Effects of isocaloric high-fat diet on energy metabolism and endurance exercise capacity in SD rats

Shuai CHEN; Xiangyuan DENG; Hedong LANG; Ruiliang ZHANG; Xin RAO; Jiaqi YUAN; Jundong ZHU; Yu QIN; Mantian MI

doi:10.16016/j.2097-0927.202310021

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Effects of isocaloric high-fat diet on energy metabolism and endurance exercise capacity in SD rats

Public Health and Preventive Medicine | 更新时间：2026-01-09

- Effects of isocaloric high-fat diet on energy metabolism and endurance exercise capacity in SD rats
- Journal of Army Medical University Vol. 46, Issue 9, Pages: 940-951(2024)
- 作者机构：
  
  400038 重庆，陆军军医大学(第三军医大学)军事预防医学系营养与食品安全研究中心，重庆市医学营养研究中心，营养与健康重庆市重点实验室
- 作者简介：
- 基金信息：
- DOI：10.16016/j.2097-0927.202310021
  CLC： R151.1;R151.41;R153.4
- Received：07 October 2023，
  
  Revised：2024-01-08，
  
  Published：15 May 2024
- 稿件说明：
移动端阅览
Shuai CHEN, Xiangyuan DENG, Hedong LANG, et al. Effects of isocaloric high-fat diet on energy metabolism and endurance exercise capacity in SD rats[J]. Journal of Army Medical University, 2024, 46(9): 940-951.
DOI：

Shuai CHEN, Xiangyuan DENG, Hedong LANG, et al. Effects of isocaloric high-fat diet on energy metabolism and endurance exercise capacity in SD rats[J]. Journal of Army Medical University, 2024, 46(9): 940-951. DOI： 10.16016/j.2097-0927.202310021.

摘要

目的

探讨等能量的45%高脂饮食对SD大鼠代谢及耐力运动能力的影响。

方法

24只雄性SD大鼠采用随机数字表法分为：常规喂养组(CON组)、高脂喂养组(HFD组)、常规喂养+训练组(CONT组)、高脂喂养+训练组(HFDT组)。其中CON组、CONT组给予常规饲料喂养，HFD组、HFDT组给予等能量的45%高脂饲料喂养；HFDT组和CONT组同时进行中等强度跑步耐力训练，持续6周。每周测量体质量、脂肪体质量和瘦体质量。用Pheno Master/Calo Treadmill系统测量静息和运动状态下的能量消耗和基础代谢率。在大鼠进行力竭测试后，检测血糖、血脂、血清肌酸激酶等指标。

结果

干预6周后，HFDT组耐力运动能力较CONT组增强(

0.05)。等能量喂养的各组大鼠体质量和体成分没有明显差异。静息时，各组大鼠总能量消耗与基础代谢率无明显差异，但在4周以前，CON组以碳水化合物为主要能量底物，HFD组则以脂肪为主，而碳水代谢率降低后升高，最终两组底物代谢率于第5~6周时达到相似水平。HFDT组以脂肪为主要代谢底物，CONT组以碳水化合物为主要代谢底物，在训练6周后仍存在统计学差异(

0.05)。高脂饮食升高了HFD组大鼠血清胆固醇、TG、HDL-C等指标水平，但经历耐力训练的高脂饮食大鼠(HFDT组)血脂水平有所降低，且HFDT组β羟基丁酸(βHB)水平升高(

0.05)。等能量不同饮食对肝肾功能及肌肉损伤指标的影响没有明显差别。

结论

等能量高脂饮食可通过提高大鼠脂肪利用的能力，从而延长耐力运动时间，而不改变体成分或影响肝肾功能。

Abstract

Objective

To investigate the effects of a 45% high-fat diet (HFD) with isocaloric intake on energy metabolism and endurance exercise capacity in SD rats.

Methods

Twenty-four male SD rats were randomly divided into normal chow diet group (CON)

HFD group

normal chow diet+exercise training group (CONT)

and HFD+exercise training group (HFDT). The CON and CONT groups received normal chow diet

while the HFD and HFDT groups received a 45% high-fat diet with isocaloric intake. The HFDT and CONT groups underwent an endurance training of moderate-intensity running for 6 weeks. Body weight

fat mass

and lean mass were measured weekly. Energy expenditure and basal metabolic rate during rest and exercise states were measured using Pheno Master/Calo Treadmill system. Blood glucose

lipids

and creatine kinase levels were detected after the exhaustion test.

Results

In 6 weeks after intervention

the endurance exercise capacity was significantly enhanced in the HFDT group than the CONT group (

0.05). There were no obvious differences in body weight and body composition among the groups under isoenergetic feeding conditions. At rest

no statistical differences were observed in total energy expenditure and basal metabolic rate among the groups. However

prior to the 4th week

the CON group primarily metabolized carbohydrates while the HFD group primarily metabolized fats. But the carbohydrate metabolism was decreased and then increased

and the substrate metabolism rates eventually reached similar levels between the 2 groups on the 5th to 6th week. The HFDT group primarily metabolized fats while the CONT group primarily metabolized carbohydrates

with significant differences persisting after 6 weeks of training (

0.05). HFD led to elevated levels of serum cholesterol

triglycerides (TG)

and high-density lipoprotein cholesterol (HDL-C)

but

endurance training resulted in decreased lipid levels in the HFDT group

accompanied by an increase in β-hydroxybutyrate (βHB) level (

0.05). Isoenergetic diets had no significant differences in their effects on liver and kidney function or muscle damage indicators.

Conclusion

An isoenergetic HFD can improve fat utilization ability and extend endurance exercise time in rats without altering body composition or affecting liver and kidney function.

关键词

Keywords

references

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