Diversity and functional characteristics of intestinal microbiome in patients with varicocele
- Journal of Army Medical University Vol. 45, Issue 6, Pages: 493-499(2023)
- 作者机构：
400037 重庆，陆军军医大学(第三军医大学)第二附属医院消化内科
- 作者简介：
- 基金信息：the Pre-research Project of Special Military Medical Funds for Scientific and Innovative Capacity Improvement of Army Medical University(2019XYY17);the Incubation Project of Youth Training Progrom of Military Medical Science and Technology(20QNPY013)
DOI：10.16016/j.2097-0927.202212019
CLC： Q939.121;R394.5;R697.24Received：02 December 2022，
Revised：2023-01-05，
Published：30 March 2023
- 稿件说明：
移动端阅览
Yisong PENG, Shuang LIU, Li TANG, et al. Diversity and functional characteristics of intestinal microbiome in patients with varicocele[J]. Journal of Army Medical University, 2023, 45(6): 493-499.
Yisong PENG, Shuang LIU, Li TANG, et al. Diversity and functional characteristics of intestinal microbiome in patients with varicocele[J]. Journal of Army Medical University, 2023, 45(6): 493-499. DOI： 10.16016/j.2097-0927.202212019.
摘要
目的
2
利用宏基因组学分析精索静脉曲张(varicocele
VC)患者和健康对照人群的肠道菌群多样性和菌群组成变化。
方法
2
选取2020年1~12月就诊于陆军军医大学第二附属医院的精索静脉曲张患者(
n
=15)和健康对照人群(
n
=15)的新鲜粪便并提取DNA，采用宏基因组学测序分析受试者的肠道菌群多样性、结构特征和功能差异。
结果
2
两组年龄、BMI差异均无统计学意义(
P
>
0.05)。患者组的肠道菌群与对照组的肠道菌群相比，Alpha多样性无显著差异(
P
>
0.05)；Beta多样性差异有统计学意义(
P
＜0.01)。两组优势菌群均为厚壁菌门(
firmicutes
)、拟杆菌门(
bacteroidetes
)、变形菌门(
proteobacteria
)、放线菌门(
actinobacteria
)、梭杆菌门(
fusobacteria
)；在种水平上，患者组的优势菌包括黄杆菌属(
flavonifractor plautii
)、甲烷短
杆菌属(
methanobrevibacter smithii
)、肠杆菌属(
enterobacter roggenkampii
)等，而对照组包括考拉杆菌属(
phascolarctobacterium faecium
)、Romboutsia属(
romboutsia ilealis
)、双歧杆菌属(
bifidobacterium bifidum
)、普雷沃氏菌属(
prevotella jejuni
)、梭菌属(
clostridium perfringens
)等。功能分析发现D-galactarate degradation I、Superpathway of D-glucarate and D-galactarate degradation、D-glucarate degradation I等多条促进D-半乳糖降解的信号通路较对照组显著上调，肠球菌属(
enterococcus faecalis
)、黄杆菌属(
flavonifractor plautii
)、拉乌尔菌属(
raoultella terrigena
)等肠道菌群与上调的信号通路相关联。
结论
2
精索静脉曲张患者与健康对照人群相比肠道菌群构成有特征性改变，数条促进D-半乳糖降解的信号通路有显著上调，肠道菌群改变可能为精索静脉曲张患者机体功能发生病理改变的机制之一。
Abstract
Objective
2
To investigate the changes of intestinal microbiome in patients with varicocele (VC) with macrogenomics.
Methods
2
Fresh feces from 15 VC patients and 15 healthy individuals were collected
and DNA sequences were extracted from the feces. The diversity and functional characteristics were analyzed by metagenome sequencing methods.
