Effect of Musculin on biological function of mouse bone marrow-derived dendritic cells

Leiting ZHANG; Yu XIA; Jing YU; Ke LIU; Huimin CHONG; Suiyan LI; Jun YAN

doi:10.16016/j.2097-0927.202209153

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Effect of Musculin on biological function of mouse bone marrow-derived dendritic cells

Basic Medicine | 更新时间：2026-01-09

- Effect of Musculin on biological function of mouse bone marrow-derived dendritic cells
- Journal of Army Medical University Vol. 45, Issue 6, Pages: 539-546(2023)
- 作者机构：
  
  1.610031 成都，西南交通大学生命科学与工程学院
  2.400042 重庆，陆军特色医学中心野战外科研究部，创伤、烧伤与复合伤国家重点实验室，特殊环境战伤防治研究室
  3.400042 重庆，陆军特色医学中心检验科
- 作者简介：
- 基金信息：
  
  the Open Project of Key Laboratory of Extreme Environmental Medicine of Ministry of Education of China(PR-KL2020GY004)
- DOI：10.16016/j.2097-0927.202209153
  CLC： R392.1;R394.3
- Received：16 September 2022，
  
  Revised：2022-10-14，
  
  Published：30 March 2023
- 稿件说明：
移动端阅览
Leiting ZHANG, Yu XIA, Jing YU, et al. Effect of Musculin on biological function of mouse bone marrow-derived dendritic cells[J]. Journal of Army Medical University, 2023, 45(6): 539-546.
DOI：

Leiting ZHANG, Yu XIA, Jing YU, et al. Effect of Musculin on biological function of mouse bone marrow-derived dendritic cells[J]. Journal of Army Medical University, 2023, 45(6): 539-546. DOI： 10.16016/j.2097-0927.202209153.

摘要

目的

探讨新型转录因子Musculin对小鼠骨髓来源树突状细胞(bone marrow-derived dendritic cells

BMDC)生物学功能的影响。

方法

获取野生型(wildtype

WT)和Musculin敲除(knockout

-/-)小鼠骨髓细胞，用粒细胞-巨噬细胞集落刺激因子(granulocyte-macrophage colony stimulating factor

GM-CSF)和IL-4体外诱导BMDC，7 d后加入脂多糖(lipopolysaccharide

LPS)，继续培养24 h；动态观察细胞形态变化，收集WT组和Musculin-/-组BMDC及上清，流式细胞术分析各组细胞表达CD11c、CD40、CD80、CD86、PD-L1和IAIE的比例，CCK-8法检测各组细胞刺激异源淋巴细胞增殖情况，并计算刺激指数(stimulation indexes

SI)，ELISA法检测各组细胞上清中IL-1β、IL-6、IL-10、IL-12(P40)、IL-12(P70)和TNF-α的水平。

结果

与WT组比较，Musculin-/-组BMDC形态相似，纯度无明显差异；表面共刺激分子CD40、CD80和CD86的比例显著下降(

0.05)，共抑制分子PD-L1和MHC-Ⅱ分子IAIE的比例也降低，但差异无统计学意义；刺激异源淋巴细胞后的数量和刺激指数上升，尤其是刺激5 d以后(

0.05)；上清中促炎因子IL-1β、IL-6、IL-12(P40)、IL-12(P70)和TNF-α水平显著升高(

0.05)，但抗炎因子IL-10的水平变化不明显。

结论

Musculin可能增强BMDC介导的免疫功能并抑制炎症反应，提示Musculin能够调控BMDC的生物学功能。

Abstract

Objective

To investigate the effect of a novel transcription factor

Musculin

on the biological functions of bone marrow-derived dendritic cells (BMDC) in mice.

Methods

Bone marrow cells from wildtype (WT) and Musculin knockout (KO

-/-) mice were obtained and cultured with granulocyte-macrophage colony stimulating factor (GM-CSF) and IL-4 so as to induce

BMDC

in vitro

. After 7 d

the BMDC were added with lipopolysaccharide (LPS) and continued to culture for 24 h. Besides the cellular morphological changes were observed dynamically

BMDC and the supernatants were collected from the WT and Musculin-/-cells. Flow cytometry was used to analyze the proportion of positive cells of CD11c

CD40

CD80

CD86

PD-L1 and IAIE in each group. CCK-8 assay was adopted to detect the cellular proliferation for stimulating heterologous lymphocytes and the different stimulation indexes (SI) were calculated respectively. ELISA was applied to detect the supernatant contents of IL-1β

IL-6

IL-10

IL-12 (P40)

IL-12 (P70) and TNF-α in each group.

Results

The BMDC from the Musculin-/-group had similar morphology and no significant difference in purity

decreased percentages of co-stimulatory molecules such as CD40

CD80 and CD86 (

0.05)

and reduced percentages of co-inhibitory molecules PD-L1 and major histocompatibility complex class Ⅱ (MHC-Ⅱ) IAIE though no significant differences when compared with those of WT group. Moreover

the number of heterologous lymphocytes and related SI after of mixed lymphocyte reaction (MLR) were obvious higher in the Musculin-/-group than the WT group

especially after 5 days' stimulation (

0.05). In addition

the supernatant levels of proinflammatory factors including IL-1β

IL-6

IL-12 (P40)

IL-12 (P70) and TNF-α were significantly increased in the Musculin-/-group than the WT group (

0.05)

but no such changes were observed in anti-inflammatory factor IL-10 level between the 2 groups.

Conclusion

Musculin may enhance the immune function mediated by BMDC and suppress inflammatory reaction

suggesting that it can regulate the biological functions on BMDC.

关键词

Keywords

references

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