Running exercise shows anti-depressive effect in mice through PV<sup>+</sup> interneurons in medial prefrontal cortex

Lu QIN; Jing TANG; Xin LIANG; Yingqiang QI; Yanmin LUO; Qian XIAO; Dujuan HUANG; Mei ZHOU; Yuning ZHOU; Yong TANG

doi:10.16016/j.2097-0927.202208205

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Running exercise shows anti-depressive effect in mice through PV⁺ interneurons in medial prefrontal cortex

Basic Medicine | 更新时间：2026-01-09

- Running exercise shows anti-depressive effect in mice through PV⁺ interneurons in medial prefrontal cortex
- Journal of Army Medical University Vol. 45, Issue 3, Pages: 209-218(2023)
- 作者机构：
  
  1.400016 重庆，重庆医科大学基础医学院组织与胚胎学教研室
  2.400016 重庆，重庆医科大学基础医学院病理生理学教研室
  3.400016 重庆，重庆医科大学生命科学研究院
  4.400016 重庆，重庆医科大学基础医学院生理学教研室
  5.400016 重庆，重庆医科大学基础医学院放射医学教研室
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(82171522);the National Natural Science Foundation for Young Scholars of China(82001436)
- DOI：10.16016/j.2097-0927.202208205
  CLC： R322.81;R455;R749.4
- Received：29 August 2022，
  
  Revised：2022-11-04，
  
  Published：15 February 2023
- 稿件说明：
移动端阅览
Lu QIN, Jing TANG, Xin LIANG, et al. Running exercise shows anti-depressive effect in mice through PV⁺ interneurons in medial prefrontal cortex[J]. Journal of Army Medical University, 2023, 45(3): 209-218.
DOI：

Lu QIN, Jing TANG, Xin LIANG, et al. Running exercise shows anti-depressive effect in mice through PV⁺ interneurons in medial prefrontal cortex[J]. Journal of Army Medical University, 2023, 45(3): 209-218. DOI： 10.16016/j.2097-0927.202208205.

摘要

目的

探讨跑步对慢性不可预知性(chronic unpredictable stress，CUS)抑郁症模型小鼠抑郁样行为的影响和内侧前额叶皮质(medial prefrontal cortex，mPFC)内GABA能中间神经元、PV

中间神经元和GAD67

/PV

细胞比例的影响。

方法

选取4~6周龄的雄性C57BL/6小鼠，CUS建模成功后，利用糖水偏好实验结果将其以分层随机法分为对照组、CUS模型对照组和CUS模型+跑步组。运用糖水偏好实验、强迫游泳实验和悬尾实

验评估各组小鼠的行为学水平。采用qRT-PCR检测小鼠mPFC中GAD1、GAD2、PV和CCK的mRNA表达水平。采用免疫组织化学和现代体视学相结合的方法对小鼠mPFC内GABA能中间神经元和PV

中间神经元数量进行精确三维定量，并分析PV

/GAD67

细胞比例。

结果

与CUS模型对照组小鼠相比，CUS模型+跑步组小鼠和对照组的糖水偏好百分比显著增加(

0.05)，强迫游泳不动时间显著降低(

0.05)，悬尾不动时间显著降低(

0.05)；对照组、CUS模型对照组和CUS模型+跑步组小鼠mPFC内GAD1、GAD2和CCK的mRNA表达水平以及GABA能中间神经元数量差异无统计学意义(

0.05)；CUS模型对照组小鼠mPFC中PV的mRNA表达水平、PV

中间神经元数量和PV

/GAD67

细胞比例均显著低于CUS模型+跑步组和对照组(

0.05)。

结论

跑步可能通过调节PV

中间神经元的数量和基因表达从而发挥抗抑郁效应。

Abstract

Objective

To investigate the effect of running exercise on depression-like behaviors in chronic unpredictable stress (CUS)-induced depression mice and GABAergic interneurons

interneurons and ratio of GAD67

/PV

cells in medial prefrontal cortex (mPFC).

Methods

After mouse model of depression was successfully established through CUS

these male C57BL/6 mice aged 4~6 weeks were randomly divided into control group

CUS control group and CUS+running group according to the results of sucrose preference test. The behaviors of the 3 groups were evaluated with sucrose preference test

forced swimming test and tail suspension test. The mRNA expression levels of GAD1

GAD2

PV and CCK in mPFC of mice were detected using qRT-PCR. The number of GABAergic interneurons and PV

interneurons in mPFC of mice were quantified using immunohistochemical assay and unbiased stereological technique

and the ratio of PV

/GAD67

cells was calculated.

Results

Compared with those in CUS control group

the mice from the CUS+running and control groups showed a significantly higher percentage of sucrose preference (

0.05)

orter forced swimming immobility time (

0.05)

and decreased tail suspension immobility time (

0.05). There were no obvious differences in mRNA expression levels of GAD1

GAD2 and CCK and number of GABAergic interneurons in mPFC in the 3 groups (P

0.05). The mRNA level of PV

number of PV

interneurons and ratio of PV

/GAD67

cells in mPFC were remarkably lower in the CUS group than the CUS +running group and control group (

0.05).

Conclusion

Running exercise may exert anti-depressive effects through regulating the number and gene expression of PV

interneurons.

关键词

Keywords

references

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⁰

Running exercise shows anti-depressive effect in mice through PV+ interneurons in medial prefrontal cortex

DOI：10.16016/j.2097-0927.202208205

摘要

Abstract

关键词

Keywords

references

Running exercise shows anti-depressive effect in mice through PV⁺ interneurons in medial prefrontal cortex