Correlation between sperm DNA methylation level and semen quality among 73 college students in Chongqing, China
- Journal of Army Medical University Vol. 45, Issue 9, Pages: 920-926(2023)
- 作者机构：
1.610041 成都，四川大学华西公共卫生学院
2.400038 重庆，陆军军医大学(第三军医大学)军事预防医学系毒理学研究所
- 作者简介：
- 基金信息：the National Natural Science Foundation for Young Scholars of China(82003429)
DOI：10.16016/j.2097-0927.202212134
CLC： R195.1;R321.1;R394.1Received：21 December 2022，
Revised：2023-02-25，
Published：15 May 2023
- 稿件说明：
移动端阅览
Lihong WANG, Shijun HE, Qing CHEN, et al. Correlation between sperm DNA methylation level and semen quality among 73 college students in Chongqing, China[J]. Journal of Army Medical University, 2023, 45(9): 920-926.
Lihong WANG, Shijun HE, Qing CHEN, et al. Correlation between sperm DNA methylation level and semen quality among 73 college students in Chongqing, China[J]. Journal of Army Medical University, 2023, 45(9): 920-926. DOI： 10.16016/j.2097-0927.202212134.
摘要
目的
2
探讨人精子DNA甲基化水平与精液常规参数和精子染色质结构完整性的关系。
方法
2
纳入2014年重庆市大学生男性生殖健康(male reproductive health in Chongqing college students，MARHCS)队列的73名志愿者，分别采用计算机辅助精子分析系统(computer-aided sperm analysis，CASA)和精子染色质结构分析(sperm chromatin structure assay，SCSA)系统检测分析精液常规参数和精子染色质结构完整性指标。通过酶联免疫吸附测定法(ELISA)检测精子DNA 5mC含量，反映精子DNA甲基化水平。同时采用调查问卷收集志愿者的年龄、BMI、禁欲时间及生活方式等数据。多元线性回归模型分析精子DNA 5mC含量与精液参数和精子染色质结构完整性的相关性，同时比较高水平和低水平精子DNA 5mC组(按照5mC%中位数二分类)志愿者各级精子百分率的差异。
结果
2
志愿者各项精液常规参数(中位数)：精液体积为4.18 mL，精子密度为55.60×10
6
/mL，精子总数为246.93×10
6
，精子活力为57.00%；染色质结构完整性：精子DNA碎片化指数(DNA fragmentation index，DFI)和高DNA着色性(high DNA stainability，HDS)指标的中位数分别是10.99%和3.39%；精子DNA 5mC水平的中位数为2.63%。校正混杂因素后，多元线性回归结果显示精子DNA 5mC与精子密度(
β
= -0.22，95%
CI
：-0.36~-0.06，
P
=0.008)和精子总数(
β
= -0.20，95%
CI
：-0.33~-0.05，
P
=0.015)负相关，与精子DFI和HDS指标的相关性分析无统计学意义(
P
>
0.05)。与低水平精子DNA 5mC组相比，高水平精子DNA 5mC组各级精子百分率差异无统计学意义(
P
>
0.05)。
结论
2
精子DNA甲基化水平升高可能是精子密度和精子总数下降的标志，而与精子染色质结构完整性指标无关。
Abstract
Objective
2
To investigate the association of sperm DNA methylation level with routine semen parameters and sperm chromatin integrity.
Methods
2
A total of 73 male individuals who were sampled from our previously established cohort of the male reproductive health in Chongqing college students (MARHCS) study were recruited in this study. The routine semen parameters (semen volume
sperm density
total sperm count and sperm motility) and indicators of sperm chromatin integrity were detected and analyzed with computer-aided sperm analysis system (CASA) and sperm chromatin structure analysis (SCSA)
respectively. ELISA was used to detect the sperm DNA 5mC content
which reflected the methylation level of sperm DNA. Meanwhile
age
BMI
abstinence time and lifestyle of the volunteers were collected through questionnaire survey. Multiple linear regression model was applied to explore the correlation of sperm DNA 5mC content with routine semen parameters and sperm chromatin integrity. The differences in percentages of different grade of sperm were compared in the high and low 5mC% groups (binary classification according to the median value of 5mC%).
Results
2
The semen routine parameters (median value) of the volunteers were as follows: semen volume was 4.18 mL
sperm density was 55.60×10
6
/mL
total sperm count was 246.93×10
6
and sperm motility was 57.00%; Sperm chromatin was in structural integrity
with a median value of sperm DNA fragmentation index (DFI) and high DNA stainablity (HDS) of 10.99% and 3.39%
respectively. The median value of sperm DNA 5mC level was 2.63%. After adjustment for confounding factors
multiple linear regression analysis showed that sperm DNA 5mC level was negatively correlated with sperm density (β=-0.22
95%
CI
: -0.36~-0.06
P
=0.008) and total sperm count (β=-0.20
95%
CI
: -0.33~-0.05
P
=0.015)
but no statistical correlations were observed in sperm DNA 5mC level with DFI and HDS (
P
>
0.05). Meanwhile
there were no significant differences in the percentages of different grade of sperm (grade A~D) in the high 5mC group than the low 5mC group (
P
>
0.05).
Conclusion
2
Elevated sperm DNA methylation level may be a biomarker for the decrease of sperm density and total sperm count
while is not correlated with indicators of sperm chromatin integrity.
关键词
Keywords
references
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