<italic style="font-style: italic">BNC</italic>1 promotes cell migration and invasion of lung squamous cell carcinoma

Longyao ZHANG; Longjin ZENG; Linpeng ZHENG; Fenglin LIN; Dingqin CAI; Ping CAI; Jianguo SUN

doi:10.16016/j.2097-0927.202312131

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BNC1 promotes cell migration and invasion of lung squamous cell carcinoma

Basic Medicine | 更新时间：2026-01-09

- BNC1 promotes cell migration and invasion of lung squamous cell carcinoma
- Journal of Army Medical University Vol. 46, Issue 7, Pages: 678-687(2024)
- 作者机构：
  
  1.400037 重庆，陆军军医大学(第三军医大学)第二附属医院肿瘤科
  2.400038 重庆，陆军军医大学(第三军医大学)第一附属医院全科医学科
- 作者简介：
- 基金信息：
  
  Supported by the General Program of National Natural Science Foundation of China(81773245)
- DOI：10.16016/j.2097-0927.202312131
  CLC： R73-37;R730.23;R734.2
- Received：28 December 2023，
  
  Revised：2024-02-02，
  
  Published：15 April 2024
- 稿件说明：
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Longyao ZHANG, Longjin ZENG, Linpeng ZHENG, et al. BNC1 promotes cell migration and invasion of lung squamous cell carcinoma[J]. Journal of Army Medical University, 2024, 46(7): 678-687.
DOI：

Longyao ZHANG, Longjin ZENG, Linpeng ZHENG, et al. BNC1 promotes cell migration and invasion of lung squamous cell carcinoma[J]. Journal of Army Medical University, 2024, 46(7): 678-687. DOI： 10.16016/j.2097-0927.202312131.

摘要

目的

探索碱性核蛋白1(

basonuclin

BNC

1)基因对肺鳞状细胞癌细胞迁移和侵袭能力的影响及其作用机制。

方法

利用生物信息学分析在线数据库中

BNC

1基因在肺鳞状细胞癌患者中的表达规律及对生存的影响；采用免疫组织化学对组织芯片进行染色并分析BNC1的蛋白水平；在癌症细胞系百科全书(Cancer Cell Line Encyclopedia，CCLE)数据库中筛选

BNC

1高表达和低表达的肺鳞状细胞癌细胞系；对敲低和过表达的肺鳞状细胞癌细胞H226、SK-MES1进行划痕愈合实验、Transwell迁移和侵袭实验，检测细胞的迁移与侵袭能力的变化；采用RT-qPCR验证10个文献来源的有关迁移与侵袭高度相关的基因；采用Western blot对筛选的调控基因进一步验证。

结果

生物信息学分析发现

BNC

1高表达于肺鳞状细胞癌组织且与较低生存率相关(

=0.047)；肺鳞状细胞癌组织中

BNC

1的表达高于癌旁组织(

0.05)；H226细胞系

BNC

1表达较高，而SK-MES1细胞系

BNC

1表达较低；敲低

BNC

1能够抑制肺鳞状细胞癌细胞的迁移和侵袭(

0.05)，过表达

BNC

1可增强肺鳞状细胞癌细胞的迁移和侵袭(

0.05)；在mRNA层面筛选

BNC

1下游调控分子，发现E-钙粘蛋白(E-cadherin

CDH

1)在

BNC

1过表达时减低(

0.05)，在

BNC

1敲低时升高(

0.05)，Western blot验证结果与mRNA表达一致(

0.05)。

结论

BNC

1可能是肺鳞状细胞癌患者的预后不良基因，

BNC

1基因可能通过下调

CDH

1的表达，促进肺鳞状细胞癌细胞的迁移和侵袭。

Abstract

Objective

To explore the mechanism and effects of

Basonuclin

BNC

1) on the migration and invasion abilities of lung squamous cell carcinoma.

Methods

Bioinformatics technology was used to analyze the expression patterns and survival impact of

BNC

1 in patients with lung squamous cell carcinoma in online databases; Tissue microarrays were stained by immunohistochemistry assay and analyzed for BNC1 protein level. Cell lines for lung squamous cell carcinoma with high and low

BNC

1 expression were screened in the Cancer Cell Line

Encyclopedia (CCLE) database. Wound healing experiment and transwell assay were performed on lung squamous cell carcinoma cell models with knockdown and overexpression of

BNC

and the migration and invasion rates of each cell line were detected; Quantitative reverse transcription PCR(RT-qPCR) was used to validate the migration and invasion of 10 highly related genes derived from literature. Western blotting was used to validate the selected regulatory genes.

