatively correlated with peripheral blood IL-6 level (

=-0.453

=0.001) and CD4

T cell count (

=-0.396

=0.005) in the pSS patients.

Conclusion

Salivary cystatin D level can be used as an indicator of salivary gland damage for pSS patients.

关键词

Keywords

references

BEYDON M, MCCOY S, NGUYEN Y, et al . Epidemiology of Sjögren syndrome[J ] . Nat Rev Rheumatol, 2024, 20(3): 158-169. DOI: 10.1038/s41584-023-01057-6.

RAMOS-CASALS M, BRITO-ZERÓN P, BOMBARDIERI S, et al . EULAR recommendations for the management of Sjögren's syndrome with topical and systemic therapies[J ] . Ann Rheum Dis, 2020, 79(1): 3-18. DOI: 10.1136/annrheumdis-2019-216114.

QIN B D, WANG J Q, YANG Z X, et al . Epidemiology of primary Sjögren's syndrome: a systematic review and meta-analysis[J ] . Ann Rheum Dis, 2015, 74(11): 1983-1989. DOI: 10.1136/annrheumdis-2014-205375.

JUNG J Y, KIM J W, KIM H A, et al . Salivary biomarkers in patients with Sjögren's syndrome—a systematic review[J ] . Int J Mol Sci, 2021, 22(23): 12903. DOI: 10.3390/ijms222312903.

SERRAO S, CONTINI C, GUADALUPI G, et al . Salivary cystatin D interactome in patients with systemic mastocytosis: an exploratory study[J ] . Int J Mol Sci, 2023, 24(19): 14613. DOI: 10.3390/ijms241914613.

刘羽璇, 魏华. 与干燥综合征相关的新型抗体研究进展[J]. 中华风湿病学杂志, 2022, 26(7): 493-496. DOI: 10.3760/cma.j.cn141217-20211124-00477.

LIU Y X, WEI H. Research progress of new antibodies related to Sjogren's syndrome[J]. Chin J Rheumatol, 2022, 26(7): 493-496. DOI: 10.3760/cma.j.cn141217-20211124-00477.

王小宇, 吴红, 王勇, 等. 抗水通道蛋白抗体1和5在原发性干燥综合征的诊断价值[J]. 陆军军医大学学报, 2023, 45(8): 835-841. DOI: 10.16016/j.2097-0927.202208201.

WANG X Y, WU H, WANG Y, et al . Diagnostic value of aquaporin 1 and 5 antibodies in primary Sjögren's syndrome[J ] . J Army Med Univ, 2023, 45(8): 835-841. DOI: 10.16016/j.2097-0927.202208201.

NONAKA T, WONG D T W. Saliva diagnostics[J]. Annu Rev Anal Chem, 2022, 15(1): 107-121. DOI: 10.1146/annurev-anchem-061020-123959.

AQRAWI L A, GALTUNG H K, GUERREIRO E M, et al . Proteomic and histopathological characterisation of sicca subjects and primary Sjögren's syndrome patients reveals promising tear, saliva and extracellular vesicle disease biomarkers[J ] . Arthritis Res Ther, 2019, 21(1): 181. DOI: 10.1186/s13075-019-1961-4.

HYNNE H, AQRAWI L A, JENSEN J L, et al . Proteomic profiling of saliva and tears in radiated head and neck cancer patients as compared to primary Sjögren's syndrome patients[J ] . Int J Mol Sci, 2022, 23(7): 3714. DOI: 10.3390/ijms23073714.

FREIJE J P, BALBÍN M, ABRAHAMSON M, et al . Human cystatin D. cDNA cloning, characterization of the Escherichia coli expressed inhibitor, and identification of the native protein in saliva[J ] . J Biol Chem, 1993, 268(21): 15737-15744..

HÜNTEN S, HERMEKING H. p53 directly activates cystatin D/CST5 to mediate mesenchymal-epithelial transition: a possible link to tumor suppression by vitamin D3[J]. Oncotarget, 2015, 6(18): 15842-15856. DOI: 10.18632/oncotarget.4683.

FERRER-MAYORGA G, ALVAREZ-DÍAZ S, VALLE N, et al . Cystatin D locates in the nucleus at sites of active transcription and modulates gene and protein expression[J ] . J Biol Chem, 2015, 290(44): 26533-26548. DOI: 10.1074/jbc.M115.660175.

FUJITA-YOSHIGAKI J, YOKOYAMA M, KATSUMATA-KATO O. Switching of cargo sorting from the constitutive to regulated secretory pathway by the addition of cystatin D sequence in salivary acinar cells[J]. Am J Physiol Gastrointest Liver Physiol, 2020, 319(1): G74-G86. DOI: 10.1152/ajpgi.00103.2020.

DENCKER M, TANHA, KARLSSON M K, et al . Cystatin B, cathepsin L and D related to surrogate markers for cardiovascular disease in children[J ] . PLoS One, 2017, 12(11): e0187494. DOI: 10.1371/journal.pone.0187494.

DAS N C, GUPTA P S S, PANDA S K, et al . Reverse vaccinology assisted design of a novel multi-epitope vaccine to target Wuchereria bancrofti cystatin: an immunoinformatics approach[J ] . Int Immunopharmacol, 2023, 115: 109639. DOI: 10.1016/j.intimp.2022.109639..

HILL L J, PIETRO V D, HAZELDINE J, et al . Cystatin D (CST5): an ultra-early inflammatory biomarker of traumatic brain injury[J ] . Sci Rep, 2017, 7(1): 5002. DOI: 10.1038/s41598-017-04722-5.

何思慧, 张剑勇, 贾二涛. 原发性干燥综合征炎症发病机制[J]. 中国免疫学杂志, 2023, 39(12): 2679-2683, 2689. DOI: 10.3969/j.issn.1000-484X.2023.12.037.

HE S H, ZHANG J Y, JIA E T. Inflammatory pathogenesis of primary Sjögren's syndrome[J]. Chin J Immunol, 2023, 39(12): 2679-2683, 2689. DOI: 10.3969/j.issn.1000-484X.2023.12.037.

赵星星, 刘杨, 郝慧琴. 辅助性T细胞17与调节性T细胞在干燥综合征发病机制中的作用[J]. 中华风湿病学杂志, 2023, 27(7): 491-496. DOI: 10.3760/cma.j.cn141217-20221201-00489.

ZHAO X X, LIU Y, HAO H Q. The role of T helper 17 (Th17) and regulatory T cells (Treg) in the pathogenesis of Sjögren's syndrome[J]. Chin J Rheumatol, 2023, 27(7): 491-496. DOI: 10.3760/cma.j.cn141217-20221201-00489.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

No data

Related Author

No data

Related Institution

No data

⁰