Correlation between intestinal bacteria <italic style="font-style: italic">Phascolarctobacterium</italic>/<italic style="font-style: italic">Phascolarctobacterium faecium</italic> and metabolic disorder in patients with type 2 diabetes mellitus

Lisha LI; Daqian XIONG

doi:10.16016/j.2097-0927.202310036

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Correlation between intestinal bacteria Phascolarctobacterium/Phascolarctobacterium faecium and metabolic disorder in patients with type 2 diabetes mellitus

Clinical Medicine | 更新时间：2026-01-09

- Correlation between intestinal bacteria Phascolarctobacterium/Phascolarctobacterium faecium and metabolic disorder in patients with type 2 diabetes mellitus
- Journal of Army Medical University Vol. 46, Issue 13, Pages: 1561-1568(2024)
- 作者机构：
  
  610072 成都，成都中医药大学附属医院检验科
- 作者简介：
- 基金信息：
  
  the Key Research and Development Project of Science and Technology Plan of Sichuan Province(2020YFS0375);the Special Project for Nursery Talents of Xinglin Scholars Program From Chengdu University of Traditional Chinese Medicine(MPRC2023025)
- DOI：10.16016/j.2097-0927.202310036
  CLC： R364.2;R378.2;R587.1
- Received：10 October 2023，
  
  Revised：2024-01-04，
  
  Published：15 July 2024
- 稿件说明：
移动端阅览
Lisha LI, Daqian XIONG. Correlation between intestinal bacteria Phascolarctobacterium/Phascolarctobacterium faecium and metabolic disorder in patients with type 2 diabetes mellitus[J]. Journal of Army Medical University, 2024, 46(13): 1561-1568.
DOI：

Lisha LI, Daqian XIONG. Correlation between intestinal bacteria Phascolarctobacterium/Phascolarctobacterium faecium and metabolic disorder in patients with type 2 diabetes mellitus[J]. Journal of Army Medical University, 2024, 46(13): 1561-1568. DOI： 10.16016/j.2097-0927.202310036.

摘要

目的

寻找新发2型糖尿病(type 2 diabetes mellitus

T2DM) 中的差异菌群，并初步评估差异核心菌群在疾病代谢紊乱中的重要作用。

方法

选取2020年9-11月在成都中医药大学附属医院内分泌科就诊的30名T2DM患者与体检中心的30名健康者为研究对象，构成T2DM组与对照组(NC组)。收集2组志愿者的血液标本和粪便标本，血液标本用于葡萄糖、胰岛素和血脂类指标的检测，粪便标本用于肠道菌群的16SrRNA检测，之后结合生物信息学分析、Pearson相关性分析等统计学方法，对不同组间的T2DM血液指标和差异菌种进行分析；使用RT-qPCR验证前期小RNA谱筛选结果，并将筛选的差异菌与早期实验的脂质代谢组、miRNAs谱基因组数据进行多组学关联分析，构建多参数的ROC曲线模型。

结果

T2DM具有特异性的肠道菌群特征，考拉杆菌属(

Phascolarctobacterium

)与粪考拉杆菌(

Phascolarctobacterium faecium

P.faecium

)在患病前后差异具有统计学意义。Pearson相关性分析提示，

P.faecium

与高密度脂蛋白(high-density lipoprotein

HDL)(

=-0.78

0.001)、低密度脂蛋白(low-density lipoprotein

LDL)(

=-0.89

0.001)、胰岛素(

=-0.71

0.01)具有较强的负相关性。

Phascolarctobacterium

则与胰岛素(

=-0.67

0.001)、体质量指数(body mass index

BMI) (

=-0.64

0.001)具有一定的负相关性，所构建的基于

Phascolarctobacterium

P.faecium

、脂肪酸α-linolenic acid、

miR

-122-5

的联合指标的ROC曲线模型，能够比较准确地将T2DM患者与健康者区分，曲线下面积(area under curve

AUC)分别为0.928 (

=0.001) 与0.934 (

0.001)。

结论

T2DM患者具有特异性的肠道菌群分布，其中，

Phascolarctobacterium

菌属和

P.faecium

菌与疾病的代谢紊乱密切相关，两类细菌理论上可作为T2DM的核心菌。

Abstract

Objective

To explore the differential microbiota in patients with newly diagnosed type 2 diabetes mellitus (T2DM)

and to preliminarily evaluate the importance of the differential core microbiota in the metabolic disorder.

Methods

A total of 30 T2DM patients who were treated at the Endocrinology Department of our hospital from September to November 2020

and 30 healthy individuals taking physical examination during the same period were enrolled and assigned into the T2DM group and the control group

respectively. Their blood and fecal samples were collected for the detection of glucose

insulin

and lipid indicators levels and for gut microbiota using 16SrRNA

respectively. Subsequently

bioinformatics analysis

Pearson correlation analysis

and other statistical methods were applied to analyze the differences in T2DM blood indicators and specific bacterial species between the 2 groups. RT-qPCR was used to verify the screening results of small RNA profiles. Then a multi-parameter receiver operating characteristic (ROC) curve model was constructed based on the results of correlation analysis including microbiome

lipid metabolome and miRNAs spectrum genomic detection data of early research.

Results

T2DM showed specific characteristics of intestinal microbiota

with

Phascolarctobacterium

and

Phascolarctobacterium. faecium

(

P. faecium

) showing significant differences before and after the onset of the disease. Pearson correlation analysis suggested that

P. faecium

was negatively correlated with high-density lipoprotein (HDL) (

=-0.78

0.001)

low-density lipoprotein (LDL) (

=-0.89

0.001)

and insulin (

=-0.71

0.001). While

Phascolarctobacterium

was negatively correlated with insulin (

=-0.67

0.001) and body mass index (BMI) (

=-0.64

0.001). The ROC curve model based on

Phascolarctobacterium

P. faecium

α-linolenic acid

and miR-122-5p could accurately distinguish between T2DM patients and healthy individuals

with a value of area under the curve of 0.928 (

=0.0001) and 0.934 (

0.0001)

which suggesting core values of 2 types

of bacteria in the disease.

Conclusion

T2DM patients have specific gut microbiota distribution

in which

Phascolarctobacterium

and

P. faecium

are closely associated with metabolic disorders of the disease. The 2 types of bacteria can be regarded as core bacteria for T2DM.

关键词

Keywords

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Correlation between intestinal bacteria Phascolarctobacterium/Phascolarctobacterium faecium and metabolic disorder in patients with type 2 diabetes mellitus

DOI：10.16016/j.2097-0927.202310036

摘要

Abstract

关键词

Keywords

references