Results

Bioinformatics technology found that

BNC

1 was highly expressed in lung squamous cell carcinoma tissues and was associated with lower survival rate(

=0.047). The expression of

BNC

1 in lung squamous cell carcinoma was higher than that in paracancerous tissues (

0.05).

BNC

1 had higher expression in the H226 cells and lower expression in the SK-MES1 cells.

BNC

1 knockdown inhibited the migration and invasion abilities of lung squamous cell carcinoma cells(

0.05)

and

BNC

1 overexpression enhanced the migration and invasion abilities of lung squamous cell carcinoma cells(

0.05). Screening of

BNC

1 downstream regulatory molecules at the mRNA level showed that

CDH

1 was decreased when

BNC

1 was overexpressed (

0.05)

and increased when

BNC

1 was knocked down (

0.05). The results of mRNA level were consistent with the results verified by Western blotting (

0.05).

Conclusion

BNC

1 may be a gene predicting poor prognosis for lung squamous cell carcinoma patients

and

BNC

1 may likely to promote cell migration and invasion of lung squamous cell carcinoma by downregulating

CDH

1 expression.

关键词

Keywords

references

SIEGEL R L, MILLER K D, WAGLE N S, et al . Cancer statistics, 2023[J ] . CA Cancer J Clin, 2023, 73: 17-48. DOI: 10.3322/caac.21763.

PAN L, MO R, ZHU L H, et al . Time trend of mediastinal lymph node dissection in stage IA non-small cell lung cancer patient who undergo lobectomy: a retrospective study of surveillance, epidemiology, and end results (SEER) database[J ] . J Cardiothorac Surg, 2020, 15(1): 207. DOI: 10.1186/s13019-020-01215-x.

XU F, LIN H, HE P, et al . A TP 53-associated gene signature for prediction of prognosis and therapeutic responses in lung squamous cell ca rcinoma[J ] . Oncoimmunology, 2020, 9(1): 1731943. DOI: 10.1080/2162402X.2020.1731943..

THAI A A, SOLOMON B J, SEQUIST L V, et al . Lung cancer[J ] . Lancet, 2021, 398(10299): 535-554. DOI: 10.1016/S0140-6736(21)00312-3.

NING J, WANG F, ZHU K, et al . Characterizing the copy number variation of non-coding RNAs reveals potential therapeutic targets and prognostic markers of LUSC[J ] . Front Genet, 2021, 12: 779155. DOI: 10.3389/fgene.2021.779155.

HIRSCH F R, SCAGLIOTTI G V, MULSHINE JL, et al . Lung cancer: current therapies and new targeted treatments[J ] . Lancet, 2017, 389(10066): 299-311. DOI: 10.1016/S0140-6736(16)30958-8.

ZHAO J G, BAO W M, CAI W Y. Immune infiltration landscape in lung squamous cell carcinoma implications[J]. BioMed Res Int, 2020, 2020: 5981870. DOI: 10.1155/2020/5981870.

HERBST R S, MORGENSZTERN D, BOSHOFF C. The biology and management of non-small cell lung cancer[J]. Nature, 2018, 553(7689): 446-454. DOI: 10.1038/nature25183.

YUAN H, LIU J, ZHANG J. The current landscape of immune checkpoint blockade in metastatic lung squamous cell carcinoma[J]. Molecules, 2021, 26(5): 1392. DOI: 10.3390/molecules26051392.

CUI L, LI H, HUI W, et al . A deep learning-based framework for lung cancer survival analysis with biomarker interpretation[J ] . BMC Bioinformatics, 2020, 21(1): 112. DOI: 10.1186/s12859-020-3431-z.

TEUMER J, TSENG H, GREEN H. The human basonuclin gene[J]. Gene, 1997, 188(1): 1-7. DOI: 10.1016/s0378-1119(96)00659-2.

TSENG H, GREEN H. Association of basonuclin with ability of keratinocytes to multiply and with absence of terminal differentiation[J]. J Cell Biol, 1994, 126(2): 495-506. DOI: 10.1083/jcb.126.2.495.

MAHONEY M G, TANG W, XIANG M M, et al . Translocation of the zinc finger protein basonuclin from the mouse germ cell nucleus to the midpiece of the spermatozoon during spermiogenesis[J ] . Biol Reprod, 1998, 59(2): 388-394. DOI: 10.1095/biolreprod59.2.388.

