Effects of 50 Hz magnetic field exposure on transcription in Neuro2a/APP(695) cells

Jiawen TAO; Qinlong MA; Mingyue GONG; Chunhai CHEN; Peng GAO; Lei ZHANG; Mindi HE; Yonghui LU; Huifeng PI; Zhengping YU; Yanwen ZHANG

doi:10.16016/j.2097-0927.202210134

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Effects of 50 Hz magnetic field exposure on transcription in Neuro2a/APP(695) cells

Basic Medicine | 更新时间：2026-01-09

- Effects of 50 Hz magnetic field exposure on transcription in Neuro2a/APP(695) cells
- Journal of Army Medical University Vol. 45, Issue 18, Pages: 1894-1906(2023)
- 作者机构：
  
  400038 重庆，陆军军医大学(第三军医大学)军事预防医学系军队劳动卫生学教研室，电磁辐射医学防护教育部重点实验室
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(31770906)
- DOI：10.16016/j.2097-0927.202210134
  CLC： R363.21;R394.3;R594.8
- Received：17 October 2022，
  
  Revised：2023-01-03，
  
  Published：30 September 2023
- 稿件说明：
移动端阅览
Jiawen TAO, Qinlong MA, Mingyue GONG, et al. Effects of 50 Hz magnetic field exposure on transcription in Neuro2a/APP(695) cells[J]. Journal of Army Medical University, 2023, 45(18): 1894-1906.
DOI：

Jiawen TAO, Qinlong MA, Mingyue GONG, et al. Effects of 50 Hz magnetic field exposure on transcription in Neuro2a/APP(695) cells[J]. Journal of Army Medical University, 2023, 45(18): 1894-1906. DOI： 10.16016/j.2097-0927.202210134.

摘要

目的

研究50 Hz磁场暴露对Neuro2a/APP(695)细胞基因转录的影响。

方法

Neuro2a/APP(695) 细胞简单随机分为假性辐照组和磁场暴露组(

=3)。暴露条件为1.0 mT 50 Hz正旋波磁场，4 h/d，连续3 d；假性辐照组细胞除无磁场暴露外，余同磁场暴露组。暴露结束后按标准方法送样，进行转录组测序检测，包括样品RNA提取、数据质量评价和数据分析等。

结果

50 Hz磁场暴露后Neuro2a/APP(695)细胞有141种定性的基因表达发生了明显的变化，其中有129种表达上调、12种表达下调。GO富集分析结果显示，Neuro2a/APP(695)细胞中62种上调差异表达基因涉及432类显著GO功能富集，其中涉及生物学过程337类、细胞组分16类、分子功能79类；3种下调差异表达基因共涉及275类显著GO功能富集，其中涉及生物学过程260类、细胞组分5类、分子功能10类。KEGG通路分析显示，这些差异基因可能参与了20条KEGG通路，其中2个下调差异表达基因通路，18个上调差异表达基因通路。

结论

50 Hz磁场暴露可能对Neuro2a/APP(695)细胞的黏附连接、Hippo信号通路、糖胺聚糖生物合成-硫酸软骨素/硫酸皮肤素、蛋白消化和吸收和甘露糖型O-聚糖生物合成等方面产生影响，以上通路可能是50 Hz磁场对Neuro2a/APP(695)细胞产生影响的主要途径。

Abstract

Objective

To investigate the impact of exposure to 50 Hz magnetic field on transcription in Neuro2a/APP(695) cells.

Methods

Neuro2a/APP(695) cells were divided into radiation group and sham

radiation group (

=3). The cells in the former group were exposed to 50 Hz magnetic field (1 mT

4 h/d) for 3 consecutive days

and those from the latter group were only placed in the same coil system under the same conditions but without 50 Hz magnetic field. After exposure

all samples were collected to undergo standard procedures for transcriptome sequencing

including RNA extraction

data quality evaluation

and data analysis.

Results

There were 141 identified significant differentially expressed genes (DEGs) in Neuro2a/APP(695) cells after exposure

of which 129 were up-regulated and 12 were down-regulated. Gene Ontology (GO) enrichment analysis showed that the 62 up-regulated DEGs were involved in 432 significantly functional properties

of which 337 belong to biological processes

16 to cellular components and 79 to molecular functions. While

the 3 down-regulated DEGs were involved in 275 significantly functional properties

of which 260 belonged to biological processes

5 to cellular components and 10 to molecular functions. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that these DEGs may be involved in 20 differential KEGG pathways

including 2 down-regulated pathways and 18 up-regulated pathways.

