Effects of treadmill training on remyelination in hippocampus and cognitive function in rats exposed to acute plateau hypoxia

Qing ZHANG; Yuan LIU; Haodong LUO; Hong SU; Juan ZHONG; Ce YANG; Haiyan WANG; Sen LI; Ying YIN; Botao TAN

doi:10.16016/j.2097-0927.202307080

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Effects of treadmill training on remyelination in hippocampus and cognitive function in rats exposed to acute plateau hypoxia

Neuroscience | 更新时间：2026-01-09

- Effects of treadmill training on remyelination in hippocampus and cognitive function in rats exposed to acute plateau hypoxia
- Journal of Army Medical University Vol. 46, Issue 8, Pages: 786-795(2024)
- 作者机构：
  
  1.400010 重庆，重庆医科大学附属第二医院康复医学科
  2.400042 重庆，陆军军医大学(第三军医大学)大坪医院特殊环境战伤防治研究室，创伤与化学中毒全国重点实验室
- 作者简介：
- 基金信息：
  
  the Open Project of State Key Laboratory of Trauma, Burns and Combined Injuries(SKLKF20.2112);the National Natural Science Foundation for Young Scholars of China(82002377)
- DOI：10.16016/j.2097-0927.202307080
  CLC： R322.81;R339.4;R339.54
- Received：12 July 2023，
  
  Revised：2023-10-29，
  
  Published：30 April 2024
- 稿件说明：
移动端阅览
Qing ZHANG, Yuan LIU, Haodong LUO, et al. Effects of treadmill training on remyelination in hippocampus and cognitive function in rats exposed to acute plateau hypoxia[J]. Journal of Army Medical University, 2024, 46(8): 786-795.
DOI：

Qing ZHANG, Yuan LIU, Haodong LUO, et al. Effects of treadmill training on remyelination in hippocampus and cognitive function in rats exposed to acute plateau hypoxia[J]. Journal of Army Medical University, 2024, 46(8): 786-795. DOI： 10.16016/j.2097-0927.202307080.

摘要

目的

探讨跑台训练对急性高原低氧暴露大鼠海马髓鞘结构及认知功能的影响。

方法

SPF级6~8周龄雌性SD大鼠30只(体质量200~220 g)，6只用于损伤后髓鞘形态观察；其余24只随机分为(

=8): 对照组、低压缺氧组和跑台训练组。实验组放入海拔6 000 m的低压氧舱内，连续7 d。对照组动物放入密闭舱室内相同时间而不进行缺氧处理。跑台训练组于造模结束后第2天开始4周的跑台训练。训练结束后对各组大鼠进行旷场和Morris水迷宫实验检测观察大鼠认知功能变化；透射电镜(transmission electron microscopy，TEM)观察海马脱髓鞘改变；免疫荧光染色及Western blot检测海马CA1和CA3区少突胶质细胞转录因子2(oligodendrocyte transcription factor 2，Olig2)、髓鞘碱性蛋白(myelin basic protein，MBP)的表达情况。

结果

行为学检测结果显示: 低压缺氧组进入旷场中心区域次数、总路程和路程比及Morris水迷宫穿越平台次数和目标区域路程较对照组减少(

＜0.05)，而跑台训练组较低压缺氧组有所增加(

＜0.05)。免疫荧光染色显示: 低压缺氧组海马CA1和CA3区单位面积内Olig2阳性细胞数量及MBP的平均荧光强度较对照组明显减少(

＜0.05)，而跑台训练组较低压缺氧组有所增加(

＜0.05)。Western Blot结果显示: 低压缺氧组海马Olig2和MBP蛋白表达水平较对

照组显著降低(

＜0.01)，而跑台训练组较低压缺氧组有所增加(

＜0.01)。

结论

跑台训练能够促进急性高原低氧暴露大鼠CA1和CA3区少突胶质谱系细胞数量和髓鞘相关蛋白的表达增加，并促进海马髓鞘结构修复，从而改善缺氧导致的焦虑样行为及记忆功能障碍。

Abstract

Objective

To determine the effects of treadmill training on the structure of hippocampal myelin and cognitive function in rats exposed to acute plateau hypoxia.

Methods

With 30 SPF-grade female SD rats (aged 6-8 weeks

weighing 200-220 g)

6 of them were used for observation of myelin structure after injury

and the remaining 24 rats were randomly divided into control group

hypobaric hypoxia group and treadmill training group (

=8). The rats in above experimental groups were placed in a low-pressure oxygen chamber at an altitude of 6 000 m for 7 consecutive days

and the rats of the control group were placed in the confined chamber for the same period without hypoxia. Then

the rats of the treadmill training group received a 4-week treadmill training scheme since the day after hypoxia. Finally

all the rats were tested for cognitive function with open field test (OFT) and Morris water maze (MWM). Transmission electron microscopy (TEM) was used to observe the changes of demyelination in the hippocampus. The expression of oligodendrocyte transcription factor 2 (Olig2) and myelin basic protein (MBP) in the hippocampal CA1 and CA3 regions was measured by immunofluorescence staining and Western blotting.

Results

Behavioral tests showed that the number into the central area

total distance

distance ratio in OFT and the number of platform crossings and distance to the target area in MWM were reduced in the hypobaric hypoxia group than the control group (

0.05)

while these indexes were increased in the treadmill training group than in the hypobaric hypoxia group (

0.05). Immunofluorescence stain

ing indicated that the number of Olig2 positive cells per unit area and the mean fluorescence intensity of MBP in the CA1 and CA3 regions were significantly lessen in the hypobaric hypoxia group than the control group (

0.05)

while these indicators were higher in the treadmill training group than the hypobaric hypoxia group (

0.05). Western blotting displayed that the expression levels of Olig2 and MBP in the hippocampus were obviously lower in the hypobaric hypoxia group than the control group (

0.01)

while the levels were increased in the treadmill training group than the hypobaric hypoxia group (

0.01).

Conclusion

Treadmill training promotes the number of the oligodendrocyte spectrum cells in CA1 and CA3 regions

enhances the expression of myelin-related proteins and improves myelin repair in hippocampus of hypobaric hypoxia rats

and thereby ameliorates hypoxia-induced anxiety-like behaviors and memory dysfunction.

关键词

Keywords

references

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