Association of intestinal and oral microbiota with acute graft versus host disease of the gastrointestinal tract in patients after allogeneic hematopoietic stem cell transplantation

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Association of intestinal and oral microbiota with acute graft versus host disease of the gastrointestinal tract in patients after allogeneic hematopoietic stem cell transplantation

Monographic Reports: Advances in diagnosis and treatment for hematological malignancies | 更新时间：2026-01-09

- Association of intestinal and oral microbiota with acute graft versus host disease of the gastrointestinal tract in patients after allogeneic hematopoietic stem cell transplantation
- Journal of Army Medical University Vol. 46, Issue 4, Pages: 331-339(2024)
- 作者机构：
  
  1.610072 成都，四川省医学科学院·四川省人民医院(电子科技大学医学院)血液内科
  2.310012 杭州，浙江省立同德医院检验科
- 作者简介：
- 基金信息：
  
  the Technology Innovation Project of Chengdu Science and Technology Bureau(2019-YF05-00638-S1V)
- DOI：10.16016/j.2097-0927.202310041
  CLC： R372;R392.8;R574
- Received：12 October 2023，
  
  Revised：2024-01-02，
  
  Published：28 February 2024
- 稿件说明：
移动端阅览
Jing LAI, Xin FU, Chenglong LI, et al. Association of intestinal and oral microbiota with acute graft versus host disease of the gastrointestinal tract in patients after allogeneic hematopoietic stem cell transplantation[J]. Journal of Army Medical University, 2024, 46(4): 331-339.
DOI：

Jing LAI, Xin FU, Chenglong LI, et al. Association of intestinal and oral microbiota with acute graft versus host disease of the gastrointestinal tract in patients after allogeneic hematopoietic stem cell transplantation[J]. Journal of Army Medical University, 2024, 46(4): 331-339. DOI： 10.16016/j.2097-0927.202310041.

摘要

目的

2

初步探讨异基因造血干细胞移植(allogeneic hematopoietic stem cell transplantation

allo-HSCT)患者肠道菌群及口腔菌群的变化与早期胃肠道急性移植物抗宿主病(acute graft versus host disease

aGVHD)之间的关联

寻找可能有效的生物学标志物。

方法

2

选取2021年9月至2023年6月在四川省人民医院血液内科接受allo-HSCT后1个月内发生胃肠道aGVHD的10例急性白血病患者

收集其aGVHD前后粪便样本与唾液样本

采用16S rRNA测序分析方法

研究早期胃肠道aGVHD发生前后肠道菌群、口腔菌群的差异性改变。

结果

2

① 肠道菌群中拟杆菌属减少、肠球菌属及肠杆菌科增加与早期上消化道aGVHD发生呈正相关(

P

<

0.05)

而口腔菌群整体微生物多样性无显著差异(

P

>

0.05)。②胃肠道aGVHD前后肠道菌群LEfSe分析发现克雷伯杆菌属、肠球菌属增加

大肠埃希菌减少; 口腔菌群LEfSe分析发现存在差异显著10个微生物标志物

其中γ-变形菌纲的差异较为显著。③肠道菌群β多样性差异显著(

P

=0.03)

而口腔菌群α、β多样性无显著差异。

结论

2

Allo-HSCT患者发生早期胃肠道aGVHD前后肠道菌群存在显著差异

可能与口腔菌群变化有关。

Abstract

Objective

2

To preliminarily investigate the association between changes in intestinal microbiota and oral microbiota of allogeneic hematopoietic stem cell transplantation (allo-HSCT) patients and early gastrointestinal acute graft versus host disease (aGVHD)

and try to explore potentially effective biomarkers and provide theoretical basis for early prediction and intervention of gastrointestinal aGVHD.

Methods

2

Ten acute leukemia patients who developed gastrointestinal aGVHD within 1 month after receiving allo-HSCT in Department of Hematology of Sichuan Provincial People's Hospital from September 2021 to June 2023 were enrolled

and their fecal samples and saliva samples before and after the aGVHD were collected.16S rRNA sequencing analysis was applied for the differential changes in intestinal and oral microbiota before and after the development of early gastrointestinal aGVHD.

Results

2

① A decrease in

Bacteroides spp

.and an increase in

Enterococcus spp

.and

Enterobacteriaceae spp

.in the intestinal microbiota were positively correlated with the occurrence of early upper gastrointestinal aGVHD (

P

<

0.05)

whereas no significant difference was observed in the overall microbial diversity of the oral microbiota (

P

>

0.05).② LEfSe analysis of the intestinal microbiota before and after gastrointestinal aGVHD revealed an increase in

Klebsiella spp

.and

Enterococcus spp

.and a decrease in

Escherichia coli

; In the oral microbiota

LEfSe analysis revealed 10 microbial markers with significant difference

of which Gamma proteobacteria was the most significant.③ The difference in β-diversity of the intestinal microbiota was significant (

P

=0.03)

whereas there was no significant difference in the α-and β-diversity of the oral microbiota.

Conclusion

2

Significant differences are found in intestinal microbiota before and after the occurrence of early gastrointestinal aGVHD in patients after Allo-HSCT

and the occurrence may have a correlation with the chang in oral microbiota.

关键词

Keywords

references

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