Enamel matrix derivative improves remineralization of artificial root dentin caries <italic style="font-style: italic">in vitro</italic>

Jin CHEN; Jing'ou LIANG; Linglin ZHANG

doi:10.16016/j.2097-0927.202312108

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Enamel matrix derivative improves remineralization of artificial root dentin caries in vitro

Biomedical Engineering | 更新时间：2026-01-09

- Enamel matrix derivative improves remineralization of artificial root dentin caries in vitro
- Journal of Army Medical University Vol. 46, Issue 16, Pages: 1890-1896(2024)
- 作者机构：
  
  1.401120 重庆，重庆医科大学附属口腔医院附三院门诊部
  2.401147 重庆，重庆市口腔疾病与生物医学重点实验室，重庆市高校市级口腔生物医学工程重点实验室
  3.610041，成都，四川大学华西口腔医院儿童口腔科
  4.610041，成都，四川大学华西口腔医院牙体牙髓科
  5.610041，成都，四川大学华西口腔医院口腔疾病防治全国重点实验室，国家口腔医学中心，国家口腔疾病临床医学研究中心
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81271128)
- DOI：10.16016/j.2097-0927.202312108
  CLC： R780.2;R781.1;R977.6
- Received：21 December 2023，
  
  Revised：2024-04-09，
  
  Published：30 August 2024
- 稿件说明：
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Jin CHEN, Jing'ou LIANG, Linglin ZHANG. Enamel matrix derivative improves remineralization of artificial root dentin caries in vitro[J]. Journal of Army Medical University, 2024, 46(16): 1890-1896.
DOI：

Jin CHEN, Jing'ou LIANG, Linglin ZHANG. Enamel matrix derivative improves remineralization of artificial root dentin caries in vitro[J]. Journal of Army Medical University, 2024, 46(16): 1890-1896. DOI： 10.16016/j.2097-0927.202312108.

摘要

目的

探讨牙釉基质衍生物(enamel matrix derivative

EMD)对人工根面牙本质龋再矿化作用的影响。

方法

将牛牙根面牙本质样本放置在脱矿溶液中，形成人工龋损样本，采用简单随机抽样法分为EMD组(30 g/L EMD，实验组)、氟化钠(sodium fluoride

NaF)组(1 g/L NaF，阳性对照组)和去离子水(distilled and deionised water

DDW)组(阴性对照组)，进行pH循环处理8 d，每天6次。使用扫描电子显微镜(scanning electron microscope

SEM)及偏振光显微镜(polarized light microscopy

PLM)观察各组处理前后龋损形态学的变化情况，并使用横断显微照相技术(transverse microradiography

TMR)检测处理前后矿物质丢失量、龋损深度和矿物含量的变化情况。

结果

pH循环处理后，SEM的表面形态学图像和PLM的垂直形态学图像均显示，EMD组和NaF组人工根面牙本质龋的矿物质沉积增加，而DDW组无矿物质沉积。同时，TMR结果显示，与DDW组相比，EMD组矿物丢失较低，龋损深度较浅，矿物含量较高(

0.05)。

结论

釉基质衍生物能促进根面牙本质龋的再矿化，具有成为一种有效的根面无创防龋制剂的潜力。

Abstract

Objective

To determine the effect of enamel matrix derivative (EMD) on remineralization of artificial root dentin caries.

Methods

Bovine root dentin blocks were placed in a demineralization solution to create artificial root dentin carious lesions. The lesions were then randomly divided into EMD group (30 g/L EMD

the experimental group)

sodium fluoride (NaF) aqueous solution group (1 g/L NaF

the positive control group) and distilled and deionised water (DDW) group (the negative control group)

and all the lesions were pH-cycled at 6 cycles per day for 8 d. Morphology of the lesions were observed by scanning electron microscopy (SEM) and polarized light microscopy (PLM). Transverse microradiography (TMR) was used to examine mineral loss

lesion depth and mineral content.

Results

After pH-cycling

the surface morphology by SEM and the vertical morphology by PLM suggested that EMD and NaF treatment

but not DDW

caused mineral accumulation of artificial root dentin caries. Meanwhile

TMR revealed significantly lower mineral loss

shallower lesion depth and more mineral content in the EMD group than the DDW group (

0.05) after pH-cycling.

Conclusion

EMD can significantly promote remineralization of root dentin caries

in vitro

and is a promising agent for non-invasive root caries therapy.

关键词

Keywords

references

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Enamel matrix derivative improves remineralization of artificial root dentin caries in vitro

DOI：10.16016/j.2097-0927.202312108

摘要

Abstract

关键词

Keywords

references