Correlation between cancer related anemia and abundance of SARS-CoV-2 specific memory CD8<sup>+</sup> T cells

Xuezhi YANG; Junlei HOU; Enjian ZHU; Haixia LONG; Haoran ZHA; Bo ZHU

doi:10.16016/j.2097-0927.202402058

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Correlation between cancer related anemia and abundance of SARS-CoV-2 specific memory CD8⁺ T cells

Clinical Medicine | 更新时间：2026-01-09

- Correlation between cancer related anemia and abundance of SARS-CoV-2 specific memory CD8⁺ T cells
- Journal of Army Medical University Vol. 46, Issue 9, Pages: 1047-1056(2024)
- 作者机构：
  
  1.400037 重庆，陆军军医大学(第三军医大学)第二附属医院全军肿瘤研究所
  2.130062 长春，中国人民解放军第九六四医院放射科
  3.100088 北京，中国人民解放军火箭军特色医学中心肿瘤科
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(82272808)
- DOI：10.16016/j.2097-0927.202402058
  CLC： R373.1;R392.12;R556.9
- Received：21 February 2024，
  
  Revised：2024-03-08，
  
  Published：15 May 2024
- 稿件说明：
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Xuezhi YANG, Junlei HOU, Enjian ZHU, et al. Correlation between cancer related anemia and abundance of SARS-CoV-2 specific memory CD8⁺ T cells[J]. Journal of Army Medical University, 2024, 46(9): 1047-1056.
DOI：

Xuezhi YANG, Junlei HOU, Enjian ZHU, et al. Correlation between cancer related anemia and abundance of SARS-CoV-2 specific memory CD8⁺ T cells[J]. Journal of Army Medical University, 2024, 46(9): 1047-1056. DOI： 10.16016/j.2097-0927.202402058.

摘要

目的

探讨肿瘤相关性贫血对外周血中新型冠状病毒特异性记忆CD8

T细胞丰度的影响。

方法

纳入2023年6-7月在火箭军特色医学中心住院治疗的HLA-A*11∶01阳性中晚期实体瘤患者34例。根据血红蛋白浓度将患者分为无贫血组(HGB≥120 g/L，

=14)、轻度贫血组(120 g/L

HGB≥90 g/L，

=14)与中重度贫血组(HGB

90 g/L，

=6)。分离外周血单个核细胞，利用新冠病毒抗原表位刺激并扩增记忆CD8

T细胞，使用ELISPOT筛选阳性表位，构建Tetramer。利用扩增后细胞检测Tetramer

细胞比例、数量及ELISPOT斑点数，用以反映新冠病毒特异性记忆CD8

T细胞丰度。分析患者血红蛋白浓度与Tetramer

细胞比例、数量及ELISPOT斑点数的相关性。分析不同贫血组Tetramer

细胞比例、数量及ELISPOT斑点数的差异。

结果

34例患者中男性25例，女性9例；年龄36~78岁，中位年龄57岁。患者扩增后Tetramer

细胞比例、数量及ELISPOT斑点数与血红蛋白浓度均呈正相关关系(

＜0.05)；与无贫血组相比，中重度贫血组患者Tetramer

细胞比例、数量及ELISPOT斑点数均明显降低(

＜0.05)。

结论

肿瘤患者外周血新冠病毒特异性记忆CD8

T细胞的丰度与血红蛋白浓度正相关，提示肿瘤相关性贫血可能通过降低外周血中记忆CD8

T细胞的丰度导致患者病毒易感性增高。

Abstract

Objective

To explore the effect of cancer related anemia on the abundance of SARS-CoV-2 specific memory CD8

T cells in peripheral blood.

Methods

A total of 34 HLA-A*11∶01 positive patients with middle- and late-stage tumors admitted in the Rocket Force Medical Center of PLA from June to July 2023 were enrolled in this study. According to hemoglobin (HGB) level

they were divided into non anemia group (≥120 g/L

=14)

mild anemia group (90~120 g/L

=14)

and moderate to severe anemia group (

90 g/L

=6). After their peripheral blood mononuclear cells were isolated

memory CD8

T cells were stimulated by the epitopes of SARS-CoV-2 and then expanded. Positive epitopes were screened using enzyme-linked immunosorbent spot (ELISPOT) assay

and then MHC-peptide Tetramers were constructed. The proportion and number of Tetramer

cells and the number of ELISPOT spots were detected in the expanded cells to reflect the abundance of SARS-CoV-2 specific memory CD8

T cells. The relevance of patients' HGB level with the proportion and number of Tetramer

cells and the number of ELISPOT spots was analyzed. The differences of the proportion and number of Tetramer

cells and the number of ELISPOT spots was analyzed in different groups.

Results

Among the 34 patients

there were 25 males and 9 females

at an age range of 36~78 (median 57) years. The proportion and number of Tetramer

cells and the number of ELISPOT spots were positively correlated with HGB level (

0.05). The proportion and number of Tetramer

cells and the number of ELISPOT spots were significantly decreased in patients with moderate to severe anemia than the non anemia patients (

0.05).

Conclusion

The abundance of SARS-CoV-2 specific memory CD8

T cells in peripheral blood is positively correlated with patients' HGB level

indicating that CRA may increase the susceptibility of patients to viruses by reducing the abundance of memory CD8

T cells in peripheral blood.

关键词

Keywords

references

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Related Institution

Institute of Cancer， Xinqiao Hospital， Army Medical University （Third Military Medical University）

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Department of Oncology， PLA Rocket Force Characteristic Medical Center

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⁰

Correlation between cancer related anemia and abundance of SARS-CoV-2 specific memory CD8+ T cells

DOI：10.16016/j.2097-0927.202402058

摘要

Abstract

关键词

Keywords

references

Correlation between cancer related anemia and abundance of SARS-CoV-2 specific memory CD8⁺ T cells