GAO Y B, LI J Y, JIAO J, et al . Upregulation of Basonuclin1 is associa ted with p63-involved epithelial barrier impairment and type-2 helper T-cell inflammation in chronic rhinosinusitis with nasal polyps[J ] . Int Arch Allergy Immunol, 2021, 182(11): 1046-1057. DOI: 10.1159/000516810.

FEUERBORN A, MATHOW D, SRIVASTAVA P K, et al . Basonuclin-1 modulates epithelial plasticity and TGF-β1-induced loss of epithelial cell integrity[J ] . Oncogene, 2015, 34(9): 1185-1195. DOI: 10.1038/onc.2014.54.

ATABIEKOV I, HOBEIKA E, SHEIKH U, et al . The role of gene therapy in premature ovarian insufficiency management[J ] . Biomedicines, 2018, 6(4): 102. DOI: 10.3390/biomedicines6040102.

GARCÍA-DÍEZ I, HERNÁNDEZ-MUÑOZ I, HERNÁNDEZ-RUIZ E, et al . Transcriptome and cytogenetic profiling analysis of matched in situ/invasive cutaneous squamous cell carcinomas from immunocompetent patients[J ] . Genes Chromosom Cancer, 2019, 58(3): 164-174. DOI: 10.1002/gcc.22712.

GHEORGHE G, BUNGAU S, ILIE M, et al . Early diagnosis of pancreatic cancer: the key for survival[J ] . Diagnostics, 2020, 10(11): 869. DOI: 10.3390/diagnostics10110869.

BISWAS K H. Molecular mobility-mediated regulation of E-cadherin adhesion[J]. Trends Biochem Sci, 2020, 45(2): 163-173. DOI: 10.1016/j.tibs.2019.10.012.

BÜCKER L, LEHMANN U. CDH 1 ( E - cadherin ) gene methylation in human breast cancer: critical appraisal of a long and twisted story[J ] . Cancers, 2022, 14(18): 4377. DOI: 10.3390/cancers14184377..

RÄTZE M A K, KOORMAN T, SIJNESAEL T, et al . Loss of E-cadherin leads to Id2-dependent inhibition of cell cycle progression in metastatic lobular breast cancer[J ] . Oncogene, 2022, 41(21): 2932-2944. DOI: 10.1038/s41388-022-02314-w.

PETROVA Y I, SCHECTERSON L, GUMBINER B M. Roles for E-cadherin cell surface regulation in cancer[J]. Mol Biol Cell, 2016, 27(21): 3233-3244. DOI: 10.1091/mbc.E16-01-0058.

SHENOY S. CDH 1 (E-cadherin) mutation and gastric cancer: genetics, molecular mechanisms and guidelines for management[J ] . Cancer Manag Res, 2019, 11: 10477-10486. DOI: 10.2147/CMAR.S208818.

KUNIDA K, TAKAGI N, AOKI K, et al . Decoding cellular deformation from pseudo-simultaneously observed Rho GTPase ac tivities[J ] . Cell Rep, 2023, 42(2): 112071. DOI: 10.1016/j.celrep.2023.112071.

EBRAHIMI N, KHARAZMI K, GHANAATIAN M, et al . Role of the Wnt and GTPase pathways in breast cancer tumorigenesis and treatment[J ] . Cytokine Growth Factor Rev, 2022, 67: 11-24. DOI: 10.1016/j.cytogfr.2022.05.001.

HE W Z, XU L L, DING J Y, et al . Co-targeting of ACK1 and KIT triggers additive anti-proliferative and-migration effects in imatinib-resistant gastrointestinal stromal tumors[J ] . Biochim Biophys Acta Mol Basis Dis, 2023, 1869(5): 166690. DOI: 10.1016/j.bbadis.2023.166690.

JIANG Y F, LIU Y R, ZHANG Y Y, et al . MicroRNA-142-3P suppresses the progression of papillary thyroid carcinoma by targeting FN1 and inactivating FAK/ERK/PI3K signaling[J ] . Cell Signal, 2023, 109: 110792. DOI: 10.1016/j.cellsig.2023.110792.

BENTON D, CHERNOFF J. RHOA drivers take alternate routes in gastric cancer[J]. Sci Signal, 2023, 16(816): eadk9171. DOI: 10.1126/scisignal.adk9171.

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⁰

BNC1 promotes cell migration and invasion of lung squamous cell carcinoma

DOI：10.16016/j.2097-0927.202312131

摘要

Abstract

关键词

Keywords

references