Results
2
There were no statistical differences in age and body mass index (BMI) between the 2 groups (
P
>
0.05). The Alpha diversity results showed no significant difference was observed between them (
P
>
0.05). The Beta diversity results indicated that the species diversity had significant differences in the feces between the VC patients and healthy individuals (
P
<
0.01). The dominant floras in both the VC patients and healthy individuals were mainly
Firmicutes
Bacteroidetes
Proteobacteria
Actinobacteria
and
Fusobacteria
. At species level
the dominant floras in VC patients included
Flavonifractor plautii
Methanobrevibacter smithii
Enterobacter roggenkampii
etc
and those in the healthy individuals were
Phascolarctobacterium faecium
Romboutsia ilealis
Bifidobacterium bifidum
Prevotella jejuni
Clostridium perfringens
etc
. Metagenomics data identified significant up-regulation of D-glucarate degradation pathways
including D-galactarate degradation I
superpathway of D-glucarate and D-galactarate degradation
D-glucarate degradation I
etc
. The alterations were associated with intestinal microbiomes
Enterococcus faecalis
Flavonifractor plautii
Raoultella terrigena
etc
.
Conclusion
2
There are obvious characteristics changes in microflora structure between VC patients and healthy controls. Up-regulation of D-glucarate degradation pathways are found in VC patients. These changes of intestinal microbiome might be one of the underlying pathophysiological mechanism of VC.
关键词
Keywords
references
ALSAIKHAN B, ALRABEEAH K, DELOUYA G, et al . Epidemiology of varicocele[J ] . Asian J Androl, 2016, 18(2): 179-181. DOI: 10.4103/1008-682X.172640.
艾庆燕, 赵豫凤, 王艳梅, 等. 精索静脉曲张致男性不育机制研究进展[J]. 中国全科医学, 2011, 14(24): 2813-2815. DOI: 10.3969/j.issn.1007-9572.2011.24.039.
AI Q Y, ZHAO Y F, WANG Y M, et al . Progress in the mechanism of varicocele-induced male infertility[J ] . Chin Gen Pract, 2011, 14(24): 2813-2815. DOI: 10.3969/j.issn.1007-9572.2011.24.039.
MASSON P, BRANNIGAN R E. The varicocele[J]. Urol Clin North Am, 2014, 41(1): 129-144. DOI: 10.1016/j.ucl.2013.08.001.
JENSEN C F S, OSTERGREN P, DUPREE J M, et al . Varicocele and male infertility[J ] . Nat Rev Urol, 2017, 14(9): 523-533. DOI: 10.1038/nrurol.2017.98.
HASSANIN A M, AHMED H H, KADDAH A N. A global view of the pathophysiology of varicocele[J]. Andrology, 2018, 6(5): 654-661. DOI: 10.1111/andr.12511.
LUNDY S D, SANGWAN N, PAREKH N V, et al . Functional and taxonomic dysbiosis of the gut, urine, and semen microbiomes in male infertility[J ] . Eur Urol, 2021, 79(6): 826-836. DOI: 10.1016/j.eururo.2021.01.014.
BAJIC P, WOLFE A J. The microbiome of male infertility: paving the road ahead[J]. Eur Urol, 2021, 79(6): 837-838. DOI: 10.1016/j.eururo.2021.02.009.
BÄCKHED F, LEY R E, SONNENBURG J L, et al . Host-bacterial mutualism in the human intestine[J ] . Science, 2005, 307(5717): 1915-1920. DOI: 10.1126/science.1104816.
CASH H L, WHITHAM C V, BEHRENDT C L, et al . Symbiotic bacteria direct expression of an intestinal bactericidal lectin[J ] . Science, 2006, 313(5790): 1126-1130. DOI: 10.1126/science.1127119.
TURNBAUGH P J, LEY R E, MAHOWALD M A, et al . An obesity-associated gut microbiome with increased capacity for energy harvest[J ] . Nature, 2006, 444(7122): 1027-1031. DOI: 10.1038/nature05414.
CHOW J, LEE S M, SHEN Y, et al . Host-bacterial symbiosis in health and disease[J ] . Adv Immunol, 2010, 107: 243-274. DOI: 10.1016/B978-0-12-381300-8.00008-3.
