Screening of evaluation indicators for motor skeletal muscle injury in rats in hot and dry desert environment

Tangxiao WU; Xi YANG; Ziyi YIN; Yingqiu WU; Jili WU; Huaqiang HUI

doi:10.16016/j.2097-0927.202311060

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Screening of evaluation indicators for motor skeletal muscle injury in rats in hot and dry desert environment

Military Medicine | 更新时间：2026-01-09

- Screening of evaluation indicators for motor skeletal muscle injury in rats in hot and dry desert environment
- Journal of Army Medical University Vol. 46, Issue 8, Pages: 894-900(2024)
- 作者机构：
  
  1.830011 乌鲁木齐市，新疆医科大学公共卫生学院
  2.830000 乌鲁木齐市，新疆军区总医院科研教学科
  3.830000 乌鲁木齐市，新疆军区总医院卫勤部
  4.832061 新疆维吾尔自治区石河子市，石河子大学医学院预防医学系
- 作者简介：
- 基金信息：
  
  the Special Project of Regional Collaborative Innovation of Xinjiang Uygur Autonomous Region(科技援疆计划);the Special Project of Regional Collaborative Innovation of Xinjiang Uygur Autonomous Region(2022E02121);the Project of Postgraduate Scientific Research and Innovation of Xinjiang Uygur Autonomous Region(XJ2023G190)
- DOI：10.16016/j.2097-0927.202311060
  CLC： R873.4;R122.2+1; R122.2+3; R685.4
- Received：13 November 2023，
  
  Revised：2024-03-13，
  
  Published：30 April 2024
- 稿件说明：
移动端阅览
Tangxiao WU, Xi YANG, Ziyi YIN, et al. Screening of evaluation indicators for motor skeletal muscle injury in rats in hot and dry desert environment[J]. Journal of Army Medical University, 2024, 46(8): 894-900.
DOI：

Tangxiao WU, Xi YANG, Ziyi YIN, et al. Screening of evaluation indicators for motor skeletal muscle injury in rats in hot and dry desert environment[J]. Journal of Army Medical University, 2024, 46(8): 894-900. DOI： 10.16016/j.2097-0927.202311060.

摘要

目的

使用西北特殊环境人工实验舱建立沙漠干热环境大鼠运动骨骼肌损伤模型

探讨沙漠干热环境下大鼠运动骨骼肌损伤的组织形态学和骨骼肌损伤相关等指标

筛选干热环境运动骨骼肌损伤评价指标。

方法

18只8周龄雄性SD大鼠按随机数字表法分为(

=6): 空白对照组(常温环境中自由运动)、常温运动组(常温环境中力竭运动)、干热运动组(干热环境中力竭运动)。3组大鼠先进行3 d的运动跑台适应性训练(速度为16 m/min

坡度为-16°

时间为5~10 min); 常温运动组和干热运动组大鼠以19.3 m/min速度、-16°坡度运动至力竭结束

1次/d; 干热运动组采用西北特殊环境人工实验舱模拟沙漠干热环境[温度(40±1)℃

湿度(10±2)%

]

建立模型。实验连续6 d

取大鼠比目鱼肌组织观察病理学变化

通过血清学检测大鼠骨骼肌损伤标志物、炎症因子、氧化应激、激素等相关指标。

结果

干热运动组骨骼肌损伤标志物肌酸激酶(concentrations of creatine kinase

CK)、肌酸激酶同工酶(creatine kinase isoenzyme

CK-MB)、肌型肌酸激酶(muscle-specific creatine kinase

CK-MM)的浓度均高于空白对照组、常温运动组(

0.05);干热运动组炎症因子肿瘤坏死因子-α(tumor necrosis factor-α

TNF-α)、白介素-1β(interleukin-1β

IL-1β)、IL-6的浓度均高于空白对照组、常温运动组(

0.05);干热运动组氧化应激丙二醛(malondialdehyde

MDA)的浓度均高于空白对照组、常温运动组(

0.05);干热运动组超氧化物歧化酶(superoxide dismutase

SOD)的浓度低于空白对照组、常温运动组(

0.05);干热运动组睾酮(testosterone

T)的浓度均低于空白对照组、常温运动组(

0.05);干热运动组皮质酮(corticosterone

Cort)的浓度高于空白对照组、常温运动组(

0.05);干热运动组睾酮/皮质酮(testosterone/corticosterone

T/Cort)的浓度低于空白对照组、常温运动组(

0.05);骨骼肌损伤标志物CK、CK-MB和CK-MM与促炎症细胞因子TNF-α、IL-1β和IL-6呈正相关(

0.05);与MDA呈正相关

与抗氧化指标SOD呈负相关(

0.05)、与激素水平Cort呈正相关

与T、T/Cort呈负相关(

0.05)。

结论

本研究构建出沙漠干热运动骨骼肌损伤动物模型

验证了沙漠干热环境较常温环境运动骨骼肌损伤程度大

筛选出TNF-α和IL-1β可作为评价损伤的间接指标。

Abstract

Objective

To explore the histomorphology and related indicators in a rat model of skeletal muscle injury in an artificial experimental chamber as special environment in Northwest China to stimulate a hot and dry desert environment in order to screen the evaluation indicators of skeletal muscle injury in a hot and dry environment.

Methods

Eighteen male SD rats (8 weeks old) were randomly divided into blank control group (room temperature

free movement)

room temperature exercise group

and hot and dry exercise group

with 6 rats in each group.After the rats were subjected to adaptation training for 3 d (a 3-D exercise treadmill

speed: 16 m/min

slope: -16°

time: 5~10 min)

the rats in the 2 exercise groups were subjected to a treadmill once per day at a speed of 19.3 m/min and a slope of-16° till the end of exhaustion

and those in the hot and dry exercise group were housed in an artificial experimental chamber at a temperature of (40±1)℃

a humidity of (10±2)%.After 6 d of continuous exercise

the rat soleus muscle tissue was taken to observe the pathological changes

and the serum samples were detected for skeletal muscle injury markers

inflammatory factors

oxidative stress related factors

hormones and other related indicators.

Results

When compared with the blank control group and the room temperature exercise group

the hot and dry exercise group had significantly higher concentrations of creatine kinase (CK)

creatine kinase isoenzyme (CK-MB) and myotype creatine kinase (CK-MM)(

0.05)

elevated contents of TNF-α

IL-1β and IL-6(

0.05)

raised malondialdehyde (MDA) content and lower superoxide dismutase (SOD) activity (

0.05)

lower testosterone (T) but higher corticosterone (Cort) levels and thus lower testosterone/corticosterone (T/Cort) ratio (

0.05).The markers of skeletal muscle injury

CK-MB and CK-MM were positively correlated with the pro-inflammatory cytokines

TNF-α

IL-1β and IL-6(

0.05)

positively with the antioxidant indicator MDA

but negatively with SOD (

0.05)

and positively correlated with Cort level and negatively with T level and T/Cort ratio (

0.05).

Conclusion

A stable rat model of skeletal muscle injury in a hot and dry desert environment is successfully constructed.The severity of skeletal muscle injury is greater in rats in a hot and dry desert environment than those in a normal temperature environment.TNF-α and IL-1β are found to be indirect indicators to evaluate skeletal muscle injury in the environment.

关键词

Keywords

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