Expression and role of PD-L1 in colonic mucosa of ulcerative colitis patients and DSS-induced colitis mouse model

Yalan SONG; Tianyu WU; Cong CHEN; Zhenyu WU; Xudong TANG; Lei CHEN

doi:10.16016/j.2097-0927.202210100

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Expression and role of PD-L1 in colonic mucosa of ulcerative colitis patients and DSS-induced colitis mouse model

Basic Medicine | 更新时间：2026-01-09

- Expression and role of PD-L1 in colonic mucosa of ulcerative colitis patients and DSS-induced colitis mouse model
- Journal of Army Medical University Vol. 45, Issue 2, Pages: 155-164(2023)
- 作者机构：
  
  400038 重庆，陆军军医大学(第三军医大学)第一附属医院消化内科
- 作者简介：
- 基金信息：
  
  by the General Program of National Natural Science Foundation of China(81470850)
- DOI：10.16016/j.2097-0927.202210100
  CLC： R361.2;R392.32;R574.62
- Received：13 October 2022，
  
  Revised：2022-12-06，
  
  Published：30 January 2023
- 稿件说明：
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Yalan SONG, Tianyu WU, Cong CHEN, et al. Expression and role of PD-L1 in colonic mucosa of ulcerative colitis patients and DSS-induced colitis mouse model[J]. Journal of Army Medical University, 2023, 45(2): 155-164.
DOI：

Yalan SONG, Tianyu WU, Cong CHEN, et al. Expression and role of PD-L1 in colonic mucosa of ulcerative colitis patients and DSS-induced colitis mouse model[J]. Journal of Army Medical University, 2023, 45(2): 155-164. DOI： 10.16016/j.2097-0927.202210100.

摘要

探讨细胞程序性死亡配体-1(programmed cell death-ligand 1

PD-L1)在溃疡性结肠炎(ulcerative colitis

UC)发病机制中的作用。

方法

收集2022年1-9月于陆军军医大学第一附属医院消化内科肠镜室就诊的18~64岁的UC患者结肠组织标本29例

同期收集非UC患者结肠组织标本22例

采用免疫组化和RT-qPCR检测

1和炎症因子

-1

、

-6、

-17

、

-22、

TGF

1的表达。选取8~10周龄C57BL/6雄性小鼠24只(23~26 g)

分为空白对照组(

=4)、PD-L1-Fc组(

=4)、葡聚糖硫酸钠(dextran sulfate sodium salt

DSS)组(

=8)、DSS+PD-L1-Fc组(

=8)

DSS组及DSS+PD-L1-Fc组连续7天予以2.5% DSS溶液自由饮水

第8天更换为正常饮水

其余组

正常饮水

第3天予以PD-L1-Fc组及DSS+PD-L1-Fc组小鼠腹腔注射PD-L1-Fc蛋白(1 mg/mL)40μg/只

造模后8~10天检测各组小鼠结肠黏膜中PD-L1的表达情况

并观察各组小鼠的体质量、粪便性状、活体结肠黏膜形态变化、组织病理学改变及炎症因子改变情况。

结果

与对照组相比

UC患者结肠黏膜中PD-L1表达显著升高(43.03±4.044

0.001)

促炎因子

-1

、

-6及抗炎因子

-22、

TGF

1的表达均升高(

0.05);在DSS小鼠模型中同样存在这一现象。腹腔注射PD-L1-Fc蛋白后

DSS小鼠的体质量、粪便评分、结肠黏膜大体形态及显微镜下炎症程度明显改善(

0.05)

-6、

-17

下调(

0.05)

-22、

Foxp

3上调(

0.05)。

结论

1在UC患者及小鼠DSS结肠炎模型中升高

可能是机体的一种适应性保护作用。

Abstract

Objective

To explore the role of programmed cell death-ligand 1(PD-L1) in pathogenesis of ulcerative colitis (UC).

Methods

A total of 29 colon tissue samples from UC patients aged 18~64 years who underwent the colonoscopy in our department from January 2022 to September 2022 were collected

and another 22 colon tissue samples from non-UC patients were also collected during the same period.The expression of

1 and inflammatory factors

-1

-6

-17

-22 and

TGF

1 were detected by immunohistochemistry and RT-qPCR.Twenty-four male C57BL/6 mice (8~10 weeks old

23~26 g) were randomly divided into blank control group (

=4)

PD-L1-Fc group (

=4)

dextran sulfate sodium salt (DSS) group (

=8)

and DSS+PD-L1-Fc group (

=8).The mice from the DSS group and DSS+PD-L1-Fc group were given 2.5% DSS solution for 7 consecutive days

and were chang

ed to normal drinking water on the 8th day.The animals of the other groups were given normal drinking water.On the 3rd day

the mice in the PD-L1-Fc group and DSS+PD-L1-Fc group were injected intraperitoneally with PD-L1-Fc protein (1 mg/mL

40 μg/animal).In 8~10 d after modeling

the expression of

1 in colonic mucosa was detected

and the body weight

fecal traits

morphological changes

histopathological changes and inflammatory factors were observed in different groups.

Results

The expression of

1 was significantly increased in the colonic mucosa of UC patients (43.03±4.044

0.001) than of normal colon tissue samples

and the expression of pro-inflammatory factors

-1

and

-6 and anti-inflammatory factors

-22 and

TGF

1 were increased (

0.05)

which also existed in DSS mouse models.Intraperitoneal injection of PD-L1-Fc protein improved body weight

fecal score

gross morphology of colonic mucosa and microscopic inflammation (

0.05)

down-regulated

-6 and

-17

(

0.05) and up-regulated

-22 and

Foxp

0.05) in the DSS+PD-L1-Fc mice when compared with the DSS group.

Conclusion

The upregulation of

1 in UC patients and DSS-induced colitis mouse models may play an adaptive protective role.

关键词

Keywords

references

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