LEE K A, LUONG M K, SHAW H, et al . The gut microbiome: what the oncologist ought to know[J ] . Br J Cancer, 2021, 125(9): 1197-1209. DOI: 10.1038/s41416-021-01467-x.
RELMAN D A, LIPSITCH M. Microbiome as a tool and a target in the effort to address antimicrobial resistance[J]. Proc Natl Acad Sci USA, 2018, 115(51): 12902-12910. DOI: 10.1073/pnas.1717163115.
BUTEL M J, WALIGORA-DUPRIET A J, WYDAU-DEMATTEIS S. The developing gut microbiota and its consequences for health[J]. J Dev Orig Health Dis, 2018, 9(6): 590-597. DOI: 10.1017/S2040174418000119.
BISHEHSARI F, VOIGT R M, KESHAVARZIAN A. Circadian rhythms and the gut microbiota: from the metabolic syndrome to cancer[J]. Nat Rev Endocrinol, 2020, 16(12): 731-739. DOI: 10.1038/s41574-020-00427-4.
SAHIN Z, CELIK-OZENCI C, AKKOYUNLU G, et al . Increased expression of interleukin-1alpha and interleukin-1beta is associated with experimental varicocele[J ] . Fertil Steril, 2006, 85(Suppl 1): 1265-1275. DOI: 10.1016/j.fertnstert.2005.10.025.
ISHIKAWA T, FUJIOKA H, ISHIMURA T, et al . Increased testicular 8-hydroxy-2'-deoxyguanosine in patients with varicocele[J ] . BJU Int, 2007,100(4): 863-866. DOI: 10.1111/j.1464-410X.2007.07008.x.
LIANG M, WEN J, DONG Q, et al . Testicular hypofunction caused by activating p53 expression induced by reactive oxygen species in varicocele rats[J ] . Andrologia, 2015, 47(10): 1175-1182. DOI: 10.1111/and.12400.
ALTINTAS R, EDIZ C, CELIK H, et al . The effect of varicocoelectomy on the relationship of oxidative stress in peripheral and internal spermatic vein with semen parameters[J ] . Andrology, 2016, 4(3): 442-446. DOI: 10.1111/andr.12172.
CHO C L, ESTEVES S C, AGARWAL A. Novel insights into the pathophysiology of varicocele and its association with reactive oxygen species and sperm DNA fragmentation[J]. Asian J Androl, 2016, 18(2): 186-193. DOI: 10.4103/1008-682X.170441.
SADEGHI N, ERFANI-MAJD N, TAVALAEE M, et al . Signs of ROS-associated autophagy in testis and sperm in a rat model of varicocele[J ] . Oxid Med Cell Longev, 2020, 2020: 5140383. DOI: 10.1155/2020/5140383.
XIROPOTAMOU O, TSILI A C, ASTRAKAS L, et al . A preliminary study of the biochemical environment of infertile testes with clinical varicocele[J ] . Eur J Radiol, 2020, 127: 108989. DOI: 10.1016/j.ejrad.2020.108989.
AGHAIE A, LECHAPLAIS C, SIRVEN P, et al . New insights into the alternative D-glucarate degradation pathway[J ] . J Biol Chem, 2008, 283(23): 15638-15646. DOI: 10.1074/jbc.M800487200.
CHANG L, LIU X, LIU J, et al . D-galactose induces a mitochondrial complex I deficiency in mouse skeletal muscle: potential benefits of nutrient combination in ameliorating muscle impairment[J ] . J Med Food, 2014,17(3): 357-364. DOI: 10.1089/jmf.2013.2830.
ZHANG S C, DONG Z Q, PENG Z S, et al . Anti-aging effect of adipose-derived stem cells in a mouse model of skin aging induced by D-galactose[J ] . PLoS One, 2014, 9(5): e97573. DOI: 10.1371/journal.pone.0097573.
DU Z D, YANG Q, ZHOU T, et al . D-galactose-induced mitochondrial DNA oxidative damage in the auditory cortex of rats[J ] . Mol Med Rep, 2014, 10(6): 2861-2867. DOI: 10.3892/mmr.2014.2653.
