Combined exposure to noise and CO induces adverse male reproductive effects in rats

Yingqing LI; Chang LIU; Zhonghao ZHANG; Jiankang WANG; Lei SUN; Fei HAN; Jia CAO; Jinyi LIU

doi:10.16016/j.2097-0927.202212004

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Combined exposure to noise and CO induces adverse male reproductive effects in rats

Monographic Reports: Effect and mechanism of environmental factors on male reproductive health | 更新时间：2026-01-09

- Combined exposure to noise and CO induces adverse male reproductive effects in rats
- Journal of Army Medical University Vol. 45, Issue 9, Pages: 911-919(2023)
- 作者机构：
  
  400038 重庆，陆军军医大学(第三军医大学)军事预防医学系毒理学研究所
- 作者简介：
- 基金信息：
  
  the Project of Scientific Research for Military Family Plan(16JS008);the Key Program of National Natural Science Foundation of China(82130097)
- DOI：10.16016/j.2097-0927.202212004
  CLC： R136;R698.2;R821.2
- Received：01 December 2022，
  
  Revised：2023-02-26，
  
  Published：15 May 2023
- 稿件说明：
移动端阅览
Yingqing LI, Chang LIU, Zhonghao ZHANG, et al. Combined exposure to noise and CO induces adverse male reproductive effects in rats[J]. Journal of Army Medical University, 2023, 45(9): 911-919.
DOI：

Yingqing LI, Chang LIU, Zhonghao ZHANG, et al. Combined exposure to noise and CO induces adverse male reproductive effects in rats[J]. Journal of Army Medical University, 2023, 45(9): 911-919. DOI： 10.16016/j.2097-0927.202212004.

摘要

目的

探讨密闭舱室噪声、一氧化碳(carbon monoxide，CO)单独和联合染毒对雄性大鼠生殖系统的损伤效应，评价二者是否具有联合效应以及损伤可能涉及的信号通路。

方法

50只雄性SD大鼠，采取随机数字表法分为空白对照组、阴性对照组(单纯束缚，不染毒)、噪声染毒组(噪声强度为85 dB)、CO染毒组(CO浓度为460 mg/m

)、联合染毒组(CO浓度460 mg/m

、噪声强度85 dB)，每组10只。大鼠固定于口鼻染毒杯并放置于联合模拟染毒舱中，按照染毒流程每天染毒2 h，连续30 d。染毒结束后分析精子质量，包括精子运动能力、精子密度等，通过TUNEL染色确定睾丸细胞凋亡水平。睾丸组织送样进行circRNA测序，测序数据行生物信息学富集分析。

结果

与阴性对照组相比，噪声染毒组和联合染毒组精液密度均显著降低(

＜0.05)。在精子运动能力方面，与阴性对照组相比，CO组显著下降(

＜0.05)，联合染毒组精子运动能力下降更为显著(

＜0.05)。噪声染毒组、CO染毒组大鼠睾丸组织可见少量空泡形成，联合染毒组大鼠睾丸组织多处可见空泡形成，生精小管管腔内细胞排列紊乱。噪声染毒组和CO染毒组细胞凋亡增多，联合染毒组细胞凋亡数目最多。测序数据生物信息学分析结果提示，关键应答circRNA及其调控的关键分子可能参与噪声和CO染毒对大鼠睾丸的损伤效应，包括

Smc

4、

Sycp

1、

Sycp

3、

Spag

17、

Mns

1、

Top

和

Rock

1等，主要参与减数分裂、精子形成、细胞周期及凋亡通路。

结论

噪声和CO可致雄性生殖损伤并存在典型的联合作用，且生殖损伤与circRNAs调控相关。

Abstract

Objective

To explore the male reproductive toxicity in rats caused by single or combined exposure to noise and carbon monoxide (CO) in closed cabin

and evaluate the joint effects and associated signal pathways.

Methods

Fifty male SD rats were randomly divided into blank control group

restraint group (negative control group)

noise exposure group

CO exposure group and combined group (

=10). According to the poisoning procedure

the rats were fixed in the oral and nasal toxic cup and placed in the chamber and they were poisoned for 2 h per day for 30 consecutive days. The sperm quality

including sperm motility and sperm density

was analyzed after exposure

and the apoptosis of testicular cells was determined by TUNEL staining. The testicular tissues were

harvested for circRNA sequencing

and the sequencing data were analyzed by bioinformatics enrichment.

Results

After exposure to noise and CO

the semen density of the noise group and combine group was decreased significantly (

0.05). The sperm motility was obviously lower in the CO group than the negative control group (

0.05)

and more serious decline was observed in the combined group (

0.05). Morphological study showed that there were a few vacuoles in the testis of rats exposed to noise or CO and much more vacuoles in the testis of rats exposed to combination. Obvious pathological damage was found in the testis of rats combined exposed to noise and CO

and the cells in the seminiferous tubules were in a disordered arrangement. The number of apoptotic cells was increased in the noise and CO group

and the number was the largest in the combined group. The results of sequencing analysis suggested that key circRNA and their regulated key molecules might be involved in the reproductive damage effects of noise and CO exposure on rat testes

including

Smc

Sycp

Spag

Mns

Top

2a and

Rock

which played important roles in meiosis

spermatogenesis

cell cycle and apoptosis pathways.

Conclusion

Combined exposure to noise and CO shows typical joint effect on male reproductive damages

which are associated with circRNAs regulation.

关键词

Keywords

references

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