Effect of a nutritional formula on enhancing endurance of heavy load exercise

Ruiliang ZHANG; Shuai CHEN; Xiangyuan DENG; Jundong ZHU; Hedong LANG; Mantian MI; Long YI

doi:10.16016/j.2097-0927.202309016

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Effect of a nutritional formula on enhancing endurance of heavy load exercise

Public Health and Preventive Medicine | 更新时间：2026-01-09

- Effect of a nutritional formula on enhancing endurance of heavy load exercise
- Journal of Army Medical University Vol. 46, Issue 9, Pages: 962-968(2024)
- 作者机构：
  
  400038 重庆，陆军军医大学(第三军医大学)军事预防医学系营养与食品安全研究中心，重庆市医学营养研究中心，营养与健康重庆市重点实验室
- 作者简介：
- 基金信息：
- DOI：10.16016/j.2097-0927.202309016
  CLC： R-332;R151.41;R153.4
- Received：04 September 2023，
  
  Revised：2023-11-20，
  
  Published：15 May 2024
- 稿件说明：
移动端阅览
Ruiliang ZHANG, Shuai CHEN, Xiangyuan DENG, et al. Effect of a nutritional formula on enhancing endurance of heavy load exercise[J]. Journal of Army Medical University, 2024, 46(9): 962-968.
DOI：

Ruiliang ZHANG, Shuai CHEN, Xiangyuan DENG, et al. Effect of a nutritional formula on enhancing endurance of heavy load exercise[J]. Journal of Army Medical University, 2024, 46(9): 962-968. DOI： 10.16016/j.2097-0927.202309016.

摘要

目的

研究一种提升大负荷运动耐力的复方营养制剂组方，并对其作用效果进行综合评估。

方法

C57BL/6J雄性小鼠60只，按随机数字表法分为对照组(CON组)、低剂量复方营养制剂组(low-dose nutritional formula，LDF组)、中剂量复方营养制剂组(medium-dose nutritional formula，MDF组)及高剂量复方营养制剂组(high-dose nutritional formula，HDF组)，每组15只。各组给予复方营养制剂干预2周，并分别在第1周和第2周进行适应性训练和大负荷运动。检测小鼠力竭运动时间、骨骼肌抗氧化指标(SOD、MDA、PC、GSH)、疲劳相关指标(血清UREA、LDH、LA)、肌糖原及血清运动损伤相关指标(ALT、AST、CK、CK-MB)水平，按照相关评价标准进行综合评估。

结果

LDF组、MDF组和HDF组较CON组跑步力竭时间显著延长(

0.05)，其中HDF组提升幅度最大(

0.05)；与CON组相比，不同剂量复方营养制剂组小鼠骨骼肌SOD、GSH活性显著上升(

0.05)，骨骼肌MDA、PC水平显著下降(

0.05)；骨骼PAS染色提示，MDF组、HDF组较CON组糖原含量显著提升(

0.05)，其中HDF组提升幅度最大(

0.05)；血清生化指标提示，与CON相比，不同剂量复方营养制剂组LDH、LA、ALT、AST、CK、CK-MB水平均显著下降(

0.05)。

结论

该复方营养制剂干预可以明显提升小鼠大负荷运动耐力和骨骼肌抗氧化能力，并具有抗疲劳和损伤保护作用，可推荐用于大负荷耐力训练体能保障。

Abstract

Objective

To develop a nutritional formula on enhancing the endurance of heavy load exercise

and evaluate its efficacy comprehensively.

Methods

Sixty C57BL/6J male mice were randomly divided into control group (CON group) and low-

medium- and high-dose nutritional formula groups (LDF

MDF and HDF groups)

with 15 mice in each group. Each group received intervention with nutritional formula at corresponding dose for 2 weeks

and underwent adaptive training and heavy load exercise in the 1st and 2nd weeks

respectively. Exhaustion exercise time

skeletal muscle antioxidant indicators (SOD

MDA

PC and GSH)

fatigue related indicators (serum URA

LDH and LA)

muscle glycogen

and serum exercise injury related indicators (ALT

AST

CK and CK-MB) were measured and detected in the mice

and comprehensive evaluation was conducted according to relevant evaluation standards.

Results

The LDF group

MDF group and HDF group had significantly prolonged running exhaustion time than the CON group (

0.05)

with the HDF group showing the greatest improvement (

0.05). Compared with the CON group

the activities of SOD and GSH in the skeletal muscles were significantly increased (

0.05)

while the levels of MDA and PC in skeletal muscles were obviously decreased in the 3 doses of nutritional formula groups (

0.05). PAS staining of the skeletal muscles displayed that the glycogen content was significantly increased in the MDF group and the HDF group than the CON group (

0.05)

and the highest increase was observed in the HDF group (

0.05). Biochemical test revealed that the levels of LDH

ALT

AST

and CK-MB were remarkably lower in the 3 doses of nutritional formula groups than the CON group (

0.05).

Conclusion

The nutritional formula can significantly improve the endurance and skeletal muscle antioxidant capacity in mice under heavy load exercise

and has anti-fatigue and -injury protection effects. This nutritional formula can be used to support physical fitness during heavy load endurance exercise.

关键词

Keywords

references

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