Conclusion

Exposure to 50 Hz magnetic field may affect adhesion junction

Hippo signaling pathway

glycosaminoglycan biosynthesis-chondroitin sulfate/dermatan sulfate

protein digestion and absorption

mannose type O-glycan biosynthesis in Neuro2a/APP(695) cells

and these may be the main pathways that the exposure affects the cells.

关键词

Keywords

references

王伟, 王博, 李鑫, 等. 基于GB、IEC、ANSI标准比对及50Hz/60Hz变压器温升试验方法研究[J]. 电工技术, 2022(1): 96-98. DOI: 10.19768/j.cnki.dgjs.2022.01.028.

WANG W, WANG B, LI X, et al . Research on temperature rise test method of 50 Hz/60 Hz transformer based on GB, IEC and ANSIstandard[J ] . Electr Eng, 2022(1): 96-98. DOI: 1 0.19768/j.cnki.dgjs.2022.01.028.

WERTHEIMER N, LEEPER E. Electrical wiring configurations and childhood cancer[J]. Am J Epidemiol, 1979, 109(3): 273-284. DOI: 10.1093/oxfordjournals.aje.a112681.

BEHARI J, RAJAMANI P. Electromagnetic field exposure Effects(ELF-EMF and RFR) on fertility and reproduction[OL ] . [2012-11 ] . https://bioinitiative.org/wp-content/uploads/pdfs/sec18_2012_Exposure_Effects_Fertility_Reproduction.pdf https://bioinitiative.org/wp-content/uploads/pdfs/sec18_2012_Exposure_Effects_Fertility_Reproduction.pdf .

LI D K, ODOULI R, WI S, et al . A population-based prospective cohort study of personal exposure to magnetic fields during pregnancy and the risk of miscarriage[J ] . Epidemiology, 2002, 13(1): 9-20. DOI: 10.1097/00001648-200201000-00004.

LI D K, YAN B, LI Z, et al . Exposure to magnetic fields and the risk of poor sperm quality[J ] . Reprod Toxicol, 2010, 29(1): 86-92. DOI: 10.1016/j.reprotox.2009.09.004.

CHEN Y F, HONG L, ZENG Y, et al . Power frequency magnetic fields induced reactive oxygen species-related autophagy in mouse embryonic fibroblasts[J ] . Int J Biochem Cell Biol, 2014, 57: 108-114. DOI: 10.1016/j.biocel.2014.10.013.

KIM J, HA C S, LEE H J, et al . Repetitive exposure to a 60-Hz time-varying magnetic field induces DNA double-strand breaks and apoptosis in human cells[J ] . Biochem Biophys Res Commun, 2010, 400(4): 739-744. DOI: 10.1016/j.bbrc.2010.08.140.

KIM J, YOON Y, YUN S, et al . Time-varying magnetic fields of 60 Hz at 7 mT induce DNA double-strand breaks and activate DNA damage checkpoints without apoptosis[J ] . Bioelectromagnetics, 2012, 33(5): 383-393. DOI: 10.1002/bem.21697.

VIANALE G, REALE M, AMERIO P, et al . Extremely low frequency electromagnetic field enhances human keratinocyte cell growth and decreases proinflammatory chemokine production[J ] . Br J Dermatol, 2008, 158(6): 1189-1196. DOI: 10.1111/j.1365-2133.2008.08540.x.

WOLF F I, TORSELLO A, TEDESCO B, et al . 50-Hz extremely low frequency electromagnetic fields enhance cell proliferation and DNA damage: possible involvement of a redox mechanism[J ] . Biochim Biophys Acta BBA Mol Cell Res, 2005, 1743(1/2): 120-129. DOI: 10.1016/j.bbamcr.2004.09.005.

JADIDI M, FIROOZABADI S M, RASHIDY-POUR A, et al . Acute exposure to a 50 Hz magnetic field impairs consolidation of spatial memory in rats[J ] . Neurobiol Learn Mem, 2007, 88(4): 38 7-392. DOI: 10.1016/j.nlm.2007.07.010.

LAI J S, ZHANG Y M, LIU X F, et al . Effects of extremely low frequency electromagnetic fields (100 μT) on behaviors in rats[J ] . NeuroToxicology, 2016, 52: 104-113. DOI: 10.1016/j.neuro.2015.11.010.

