Expression of long non-coding RNA SFTA1P and its effect on biological functions in lung squamous cell carcinoma

Weiping WAN; Weijia XIE; Tingting XIA; Ying XIANG; Na WU; Chengying LI; Yifan SHAN; Li BAI; Yafei LI

doi:10.16016/j.2097-0927.202309005

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Expression of long non-coding RNA SFTA1P and its effect on biological functions in lung squamous cell carcinoma

Basic Medicine | 更新时间：2026-01-09

- Expression of long non-coding RNA SFTA1P and its effect on biological functions in lung squamous cell carcinoma
- Journal of Army Medical University Vol. 46, Issue 11, Pages: 1226-1234(2024)
- 作者机构：
  
  1.400038 重庆，陆军军医大学(第三军医大学)军事预防医学系军队流行病学教研室
  2.400037 重庆，陆军军医大学(第三军医大学)第二附属医院呼吸内科研究所，全军呼吸病研究重点实验室
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81871896)
- DOI：10.16016/j.2097-0927.202309005
  CLC： R394.3;R730.23;R734.2
- Received：01 September 2023，
  
  Revised：2023-12-28，
  
  Published：15 June 2024
- 稿件说明：
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Weiping WAN, Weijia XIE, Tingting XIA, et al. Expression of long non-coding RNA SFTA1P and its effect on biological functions in lung squamous cell carcinoma[J]. Journal of Army Medical University, 2024, 46(11): 1226-1234.
DOI：

Weiping WAN, Weijia XIE, Tingting XIA, et al. Expression of long non-coding RNA SFTA1P and its effect on biological functions in lung squamous cell carcinoma[J]. Journal of Army Medical University, 2024, 46(11): 1226-1234. DOI： 10.16016/j.2097-0927.202309005.

摘要

目的

探究长链非编码RNA表面活性剂相关1假基因(surfactant associated 1 pseudogene，SFTA1P)在肺鳞状细胞癌(简称肺鳞癌)中的表达水平及其对肺鳞癌细胞生物学功能的影响。

方法

提取癌症基因组图谱(the cancer genome atlas

TCGA)数据库数据，分析SFTA1P在肺鳞癌组织及正常肺组织中的表达差异；qRT-PCR检测SFTA1P在人正常肺上皮细胞系(BEAS-2B)和肺鳞癌细胞系(H520和SK-MES-1)中的表达；通过转染SFTA1P过表达质粒及SFTA1P小干扰RNA分别构建SFTA1P过表达及干扰细胞模型；通过CCK-8及Transwell实验检测SFTA1P对肺鳞癌细胞生物学功能的影响；通过差异分析、相关性分析和功能富集分析探讨SFTA1P影响肺鳞癌细胞生物学功能的潜在机制。

结果

TCGA数据库肺鳞癌样本分析结果显示，SFTA1P在肺鳞癌组织中表达较正常肺组织低(

0.05)；SFTA1P在肺鳞癌细胞中表达也较人正常肺上皮细胞低(

0.05)；在肺鳞癌细胞系中，相比于H520细胞株，SFTA1P在SK-MES-1细胞株中相对低表达(

0.05)；细胞功能实验结果发现，过表达SFTA1P可抑制肺鳞癌细胞增殖、迁移及侵袭(

0.05)，敲低SFTA1P可增强肺鳞癌细胞增殖、迁移及侵袭(

0.05)；差异分析、相关性分析和功能富集分析结果表明，SFTA1P在肺鳞癌中可能抑制MYC、G2m检查点和E2f等信号通路。

结论

SFTA1P在肺鳞癌中具有抑癌功能，其可能通过抑制MYC、G2m检查点和E2f等信号通路影响肺鳞癌细胞的生物学功能。

Abstract

Objective

To investigate the expression of long non-coding RNA (lncRNA)

surfactant associated 1 pseudogene (SFTA1P) in lung squamous carcinoma and its effect on the biological functions of SFTA1P in lung squamous carcinoma cell lines.

Methods

Based on the cancer genome atlas (TCGA) database

the differential expression of SFTA1P in tumor and normal tissues were compared in patients diagnosed with lung squamous cell carcinoma. Then

the expression of SFTA1P was detected in human normal lung epithelial cell line BEAS-2B and lung squamous cell lines SK-MES-1 and H520 with real-time quantitative polymerase chain reaction (RT-qPCR). SK-MES-1 and H520 cells with overexpression and/or knockdown of SFTA1P were constructed by transfecting the overexpression plasmids (pcDNA3.1-SFTA1P) and small interfering RNAs (si-SFTA1P-1 and si-SFTA1P-2). CCK-8 assay and Transwell assay were used to investigate the effect of SFTA1P on biological functions in lung squamous carcinoma cells. Differential gene expression analysis

correlation analysis and functional enrichment analysis were employed to explore the potential mechanism that SFTA1P may affect biological functions of lung squamous cells.

Results

Analysis of TCGA showed that the expression of SFTA1P was significantly lower in lung squamous cell carcinoma tissue than adjacent normal tissue (

0.05). RT-PCR results showed that the expression of SFTA1P was obviously lower in lung squamous carcinoma cells than the human normal lung epithelial cells (

0.05). And the expression level of SFTA1P was relatively lower in the SK-MES-1 cells than the H520 cells (

0.05). Overexpression of SFTA1P suppressed the proliferation

migration and invasion of lung squamous carcinoma cells (

0.05)

while its knockdown promoted these abilities (

0.05). Differential gene expression analysis

correlation analysis and functional enrichment analysis indicated that SFTA1P may inhibit MYC

G2m checkpoints and E2f signaling pathways in lung squa

mous cell carcinoma.

Conclusion

SFTA1P shows anti-cancer function in lung squamous cell carcinoma

and it may affect the biological functions of lung squamous cell carcinoma cells through down-regulating MYC

G2m checkpoints and E2f signaling pathways.

关键词

Keywords

references

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