Lycopene improves social disorder and reduces hippocampal inflammation in BTBR mice

Jianghui LI; Dabing LI; Xianyu ZHU; Mei CHEN; Xiaotang FAN; Pingsu MAO

doi:10.16016/j.2097-0927.202302024

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Lycopene improves social disorder and reduces hippocampal inflammation in BTBR mice

Neuroscience | 更新时间：2026-01-09

- Lycopene improves social disorder and reduces hippocampal inflammation in BTBR mice
- Journal of Army Medical University Vol. 45, Issue 14, Pages: 1483-1492(2023)
- 作者机构：
  
  1.646000 四川泸州，西南医科大学基础医学院：肿瘤医学研究所
  2.400038 重庆，陆军军医大学(第三军医大学) 医学心理系军事认知心理学教研室
  3.646000 四川泸州，西南医科大学基础医学院：基础医学院生理学教研室
- 作者简介：
- 基金信息：
- DOI：10.16016/j.2097-0927.202302024
  CLC： R285.5;R338.26;R749.94
- Received：06 February 2023，
  
  Revised：2023-03-01，
  
  Published：30 July 2023
- 稿件说明：
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Jianghui LI, Dabing LI, Xianyu ZHU, et al. Lycopene improves social disorder and reduces hippocampal inflammation in BTBR mice[J]. Journal of Army Medical University, 2023, 45(14): 1483-1492.
DOI：

Jianghui LI, Dabing LI, Xianyu ZHU, et al. Lycopene improves social disorder and reduces hippocampal inflammation in BTBR mice[J]. Journal of Army Medical University, 2023, 45(14): 1483-1492. DOI： 10.16016/j.2097-0927.202302024.

摘要

目的

研究番茄红素(Lycopene，Lyc)对BTBR T

Itpr3

/ J(BTBR)自闭症模型小鼠海马组织中炎症水平、社交缺陷、认知障碍和重复刻板行为的影响。

方法

7~8周龄成年雄性健康C57BL/6(C57)与BTBR小鼠随机数字表法分为4组(

=8)：C57+Veh组、C57+Lyc组、BTBR+Veh组、BTBR+Lyc组。C57+Lyc组和BTBR+Lyc组小鼠予以番茄红素(20 mg/kg)灌胃，1次/d，共7 d；C57+Veh组小鼠和BTBR+Veh组小鼠给予等体积的玉米油。给药7 d后开始三箱社交、雌雄交互、理毛、埋珠、开场实验、新物体识别、筑巢和Y迷宫等行为实验。结束后立即收取脑组织样本，进行RT-qPCR、Western blot实验。

结果

行为学结果显示：三箱社交实验中，BTBR+Veh组小鼠在陌生物体所在的箱体时间更多，表现出对陌生物体更偏好(

0.05)；而Lyc治疗后小鼠表现出对陌生小鼠更偏好(

0.05)。理毛、埋珠实验中，BTBR+Veh组小鼠较C57+Veh组小鼠的重复刻板行为显著增多(

0.05)，Lyc治疗后显著减少了BTBR小鼠的重复刻板行为(

0.05)。BTBR+Veh组小鼠较C57+Veh组小鼠海马组织中NLRP3炎症小体在mRNA和蛋白水平均显著增高(

0.05)；白细胞介素-1β(interleukin-1β，IL-1β)、IL-18、IL-6、核因子κB (nuclear factor kappaB，NF-κB)、肿瘤坏死因子

(tumor necrosis factor-

，TNF-

) 在mRNA水平显著增高(

0.05)；Lyc治疗后BTBR小鼠各炎症因子均显著降低(

0.05)。

结论

番茄红素治疗通过降低BTBR小鼠海马中NLRP3炎症小体的水平，改善BTBR小鼠三箱社交缺陷和重复刻板行为。

Abstract

Objective

To investigate the effects of lycopene (Lyc) on hippocampal inflammation

social deficits and repetitive stereotyped behaviors in BTBR T

Itpr3tf/J autistic mice (BTBR

a mouse strain showing lower sociability as a model for autism spectrum disorder).

Methods

Adult male C57BL/6J (C57) and BTBR mice aged 7-8 weeks were randomly divided into (

=8): C57+Veh group

C57+Lyc group

BTBR+Veh group and BTBR+Lyc group. The mice from the C57+Lyc group and BTBR+Lyc group were given 20 mg/kg Lyc by gavage once a day for 7 d

and those of the C57+Veh group and BTBR+Veh mice were given equal volume of corn oil. Then three-chamber social test

male-female reciprocal social interactions

self-grooming

marble buying

open field test

object recognition test

nest building

and Y-maze test were conducted in these mice. Immediately after the behavioral tests

the

mice were sacrificed and their brain samples were harvested for RT-qPCR and Western blotting.

Results

Behavioral results showed that in the three-chamber social test

the mice of the BTBR+Veh group spent longer time in the chamber with the object

showing much curiosity about the object (

0.05)

while the mice after Lyc treatment showed a preference for strange mice (

0.05). In the self-grooming and marble burying behavior test

the BTBR+Veh group showed markedly increased repeated stereotyped behaviors than the C57+Veh group mice (

0.05)

which could be alleviated by Lyc treatment (

0.05). The hippocampal expression of NLRP3 at mRNA and protein levels (

0.05)

and the mRNA levels of IL-1β

IL-18

IL-6

NF-κB

and TNF-

were significantly increased in the BTBR+Veh group mice when compared with the C57+Veh group mice

and Lyc treatment obviously reversed above changes (

0.05).

Conclusion

Lyc treatment improves three-chamber social deficits and repetitive stereotyped behaviors in BTBR mice by reducing NLRP3 inflammasome in the hippocampus of BTBR mice.

关键词

Keywords

references

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