Effect of <italic style="font-style: italic">Lactobacillus casei</italic> on peripheral and central nervous inflammation in depressed offspring rats induced by maternal separation

Yurong ZHANG; Siqi Tan; Ruizhong WANG; Li WANG; Rui CHEN; Rui XU

doi:10.16016/j.2097-0927.202312115

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Effect of Lactobacillus casei on peripheral and central nervous inflammation in depressed offspring rats induced by maternal separation

Basic Medicine | 更新时间：2026-01-09

- Effect of Lactobacillus casei on peripheral and central nervous inflammation in depressed offspring rats induced by maternal separation
- Journal of Army Medical University Vol. 46, Issue 16, Pages: 1867-1873(2024)
- 作者机构：
  
  1.710077 西安，西安医学院第一附属医院科研科
  2.710000 西安，西安医学院全科医学院
  3.710077 西安，西安医学院第一附属医院妇科
- 作者简介：
- 基金信息：
  
  the Project of Science and Technology Plan of Xi'an Bureau of Science and Technology(23YXYJ0163);the Project of Provincial College Students' Innovation and Entrepreneurship Training Program(S202311840061);the Scientific Research Project From the First Affiliated Hospital of Xi'an Medical University(XYYFY-2023-01);the General Project of Shaanxi Provincial Department of Science and Technology-Social Development Field(2024SF-YBXM-077)
- DOI：10.16016/j.2097-0927.202312115
  CLC： Q939.117;R338.26;R749.4
- Received：23 December 2023，
  
  Revised：2024-03-05，
  
  Published：30 August 2024
- 稿件说明：
移动端阅览
Yurong ZHANG, Siqi Tan, Ruizhong WANG, et al. Effect of Lactobacillus casei on peripheral and central nervous inflammation in depressed offspring rats induced by maternal separation[J]. Journal of Army Medical University, 2024, 46(16): 1867-1873.
DOI：

Yurong ZHANG, Siqi Tan, Ruizhong WANG, et al. Effect of Lactobacillus casei on peripheral and central nervous inflammation in depressed offspring rats induced by maternal separation[J]. Journal of Army Medical University, 2024, 46(16): 1867-1873. DOI： 10.16016/j.2097-0927.202312115.

摘要

目的

观察干酪乳杆菌对产后进行母婴分离(maternal separation

MS)诱导子代大鼠抑郁样行为的影响

探索益生菌改善抑郁样行为潜在的中枢神经炎症的作用机制。

方法

采用母婴分离应激方法建立子代抑郁动物模型

并将SD子鼠采用随机数字表法分为对照组(CON组)、母婴分离组(MS组)、母婴分离+干酪乳杆菌组(MS+干酪乳杆菌组)

每组8只。其中对照组雌鼠在产后与子鼠不进行母婴分离干预; 母婴分离组雌鼠在正常分娩后与子鼠每天分离3 h

连续分离14 d; MS+干酪乳杆菌组的子鼠在MS组基础上给予为期4周的干酪乳杆菌干预

每天按体质量灌胃8×10

CFU/(kg·d)。采用糖水偏好实验(SPT)和强迫游泳实验(FST)评估各组子代大鼠的抑郁样行为

采用ELISA法测定各组子代大鼠血清和海马组织中TNF-α、IL-6、IL-1 β、IL-10的水平

采用荧光实时定量PCR法检测各组子代大鼠海马组织中IDO1及5-HT mRNA的表达水平。

结果

与对照组相比

母婴分离组子代大鼠的糖水偏好百分比明显降低(

0.05)

虽然自发活动实验中穿格次数的减少差异无统计学意义

但强迫游泳实验中的不动时间显著延长(

0.05)

说明母婴分离组子代大鼠产生了明显的抑郁样行为。而干酪乳杆菌显著改善了母婴分离应激引起的子代大鼠的行为学变化

明显提高了糖水偏好百分比(

0.05)

缩短了强迫游泳的不动时间(

0.05)。与MS组相比

MS+干酪乳杆菌组子代大鼠海马和外周血中IL-6、IL-1 β、TNF-α的含量显著降低(

0.01)

IL-10的含量显著增加(

0.05);子代大鼠海马中IDO1 mRNA的表达显著降低(

0.01)

5-HT mRNA的表达显著升高(

0.01)。

结论

母婴分离诱导子代大鼠产生了抑郁样行为

干酪乳杆菌通过调节外周血和海马炎性因子的表达

抑制海马中IDO1的表达、增加海马中5-HT的表达

可改善子代大鼠抑郁样行为。

Abstract

Objective

To observe the effect of

Lactobacillus casei

(

L.casei

) on the depression-like behaviors of offspring rats induced by maternal separation (MS)

and explore the potential action mechanism of probiotics to improve central nervous inflammation in depression-like behaviors.

Methods

MS stress was used to establish a depressed animal model in offspring.Female SD rats were randomly divided into control group (CON)

MS group

and MS+

L.casei

group

with 8 animals in each group.The female rats and their offspring in the control group did not undergo MS intervention after delivery

while those in the MS group were separated 3 h each day

for 14 consecutive days after normal delivery.The offspring of the MS+

L.casei

group was given intervention of 8×10

CFU/(kg·d)

L.casei

gava

ge for 4 weeks.Source preference test (SPT) and forced swimming test (FST) were used to evaluate the changes in depressive behaviors of the offspring rats.ELISA was employed to determine the levels of TNF-α

IL-6

IL-1 β and IL-10 in serum and hippocampus tissue.Real-time fluorescence quantitative PCR was applied to detect the mRNA expression levels of indoleamine 2

3-dioxygenase-1(IDO1) and 5-HT mRNA in hippocampus.

Results

Compared with the CON group

the offspring in the MS group had significantly reduced percentage of source preference (

0.05)

prolonged immobility time during forced swimming (

0.05)

and decreased number of grid crossings in spontaneous activity test (though no statistical difference)

indicating that the offspring rats had obvious depression-like behaviors after MS.

L.casei

treatment improved the behavioral changes of offspring rats caused by MS stress

with significantly increased percentage of source preference (

0.05) and shortened immobility time of forced swimming (

0.05).Compared with the conditions in the MS group

L.casei

reduced the levels of IL-6

IL-1 β and TNF-α(

0.01) and increased that of IL-10(

0.05) in the hippocampus and peripheral blood

and decreased mRNA level of IDO1 and enhanced 5-HT mRNA level in hippocampus (

0.01) in the offspring rats of the MS+

L.casei

group.

Conclusion

MS stress induces depression-like behavior in offspring rats.

L.casei

inhibits the hippocampal expression of IDO1 and enhances that of 5-HT by regulating inflammatory factors in hippocampus and peripheral blood

and thus

improves depression-like behavior.

关键词

Keywords

references

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⁰

Effect of Lactobacillus casei on peripheral and central nervous inflammation in depressed offspring rats induced by maternal separation

DOI：10.16016/j.2097-0927.202312115

摘要

Abstract

关键词

Keywords

references