BARSAM T, MONAZZAM M R, HAGHDOOST A A, et al . Effect of extremely low frequency electromagnetic field exposure on sleep quality in high voltage substations[J ] . J Environ Health Sci Engineer, 2012, 9(1): 15. DOI: 10.1186/1735-2746-9-15.

HUANG J L, TANG T T, HU G C, et al . Association between exposure to electromagnetic fields from high voltage transmission lines and neurobehavioral function in children[J ] . PLoS One, 2013, 8(7): e67284. DOI: 10.1371/journal.pone.0067284.

QIU C X, FRATIGLIONI L, KARP A, et al . Occupational exposure to electromagnetic fields and risk of Alzheimer's disease[J ] . Epidemiology, 2004, 15(6): 687-694. DOI: 10.1097/01.ede.0000142147.49297.9d.

BAI W F, XU W C, FENG Y, et al . Fifty-Hertz electromagnetic fields facilitate the induction of rat bone mesenchymal stromal cells to differentiate into functional neurons[J ] . Cytotherapy, 2013, 15(8): 961-970. DOI: 10.1016/j.jcyt.2013.03.001.

JUNG I S, KIM H J, NOH R, et al . Effects of extremely low frequency magnetic fields on NGF induced neuronal differentiation of PC12 cells[J ] . Bioelectromagnetics, 2014, 35(7): 459-469. DOI: 10.1002/bem.21861.

TASSET I, MEDINA F J, JIMENA I, et al . Neuroprotective effects of extremely low-frequency electromagnetic fields on a Huntington's disease rat model: effects on neurotrophic factors and neuronal density[J ] . Neuroscience, 2012, 209: 54-63. DOI: 10.1016/j.neuroscience.2012.02.034.

聂淑雅, 贾建平. 阿尔茨海默病研究的常用细胞模型[J]. 中华医学杂志, 2022, 102(5): 385-388. DOI: 10.3760/cma.j.cn112137-20210702-01494.

NIE S Y, JIA J P. Common cell models for Alzheimer's disease research[J]. Natl Med J China, 2022, 102(5): 385-388. DOI: 10.3760/cma.j.cn112137-20210702-01494.

吴玉韶. 认知症老年人照护服务需求亟待满足[J]. 中国国情国力, 2021(10): 1. DOI: 10.13561/j.cnki.zggqgl.2021.10.001.

WU Y S. The demand for care services for the elderly with cognitive impairment needs to be met urgently[J]. China Natl Cond Strength, 2021(10): 1. DOI: 10.13561/j.cnki.zggqgl.2021.10.001.

ALISON BRUNIER, ANDREI MUCHNIK. World failing to address dementia challenge[OL ] . [2021-9 ] . https://www.who.int/news/item/02-09-2021-world-failing-to-address-dementia-challenge https://www.who.int/news/item/02-09-2021-world-failing-to-address-dementia-challenge .

DOMINICI L, VILLARINI M, FATIGONI C, et al . Genotoxic hazard evaluation in welders occupationally exposed to extremely low-frequency magnetic fields (ELF-MF)[J ] . Int J Hyg Environ Health, 2011, 215(1): 68-75. DOI: 10.1016/j.ijheh.2011.07.010.

HAVAS M. When theory and observation collide: can non-ionizing radiation cause cancer?[J]. Environ Pollut, 2017, 221: 501-505. DOI: 10.1016/j.envpol.2016.10.018.

MCCANN J, DIETRICH F, RAFFERTY C. The genotoxic potential of electric and magnetic fields: an update[J]. Mutat Res Mutat Res, 1998, 411(1): 45-86. DOI: 10.1016/S1383-5742(98)00006-4.

VERSCHAEVE L, WAMBACQ S, ANTHONISSEN R, et al . Co-exposure of ELF-magnetic fields and chemical mutagens: an investigation of genotoxicity with the SOS-based VITOTOX test in Salmonella typhimurium [J ] . Mutat Res Toxicol Environ Mutagen, 2016, 795: 31-35. DOI: 10.1016/j.mrgentox.2015.11.003..

VIJAYALAXMI, PRIHODA T J. Genetic damage in mammalian somatic cells exposed to extremely low frequency electro-magnetic fields: a meta-analysis of data from 87 publications (1990-2007)[J]. Int J Radiat Biol, 2009, 85(3): 196-213. DOI: 10.1080/09553000902748575.

