Characteristics of blood microbiota in high altitude polycythemia patients by 16S rRNA gene sequencing

Pei HUANG; He HUANG; Shenwei XIE; Yu WU; Zhifeng ZHONG; Huaping DONG; Simin ZHOU; Peng LI; Jiaxin XIE

doi:10.16016/j.2097-0927.202309007

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Characteristics of blood microbiota in high altitude polycythemia patients by 16S rRNA gene sequencing

Military Medicine | 更新时间：2026-01-09

- Characteristics of blood microbiota in high altitude polycythemia patients by 16S rRNA gene sequencing
- Journal of Army Medical University Vol. 46, Issue 10, Pages: 1075-1082(2024)
- 作者机构：
  
  1.400038 重庆，陆军军医大学(第三军医大学)高原军事医学系高原作业医学教研室
  2.730000 兰州，联勤保障部队第九四〇医院神经内科
  3.857000 西藏日喀则，陆军第九五三医院健康管理科
- 作者简介：
- 基金信息：
  
  the Natural Science Foundation of Gansu Province(22JR11RA008);the Research Project of No. 940 Hospital of Joint Logistics Support Force of PLA(2022yxky005);the Scientific Research Project of Healthcare Industry of Gansu Province(GSWSKY2022-47)
- DOI：10.16016/j.2097-0927.202309007
  CLC： R195.4;R446.119;R555.1
- Received：01 September 2023，
  
  Revised：2023-12-28，
  
  Published：30 May 2024
- 稿件说明：
移动端阅览
Pei HUANG, He HUANG, Shenwei XIE, et al. Characteristics of blood microbiota in high altitude polycythemia patients by 16S rRNA gene sequencing[J]. Journal of Army Medical University, 2024, 46(10): 1075-1082.
DOI：

Pei HUANG, He HUANG, Shenwei XIE, et al. Characteristics of blood microbiota in high altitude polycythemia patients by 16S rRNA gene sequencing[J]. Journal of Army Medical University, 2024, 46(10): 1075-1082. DOI： 10.16016/j.2097-0927.202309007.

摘要

目的

探讨高原红细胞增多症(high altitude polycythemia，HAPC)患者血液菌群变化特征及其与HAPC发病风险的相关性。

方法

采用横断面研究设计方案，以2021年在西藏日喀则陆军第九五三医院健康管理科参加体检的41名HAPC患者(HAPC组)和41名高原健康人员(对照组)为研究对象。通过高通量测序技术对血液细菌16 S核糖体RNA(16S rRNA)基因的V3-V4可变区进行检测，比较分析HAPC组和对照组血液菌群组成情况和差异。

结果

2组人员均为汉族男性，年龄、体质量指数及高原移居时间等基线数据比较差异无统计学意义(

＞0.05)。菌群多样性分析显示，HAPC组中反映血液菌群α-多样性的Simpson指数[(0.931±0.005)

(0.907±0.008)，

＜0.05

]

、Goods Coverage指数[(0.998±0.001)

(0.997±0.001)，

＜0.001)

]

和Pielou指数[(

0.597±0.011)

(0.567±0.009)，

＜0.05)

]

显著高于对照组。2组间β多样性差异有统计学意义(

0.01)。菌群相对丰度分析显示，HAPC组血液嗜糖假单胞菌属(

Pelomonas

)[(0.046±0.004)

(0.033±0.003)，

＜0.05)

]

、固氮螺菌属(

Azospirillum

)[(0.046±0.006)

(0.021±0.003)，

＜0.01)

]

、食酸菌属(

Acidovorax

)[(0.032±0.003)

(0.019±0.002)，

＜0.05)

]

的相对丰度均显著高于对照组。特征性细菌LEfSe分析显示，HAPC组标志菌群是α-变形菌纲，对照组标志菌群是毛螺菌科。

结论

HAPC患者较高原健康人群的血液菌群在多样性、相对丰度及特征性菌类方面发生显著变化，这类变化可能与HAPC的发生发展密切关联。

Abstract

Objective

To investigate the changes in blood microbiota in patients with high altitude polycythemia (HAPC) and the correlation with the risk of HAPC.

Methods

A cross-sectional trial was carried out among 41 HAPC patients (HAPC group) and 41 healthy plateau individuals (control group) who took physical examination in the Health Management Department of No. 953 Hospital of PLA Army in 2021. High-throughput sequencing technology was used to detect the V3-V4 variable region of the 16S ribosomal RNA (16S rRNA) gene in the blood smaples

and the composition and difference of the blood microbiota were compared and analyzed between the 2 groups.

Results

All the participants were male and Han people

and there were no significant differences in baseline data such as age

body mass index and plateau migration time between the 2 groups (

0.05). The α-diversity of blood microbiota in the HAPC group

including the Simpson index (0.931±0.005

0.907±0.008

0.05)

Goods Coverage index (0.998±0.001

0.997±0.001

0.001) and Pielou index (0.597±0.011

0.567±0.009

0.05) were significantly higher than th

ose in the control group. Meanwhile

obvious difference was observed in the β diversity between the 2 groups (

0.01). The relative abundance analysis of bacteria showed that

Pelomonas

(0.046±0.004

0.033±0.003

0.05)

Azospirillum

(0.046±0.006

0.021±0.003

0.01)

Acidovorax

(0.032±0.003

0.019±0.002

Azospirillum

(0.046±0.006

0.021±0.003

0.01) and

Acidovorax

(0.032±0.003

0.019±0.002

0.05) were statistically higher in the HAPC group than the control group. LEfSe analysis showed that the characteristic blood microbiota of the HAPC group were α-

Proteobacteria

and those of control group were

Trichospiridae

Conclusion

Significant changes are found in diversity

relative abundance and characteristic bacteria of the blood microbiota between the HAPC patients and healthy people at the high altitude

which might be closely associated with the occurrence and development of HAPC.

关键词

Keywords

references

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