Oxygen-glucose deprivation preconditioning alleviates oxygen-glucose deprivation/reperfusion injury in PC12 cells by protecting mitochondria

Li HUANG; Na XU; Chaoxian YANG

doi:10.16016/j.2097-0927.202311019

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Oxygen-glucose deprivation preconditioning alleviates oxygen-glucose deprivation/reperfusion injury in PC12 cells by protecting mitochondria

Basic Medicine | 更新时间：2026-01-09

- Oxygen-glucose deprivation preconditioning alleviates oxygen-glucose deprivation/reperfusion injury in PC12 cells by protecting mitochondria
- Journal of Army Medical University Vol. 46, Issue 15, Pages: 1763-1771(2024)
- 作者机构：
  
  1.400037 重庆，陆军军医大学(第三军医大学)第二附属医院麻醉科
  2.646000 四川泸州，西南医科大学基础医学院解剖教研室
- 作者简介：
- 基金信息：
  
  the Natural Science Foundation of Sichuan Province(2022NSFSC0718);the General Project of Applied Basic Medicine of Southwest Medical University(2021ZKMS011)
- DOI：10.16016/j.2097-0927.202311019
  CLC： R329.25;R363.22;R741.02
- Received：05 November 2023，
  
  Revised：2023-12-06，
  
  Published：15 August 2024
- 稿件说明：
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Li HUANG, Na XU, Chaoxian YANG. Oxygen-glucose deprivation preconditioning alleviates oxygen-glucose deprivation/reperfusion injury in PC12 cells by protecting mitochondria[J]. Journal of Army Medical University, 2024, 46(15): 1763-1771.
DOI：

Li HUANG, Na XU, Chaoxian YANG. Oxygen-glucose deprivation preconditioning alleviates oxygen-glucose deprivation/reperfusion injury in PC12 cells by protecting mitochondria[J]. Journal of Army Medical University, 2024, 46(15): 1763-1771. DOI： 10.16016/j.2097-0927.202311019.

摘要

目的

研究氧糖剥离预处理(oxygen-glucose deprivation preconditioning，OGDPC)对氧糖剥离/再灌注(oxygen-glucose deprivation/reperfusion

OGD/R)损伤PC12细胞的保护作用及机制。

方法

简单随机抽样将PC12细胞分为Control、OGDPC、OGD/R和OGDPC+OGD/R共4组。透射电镜观察线粒体的超微结构，Mito-tracker染色观察线粒体数量，Western blot检测OGDH、PDHA1、COX4、HSP60的表达，JC-1和ATP试剂盒评估线粒体膜电位及ATP水平，TUNEL染色观察细胞凋亡情况，并与AIF、EndoG的免疫荧光染色进行共定位。

结果

与OGD/R组相比，OGDPC+OGD/R组PC12细胞线粒体超微结构损伤较轻；OGDH、PDHA1和COX4的表达增多(

0.05)，HSP60的表达减少(

0.05)；线粒体膜电位及ATP水平提高(

0.05)，细胞凋亡率降低(

0.05)，且AIF、EndoG向TUNEL染色阳性的细胞核聚集减少(

0.05)。

结论

OGDPC减轻OGD/R引起的PC12细胞损伤，其机制可能与改善线粒体结构和功能、减少线粒体途径引起的细胞凋亡有关。

Abstract

Objective

To investigate the protective effect of oxygen-glucose deprivation preconditioning (OGDPC) on PC12 cells damaged with oxygen-glucose deprivation/reperfusion (OGD/R) and its underlying mechanism.

Methods

PC12 cells were randomly divided into Control

OGDPC

OGD/R and OGDPC+OGD/R groups. The morphology and quantity of mitochondria were observed by transmission electron microscopy (TEM) and Mito-tracker staining. The expression of OGDH

PDHA1

COX4 and HSP60 was detected by Western blotting. Mitochondrial membrane potential and ATP production were evaluated by JC-1 and ATP assay kit. TUNEL staining was used to assess cell apoptosis

and translocation of AIF and EnodoG was observed by immunofluorescence staining and TUNEL staining.

Results

Compared with the OGD/R group

the mitochondrial ultrast

ructure damage were alleviated

the expression levels of OGDH

PDHA1 and COX4 were enhanced (

0.05)

that of HSP60 was decreased (

0.05)

the mitochondrial membrane potential and ATP level were increased (

0.05)

the cell apoptotic rate was reduced (

0.05)

and AIF and EndoG were less aggregated to the TUNEL-positive nucleus in OGDPC+OGD/R group (

0.05).

Conclusion

OGDPC can alleviate the injury of PC12 cells caused by OGD/R

and the potential mechanism may be related to improving mitochondrial structure and function

and reducing cell apoptosis

via

mitochondrial pathway.

关键词

Keywords

references

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