ZHANG D, ZHANG Y, ZHU B Y, et al . Resveratrol may reverse the effects of long-term occupational exposure to electromagnetic fields on workers of a power plant[J ] . Oncotarget, 2017, 8(29): 47497-47506. DOI: 10.18632/oncotarget.17668.

秦雅鑫, 宋琦, 殷樱, 等. 电磁场在转录水平对胚胎干细胞源性神经前体细胞凋亡相关基因的影响[J]. 第四军医大学学报, 2008, 29(10): 876-879. DOI: 10.3321/j.issn: 1000-2790.2008.10.004.

QIN Y X, SONG Q, YIN Y, et al . Effect of electromagnetic fields on transcript levels of apoptosis-related genes in embryonic stem cell-derived neural progenitor cells[J ] . J Fourth Mil Med Univ, 2008, 29(10): 876-879. DOI: 10.3321/j.issn: 1000-2790.2008.10.004.

朱茂林, 朱煌. 极低频电磁场辐射干扰后小鼠成纤维细胞转录组测序的生物信息学分析[J]. 中华实验眼科杂志, 2021, 39(8): 686-692. DOI: 10.3760/cma.j.cn115989-20200905-00633.

ZHU M L, ZHU H. Bioinformatics analysis of transcriptome sequencing after the interference of extremely low frequency electromagnetic field in mouse fibroblasts[J]. Chin J Exp Ophthalmol, 2021, 39(8): 686-692. DOI: 10.3760/cma.j.cn115989-20200905-00633.

NAIMA C, AGATHE M, PATRICE R, et al . AβPP-induced UPR transcriptomic signature of glial cells to oxidative stress a s an adaptive mechanism to preserve cell function and survival[J ] . Curr Alzheimer Res, 2018, 15(7): 643-654.

ZHANG Y, CHEN D D, ZHANG M, et al . Treadmill training attenuate STZ-induced cognitive dysfunction in type 2 diabetic rats via modulating Grb10/IGF-R signaling[J ] . Brain Res Bull, 2022, 181: 12-20. DOI: 10.1016/j.brainresbull.2022.01.007.

CHAPUIS J, VINGTDEUX V, CAMPAGNE F, et al . Growth arrest-specific 1 binds to and controls the maturation and processing of the amyloid-beta precursor protein[J ] . Hum Mol Genet, 2011, 20(10): 2026-2036. DOI: 10.1093/hmg/ddr085.

BRADLEY-WHITMAN M A, LOVELL M A. Epigenetic changes in the progression of Alzheimer's disease[J]. Mech Ageing Dev, 2013, 134(10): 486-495. DOI: 10.1016/j.mad.2013.08.005.

SYAL C, KOSARAJU J, HAMILTON L, et al . Dysregulated expression of monoacylglycerol lipase is a marker for anti-diabetic drug metformin-targeted therapy to correct impaired neurogenesis and spatial memory in Alzheimer's disease[J ] . Theranostics, 2020, 10(14): 6337-6360. DOI: 10.7150/thno.44962.

贾建平, 贾健民, 周卫东, 等. 阿尔茨海默病和血管性痴呆患者脑脊液中乙酰胆碱和胆碱检测及其临床意义[J]. 中华神经科杂志, 2002, 35(3): 168-170. DOI: 10.3760/j.issn: 1006-7876.2002.03.013.

JIA J P, JIA J M, ZHOU W D, et al . The detection of cerebrospinal fluid acetylecholine and choline in patient with Alzheimer's disease and vascular dementia and their clinical significance[J ] . Chin J Neurol, 2002, 35(3): 168-170. DOI: 10.3760/j.issn: 1006-7876.2002.03.013.

IKARASHI Y, HARIGAYA Y, TOMIDOKORO Y, et al . Decreased level of brain acetylcholine and memory disturbance in APPsw mice[J ] . Neurobiol Aging, 2004, 25(4): 483-490. DOI: 10.1016/S0197-4580(03)00122-2.

AFRASIABI A, RIAZI G H, ABBASI S, et al . Synaptosomal acetylcholinesterase activity variation pattern in the presence of electromagnetic fields[J ] . Int J Biol Macromol, 2014, 65: 8-15. DOI: 10.1016/j.ijbiomac.2014.01.006.

LI Y Q, DENG P, CHEN C H, et al . 1, 800 MHz radiofrequency electromagnetic irradiation impairs neurite outgrowth with a decrease in Rap1-GTP in primary mouse hippocampal neurons and Neuro2a cells[J ] . Front Public Health, 2021, 9: 771508. DOI: 10.3389/fpubh.2021.771508.

