Detection of <italic style="font-style: italic">Brucella abortus</italic> using an electrochemical immunosensor modified with PB-MWCNTs-GNPs

Yu CHI; Yu CAO; Hao CHENG; Jingwen CAO; Jianyue AO; Haibo LI; Lei MA; Ming LIU

doi:10.16016/j.2097-0927.202401080

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Detection of Brucella abortus using an electrochemical immunosensor modified with PB-MWCNTs-GNPs

Basic Medicine | 更新时间：2026-01-09

- Detection of Brucella abortus using an electrochemical immunosensor modified with PB-MWCNTs-GNPs
- Journal of Army Medical University Vol. 46, Issue 17, Pages: 1969-1975(2024)
- 作者机构：
  
  1.154007 黑龙江佳木斯，佳木斯大学临床医学院
  2.400038 重庆，陆军军医大学(第三军医大学)药学与检验医学系
  3.401320 重庆，重庆医科大学附属巴南医院检验科
  4.401331 重庆，重庆市沙坪坝区陈家桥医院检验科
- 作者简介：
- 基金信息：
- DOI：10.16016/j.2097-0927.202401080
  CLC： R318.08;R378.5;R446.61
- Received：20 January 2024，
  
  Revised：2024-04-18，
  
  Published：15 September 2024
- 稿件说明：
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Yu CHI, Yu CAO, Hao CHENG, et al. Detection of Brucella abortus using an electrochemical immunosensor modified with PB-MWCNTs-GNPs[J]. Journal of Army Medical University, 2024, 46(17): 1969-1975.
DOI：

Yu CHI, Yu CAO, Hao CHENG, et al. Detection of Brucella abortus using an electrochemical immunosensor modified with PB-MWCNTs-GNPs[J]. Journal of Army Medical University, 2024, 46(17): 1969-1975. DOI： 10.16016/j.2097-0927.202401080.

摘要

目的

构建一种高性能的电化学免疫传感器用于检测流产布鲁氏菌。

方法

制备普鲁士蓝(prussian blue，PB)-多壁碳纳米管(multi walled carbon nanotubes，MWCNTs)-纳米金粒子(gold nanoparticles，GNPs)纳米复合物，以流产布鲁氏菌作为待测样本，选择合适的抗体构建电化学免疫传感器，通过对传感器构建关键因素的考察确定最优条件，最后对传感器进行性能评价。

结果

明确传感器最优构建条件为：MWCNTs-PB混合比例为1 ∶5，材料干燥温度为37 ℃，缓冲体系最佳pH=7.5，抗体孵育时间为1 h，样本孵育时间为30 min；流产布鲁氏菌在10~1×10

CFU/mL范围内呈良好的线性关系，传感器抗干扰能力、检测重复性与稳定性好，准确度高。

结论

基于PB-MWCNTs-GNPs纳米材料所修饰的用于检测流产布鲁氏菌的电化学免疫传感器构建简便，性能良好，可为布鲁氏病的临床早期诊断提供参考。

Abstract

Objective

To prepare a high performance electrochemical immunosensor for detecting

Brucella abortus

(

B. abortus

Methods

Prussian blue (PB)

multi walled carbon nanotubes (MWCNTs) and gold nanoparticles (GNPs)(PB-MWCNTs-GNPs) nanocomposites were prepared

and appropriate antibody was used to construct the immunosensor for detecting

B. abortus

samples. The optimal conditions were clarified by examining the key factors in sensor construction

and then the performance of the sensor was evaluated.

Results

The optimal construction conditions were determined as follows: the ratio of MWCNTs-PB was 1 ∶5

the drying temperature was 37 ℃

the pH value of buffer system was 7.5

and the incubation time of antibody and sample was 1 h and 30 min

respectively.

B. abortus

exhibited a good linear relationship

when ranging from 10 to 1×10

CFU/mL. The sensor had good anti-interference ability

repeatability

stability and high accuracy.

Conclusion

Our prepared PB-MWCNTs GNPs nanomaterials modified electrochemical immunosensor for detecting

B. abortus

is easy to prepare

has good performance

and can provide reference for the early clinical diagnosis of brucellosis.

关键词

Keywords

references

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Detection of Brucella abortus using an electrochemical immunosensor modified with PB-MWCNTs-GNPs

DOI：10.16016/j.2097-0927.202401080

摘要

Abstract

关键词

Keywords

references