HADZI-PETRUSHEV N, STOJKOVSKI V, MITROV D, et al . D-galactose induced changes in enzymatic antioxidant status in rats of different ages[J ] . Physiol Res, 2015, 64(1): 61-70. DOI: 10.33549/physiolres.932786.
FABER F, TRAN L, BYNDLOSS M X, et al . Host-mediated sugar oxidation promotes post-antibiotic pathogen expansion[J ] . Nature, 2016, 534(7609): 697-699. DOI: 10.1038/nature18597.
朱亚珍, 朱虹光. D-半乳糖致衰老动物模型的建立及其检测方法[J]. 复旦学报(医学版), 2007, 34(4): 617-619. DOI: 10.3969/j.issn.1672-8467.2007.04.036.
ZHU Y Z, ZHU H G. Establishment and measurement of D-galactose induced aging model[J]. Fudan Univ J Med Sci, 2007, 34(4): 617-619. DOI: 10.3969/j.issn.1672-8467.2007.04.036.
李文彬, 韦丰, 范明, 等. D-半乳糖在小鼠上诱导的拟脑老化效应[J]. 中国药理学与毒理学杂志, 1995, 9(2): 93-95.
LI W B, WEI F, FAN M, et al . Mimetic brain aging effect induced by D-galactose in mice[J ] . Chin J Pharmacol Toxicol, 1995, 9(2): 93-95.
SONG X, BAO M, LI D, et al . Advanced glycation in D-galactose induced mouse aging model[J ] . Mech Ageing Dev, 1999, 108(3): 239-251. DOI: 10.1016/s0047-6374(99)00022-6.
AZMAN K F, ZAKARIA R. D-Galactose-induced accelerated aging model: an overview[J]. Biogerontology, 2019, 20(6): 763-782. DOI: 10.1007/s10522-019-09837-y.
VAN CAUWENBERGHE O R, MAKHMOUDOVA A, MCLEAN M D, et al . Plant pyruvate-dependent gamma-aminobutyrate transaminase: identification of an arabidopsisc DNA and its expression in escherichia coli[J ] . Can J Bot, 2002, 80(9): 933-941. DOI: 10.1139/b02-087.
AGARWAL A, HAMADA A, ESTEVES S C. Insight into oxidative stress in varicocele-associated male infertility: part 1[J]. Nat Rev Urol, 2012, 9(12): 678-690. DOI: 10.1038/nrurol.2012.197.
HAMADA A, ESTEVES S C, AGARWAL A. Insight into oxidative stress in varicocele-associated male infertility: part 2[J]. Nat Rev Urol, 2013, 10(1): 26-37. DOI: 10.1038/nrurol.2012.198.
MEKHEDOV S, CAHOON E B, OHLROGGE J. An unusual seed-specific 3-ketoacyl-ACP synthase associated with the biosynthesis of petroselinic acid in coriander[J]. Plant Mol Biol, 2001, 47(4): 507-518. DOI: 10.1023/A:1011832611885.
SCHUJMAN G E, DE MENDOZA D. Transcriptional control of membrane lipid synthesis in bacteria[J]. Curr Opin Microbiol, 2005, 8(2): 149-153. DOI: 10.1016/j.mib.2005.02.003.
PENG Z, CHENG S Y, KOU Y, et al . The gut microbiome is associated with clinical response to anti-PD-1/PD-L1 immunotherapy in gastrointestinal cancer[J ] . Cancer Immunol Res, 2020, 8(10): 1251-1261. DOI: 10.1158/2326-6066.CIR-19-1014.
0
Views
0
下载量
0
CSCD
- L-PDFDownload
- Meta-XMLDownload
- BibTeXDownload
- EndnoteDownload
- RefMan Download
- NoteExpressDownload
- NoteFirstDownload
Publicity Resources
Related Articles
Related Author
Related Institution
- Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19
京公网安备11010802024621 - It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
- Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