HU L T, ZHU B L, LAI Y J, et al . HMGCS 2 promotes autophagic degradation of the amyloid-β precursor protein through ketone body-mediated mechanisms[J ] . Biochem Biophys Res Commun, 2017, 486(2): 492-498. DOI: 10.1016/j.bbrc.2017.03.069..

CASTILLO G M, LUKITO W, WIGHT T N, et al . The sulfate moieties of glycosaminoglycans are critical for the enhancement of β-amyloid protein fibril formation[J ] . J Neurochem, 1999, 72(4): 1681-1687. DOI: 10.1046/j.1471-4159.1999.721681.x.

OREŠIC ˇ M, HYÖTYLÄINEN T, HERUKKA S K, et al . Metabolome in progression to Alzheimer's disease[J ] . Transl Psychiatry, 2011, 1(12): e57. DOI: 10.1038/tp.2011.55.

JOHNSON R J, GOMEZ-PINILLA F, NAGEL M, et al . Cerebral fructose metabolism as a potential mechanism driving alzheimer's disease[J ] . Front Aging Neurosci, 2020, 12: 560865. DOI: 10.3389/fnagi.2020.560865.

GUO X Z, TANG P, LIU P, et al . Dkk1: a promising molecule to connect Alzheimer's disease and osteoporosis[J ] . Med Hypotheses, 2016, 88: 30-32. DOI: 10.1016/j.mehy.2015.12.023.

ZHANG L, SUN C X, JIN Y X, et al . Dickkopf 3 (Dkk3) improves amyloid-β pathology, cognitive dysfunction, and cerebral glucose metabolism in a transgenic mouse model ofalzheimer's disease[J ] . J Alzheimer Dis, 2017, 60(2): 733-746. DOI: 10.3233/jad-161254.

王芙蓉. NOTCH信号传导途径与阿尔茨海默病[J]. 国外医学: 老年医学分册, 2002, 23(2): 49.

WANG F Y. NOTCH signal transduction pathway and Alzheimer disease[J]. Foreign Med: Geriatr, 2002, 23 (2): 49.

才岩. Wnt信号传导通路在阿尔茨海默病中的研究进展[J]. 中国社区医师, 2016, 32(24): 13-13, 15. DOI: 10.3969/j.issn.1007-614x.2016.24.4.

CAI Y. Research progress of Wnt signal transduction pathway in alzheimer disease[J]. Chin Community Dr, 2016, 32(24): 13-13, 15. DOI: 10.3969/j.issn.1007-614x.2016.24.4.

LU N H, WANG J Z, ZHU B J, et al . Whole-exome sequencing to identify novel mutations of nevoid basal cell carcinoma syndrome in a Chinese population[J ] . Cancer Biomark, 2017, 21(1): 161-168. DOI: 10.3233/cbm-170541.

YASUHARA R, KANG S Y, IRIÉ T, et al . Role of Snai2 and Notch signaling in salivary gland myoepithelial cell fate[J ] . Lab Investig, 2022, 102(11): 1245-1256. DOI: 10.1038/s41374-022-00814-7.

LI J J, PERFETTO M, MATERNA C, et al . A new transgenic reporter line reveals Wnt-dependent Snai2 re-expression and cranial neural crest differentiation in Xenopus [J ] . Sci Rep, 2019, 9(1): 1-14. DOI: 10.1038/s41598-019-47665-9..

WOOD AMY H R, CHAPPELL HELEN F, ZULYNIAK MICHAEL A. Dietary and supplemental long-chain omega-3 fatty acids as moderators of cognitive impairment and Alzheimer's disease[J]. Eur J Nutr, 2021, 61(2): 1-16.

高梅梅. 阿尔茨海默病及轻度认知功能障碍患者血清代谢组学研究[D]. 济南: 山东大学, 2017.

GAO M M. Study on serum metabonomics in patients with Alzheimer's disease and mild cognitive impairment[D]. Jinan: Shandong University, 2017.

赵娜. 不同分子量和硫酸化程度肝素组分的抗阿尔茨海默病活性研究[D]. 济南: 山东大学, 2014.

ZHAO N. Study on anti-Alzheimer's disease activity of heparin fractions with different molecular weight and sulfated degree[D]. Jinan: Shandong University, 2014.

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