FLI-1 ameliorates cardiac hypertrophy by inhibiting IGF-1R/Gi/PLC pathway <italic style="font-style: italic">via</italic> up-regulation of Klotho

Gang TANG; Weiwei WANG; Li ZHONG; Jun LONG; Liangyi SI

doi:10.16016/j.2097-0927.202305112

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FLI-1 ameliorates cardiac hypertrophy by inhibiting IGF-1R/Gi/PLC pathway via up-regulation of Klotho

Basic Medicine | 更新时间：2026-01-09

- FLI-1 ameliorates cardiac hypertrophy by inhibiting IGF-1R/Gi/PLC pathway via up-regulation of Klotho
- Journal of Army Medical University Vol. 45, Issue 21, Pages: 2231-2238(2023)
- 作者机构：
  
  401120 重庆，重庆医科大学附属第三医院心血管内科
- 作者简介：
- 基金信息：
  
  the General Program of Natural Science Foundation of Chongqing(cstc2021jcyj-msxmX0307)
- DOI：10.16016/j.2097-0927.202305112
  CLC： R329.26;R363.22;R542.2
- Received：30 May 2023，
  
  Revised：2023-06-22，
  
  Published：15 November 2023
- 稿件说明：
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Gang TANG, Weiwei WANG, Li ZHONG, et al. FLI-1 ameliorates cardiac hypertrophy by inhibiting IGF-1R/Gi/PLC pathway via up-regulation of Klotho[J]. Journal of Army Medical University, 2023, 45(21): 2231-2238.
DOI：

Gang TANG, Weiwei WANG, Li ZHONG, et al. FLI-1 ameliorates cardiac hypertrophy by inhibiting IGF-1R/Gi/PLC pathway via up-regulation of Klotho[J]. Journal of Army Medical University, 2023, 45(21): 2231-2238. DOI： 10.16016/j.2097-0927.202305112.

摘要

目的

探讨转录因子FLI-1通过Klotho介导的IGF-1R/Gi/PLC途径对心肌细胞肥大的影响。

方法

将大鼠心肌细胞(H9c2)分为对照组、模型组、模型+NC Vector组、模型+pcDNA-FLI-1组、模型+NC Vector+NC siRNA组、模型+NC Vector+Klotho siRNA组、模型+pcDNA-FLI-1+NC siRNA组和模型+pcDNA-FLI-1+Klotho siRNA组等共8组。通过用异丙肾上腺素(isoproterenol，ISO)处理细胞，建立心肌细胞肥大模型，细胞分别转染pcDNA-FLI-1和Klotho siRNA。采用流式细胞术观测细胞凋亡，鬼笔环肽染色观测细胞肥大面积；采用Western blot检测细胞凋亡、肥大及IGF-1R/Gi/PLC途径相关蛋白的表达。

结果

与对照组比较，模型组细胞凋亡率、心肌细胞表面积及IGF-1R、GNAI1和PLCG1表达显著增加(

0.05)，表明建模成功；与模型+NC Vector组比较，模型+pcDNA-FLI-1组细胞凋亡、心肌细胞表面积及IGF-1R、GNAI1和PLCG1表达显著降低(

0.05)。与模型+NC Vector+NC siRNA组相比，模型+NC Vector+Klotho siRNA组细胞凋亡、心肌细胞表面积及IGF-1R、GNAI1和PLCG1表达显著增加(

0.05)；与模型+pcDNA-FLI-1+NC siRNA组相比，模型+pcDNA-FLI-1+Klotho siRNA组细胞凋亡、心肌细胞表面积及IGF-1R、GNAI1和PLCG1表达显著降低(

0.05)。

结论

FLI-1能够通过上调Klotho抑制IGF-1R/Gi/PLC途径，减轻心肌细胞凋亡及心肌细胞肥大面积。

Abstract

Objective

To investigate the effect of FLI-1 on Klotho-mediated IGF-1R/Gi/PLC pathway on cardiomyocyte hypertrophy.

Methods

The cells were divided into 8 groups: control group

model group

model+NC Vector group

model+pcDNA-FLI-1 group

model+NC Vector+NC siRNA group

model+NC Vector+Klotho siRNA group

model+pcDNA-FLI-1+NC siRNA group and model+pcDNA-FLI-1+Klotho siRNA group. Cardiomyocyte hypertrophy models were established by treating cells with isoproterenol (ISO)

and the latter cells were transfected with pcDNA-FLI-1 and Klotho siRNA

respectively. Apoptosis was detected by flow cytometry; hypertrophic area was detected by phalloidin staining; apoptosis

hypertrophy and expression of IGF-1R/Gi/PLC pathway-related proteins were detected by Western blotting.

Results

Compared with the control group

the ISO group had increased apoptotic rate

cardiomyocyte surface area and IGF-1R

GNAI1 and PLCG1 expression

indicating successful modeling; compared with the model +NC Vector group

the model +pcDNA-FLI-1 group had decreased apoptosis

cardiomyocyte surface area and IGF-1R

GNAI1 and PLCG1 expression. Apoptosis

cardiomyocyte surface area

and IGF-1R

GNAI1 and PLCG1 expression were increased in the model +NC Vector+Klotho siRNA group compared with the model +NC Vector+NC siRNA group; apoptosis

cardiomyocyte surface area

and IGF-1R

GNAI1 and PLCG1 expression were decreased in the model +pcDNA-FLI-1+Klotho siRNA group compared with the model +pcDNA-FLI-1+NC siRNA group.

Conclusion

FLI-1 can inhibit IGF-1R/Gi/PLC pathway and reduce myocardial apoptosis and myocardial hypertrophy by up-regulating Klotho.

关键词

Keywords

references

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⁰

FLI-1 ameliorates cardiac hypertrophy by inhibiting IGF-1R/Gi/PLC pathway via up-regulation of Klotho

DOI：10.16016/j.2097-0927.202305112

摘要

Abstract

关键词

Keywords

references