Comparison on myocardial injury in rats with classic versus exertional heat stroke

Boyi ZHANG; Xue LUO; Zhen LUO; Zeze WANG; Yulong TAN; Genlin HE; Ping LI; Tingting SHEN; Xiaoqian LIU; Xuesen YANG

doi:10.16016/j.2097-0927.202306123

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Comparison on myocardial injury in rats with classic versus exertional heat stroke

Military Medicine | 更新时间：2026-01-09

- Comparison on myocardial injury in rats with classic versus exertional heat stroke
- Journal of Army Medical University Vol. 45, Issue 17, Pages: 1779-1789(2023)
- 作者机构：
  
  1.400038 重庆，陆军军医大学(第三军医大学)：军事预防医学系热带医学教研室
  2.400038 重庆，陆军军医大学(第三军医大学)：极端环境医学教育部重点实验室
- 作者简介：
- 基金信息：
- DOI：10.16016/j.2097-0927.202306123
  CLC： R322.11;R361;R594.1
- Received：30 June 2023，
  
  Revised：2023-07-18，
  
  Published：15 September 2023
- 稿件说明：
移动端阅览
Boyi ZHANG, Xue LUO, Zhen LUO, et al. Comparison on myocardial injury in rats with classic versus exertional heat stroke[J]. Journal of Army Medical University, 2023, 45(17): 1779-1789.
DOI：

Boyi ZHANG, Xue LUO, Zhen LUO, et al. Comparison on myocardial injury in rats with classic versus exertional heat stroke[J]. Journal of Army Medical University, 2023, 45(17): 1779-1789. DOI： 10.16016/j.2097-0927.202306123.

摘要

目的

比较劳力型热射病(exertional heat stroke

EHS)与经典型热射病(classical heat stroke

CHS)大鼠动物模型心肌损伤的差异，为热射病心肌损伤的临床治疗提供可能的实验室依据。

方法

80只SD大鼠按随机数表法随机分为对照组(

=10)、运动对照组(EXC，

=10)、经典热射病组(CHS，

=30)和劳力型热射病组(EHS，

=30)。对照组和EXC组大鼠置于温度(25±0.5)℃，相对湿度(50±5)%环境中；EHS组和CHS组大鼠置于模拟热气候动物

舱内，舱内环境温度(39±0.5)℃，相对湿度(50±5)%；EHS组和EXC组大鼠给予跑台运动处理，跑步速度设置为12 m/min，坡度为0°，每运动8 min休息2 min，以监测大鼠直肠温度等。核心体温达到42.3 ℃或出现瘫倒(掐尾反射消失)作为热射病的开始，之后将大鼠移至常温环境复温。采用超声心动图仪检测大鼠心功能指标，生化法检测血清心肌酶(AST、LDH、CK-MB、CK)水平，苏木精-伊红(HE)染色法检测大鼠心肌组织病理，TUNEL染色检测大鼠心肌细胞凋亡情况。采用蛋白免疫印迹法检测大鼠心肌组织中醌氧化还原酶1(Nrf2)、切割半胱天冬氨酸蛋白酶(Cl-Caspase-3)、抗凋亡蛋白(Bcl-2)、促凋亡蛋白(Bax)、血红素加氧酶-1(HO-1)的蛋白表达水平。

结果

与CHS组大鼠比较，EHS组大鼠热射病潜伏期显著缩短(

0.01)，且诱发热射病所需的热负荷显著减少(

0.01)。CHS组大鼠的脱水率显著大于EHS组(

0.01)，但EHS组大鼠的脱水速率显著高于CHS组(

0.01)。两组大鼠热射病发生时的直肠温度无显著差异(

0.05)。超声心动图提示，EHS组大鼠的左室射血分数(left ventricular ejection fraction

LVEF)显著低于CHS组大鼠(

0.05)。EHS组大鼠的AST和LDH水平显著高于CHS组大鼠(

0.01)。两组大鼠均出现急性心肌损伤的病理改变，表现为心肌细胞水肿、肌纤维排列混乱、断裂，可见炎性浸润，且EHS组心肌损伤病理评分高于CHS组(

0.05)，EHS组心肌细胞凋亡比率亦显著高于CHS组(

0.05)。氧化应激相关信号通路中，EHS组大鼠心肌组织的蛋白质表达水平显著高于CHS组。

结论

高温同时复合运动有更高的热射病易感性，EHS介导的心功能异常及心肌细胞凋亡较CHS更重，且可能与氧化应激相关信号通路活化相关。

Abstract

Objective

To compare the differences in myocardial injury in rat models of exertional heat stroke (EHS) and classic heat stroke (CHS) in order to provide possible laboratory basis for clinical treatment of heat stroke.

Methods

A total of 80 SD rats were randomly divided into control group (

=10)

exercise group (EXC

=10)

classic heat stroke group (CHS

=30) and exertional heat stroke group (EHS

=30). The rats in the control and EXC groups were exposed to a condition of ambient temperature of (25±0.5) ℃ and relative humidity of (50±5)%

and those of the EHS and CHS groups were placed in a simulated hot climate chamber with an ambient temperature of (39.0±0.5) ℃ and a relative humidity of (50±5)%. The rats in the EHS and EXC groups were admini

stered to treadmill exercise treatment under a running speed of 12 m/min and a slope of 0°

and rested for 2 min in every 8 min of exercise to monitor rectal temperature. When the core body temperature reached 42.3 ℃ or collapsed (tail pinching reflex disappeared)

the rats were taken to normal temperature and rewarmed. Echocardiography was used to detect the cardiac function

and serum myocardial enzymes (AST

LDH

CK-MB and CK) were detected by biochemical analyzer. HE staining was used to detect the pathological changes of rat myocardial tissue. The apoptosis of cardiomyocytes was detected by TUNEL staining. The expression levels of quinone oxidoreductase 1 (Nrf2)

cleaved Caspase-3

anti-apoptotic protein (Bcl-2)

pro-apoptotic protein (Bax) and heme oxygenase-1 (HO-1) were detected by Western blotting.

Results

Compared with the CHS group

the EHS group had significantly shortened latent period (

0.01) and reduced heat load in heat stroke (

0.01). The CHS group obtained obviously higher dehydration rate (

0.01) but lower dehydration velocity (

0.01) than the CHS group. There was no significant difference in rectal temperature between the 2 heat stroke groups (

0.05). Echocardiography indicated that statistically lower left ventricular ejection fraction (LVEF) (

0.05) and higher AST and LDH levels (

0.01) were observed in the EHS group than the CHS group. The pathological changes of acute myocardial injury including myocardial cell edema

disordered and broken muscle fibers

and inflammatory infiltration

were observed in both groups

but the pathological score of myocardial injury was notably higher in the EHS group than the CHS group (

0.05)

so was the apoptotic rate of cardiomyocytes (

0.05). The protein levels of oxidative stress-related signaling pathway-related proteins w

ere significantly higher in the EHS group than the CHS group.

Conclusion

High ambient temperature combined with exercise has a higher susceptibility to heat stroke. EHS-mediated abnormal cardiac dysfunction and myocardial apoptosis were more severe than CHS inducement

which may be related to the activation of oxidative stress-related signaling pathways.

关键词

Keywords

references

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