Effects of acute predator odor exposure on paraventricular thalamus-central amygdala pathway activity in mice

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Effects of acute predator odor exposure on paraventricular thalamus-central amygdala pathway activity in mice

Basic Medicine | 更新时间：2026-01-09

- Effects of acute predator odor exposure on paraventricular thalamus-central amygdala pathway activity in mice
- Journal of Army Medical University Vol. 44, Issue 17, Pages: 1677-1686(2022)
- 作者机构：
  
  1.400050 重庆，重庆医科大学附属康复医院
  2.401331 重庆，重庆医科大学附属大学城医院神经科
  3.400038 重庆，陆军军医大学(第三军医大学)基础医学院生理学教研室
  4.400042 重庆，陆军特色医学中心医学心理科
- 作者简介：
- 基金信息：
  
  the National Natural Science Foundation of China(81971241);the National Natural Science Foundation of China(32000713);the Major Project of Science and Technology Innovation 2030-"Brain Science and Brain-like Research"(2022ZD0205600)
- DOI：10.16016/j.2097-0927.202205139
  CLC： R338.24;R338.26;R338.61
- Received：25 May 2022，
  
  Revised：2022-06-29，
  
  Published：15 September 2022
- 稿件说明：
移动端阅览
Chengyu LIU, Juanjuan ZHAO, Yaling WANG, et al. Effects of acute predator odor exposure on paraventricular thalamus-central amygdala pathway activity in mice[J]. Journal of Army Medical University, 2022, 44(17): 1677-1686.
DOI：

Chengyu LIU, Juanjuan ZHAO, Yaling WANG, et al. Effects of acute predator odor exposure on paraventricular thalamus-central amygdala pathway activity in mice[J]. Journal of Army Medical University, 2022, 44(17): 1677-1686. DOI： 10.16016/j.2097-0927.202205139.

摘要

目的

2

探讨小鼠在急性暴露于捕食者气味——2

4

5-三甲基噻唑(2

4

5-trimethylthiazole，TMT)应激下对丘脑室旁核(paraventricular thalamic nucleus，PVT)- 中央杏仁核(central amygdala，CeA)神经通路活动的影响。

方法

2

取3只同种系小鼠在CeA注射CTB555，观察丘脑中线核团中投射到CeA的神经元；8只雄性C57BL/6小鼠按照简单随机抽样法分为生理盐水组、TMT(暴露于捕食者气味)组，每组4只，观察急性暴露于TMT应激对小鼠丘脑中线核团及杏仁核的c-Fos表达影响；取6只同种系小鼠在CeA注射RetroBeads，随后按照简单随机抽样法分为生理盐水组、TMT组，每组3只，观察急性暴露于TMT对CeA投射-PVT神经元c-Fos表达影响。取6只同种系小鼠在PVT注射AAV-EF1α-DIO-GCaMp7b、CeA注射AAV/retro-Syn-Cre，待病毒表达3周后，通过向PVT病毒注射位点上方埋置光纤，手术恢复后在体记录小鼠CeA投射-PVT神经元急性暴露于TMT前后的钙信号变化。

结果

2

通过向小鼠CeA注射CTB555，发现CeA选择性接受丘脑中线核团大量的PVT投射。与对照组相比，急性暴露于TMT应激下，PVT(

P

<

0.01)及CeA(

P

<

0.05)的c-Fos表达显著增加。小鼠CeA注射RetroBeads后，CeA投射-PVT神经元暴露于TMT后c-Fos表达显著增加(

P

<

0.05)。光纤钙信号记录发现，CeA投射-PVT神经元暴露于TMT后的ΔF/F显著高于暴露于生理盐水的ΔF/F值(

P

<

0.01)。

结论

2

CeA投射-PVT神经元在急性暴露于TMT下显著激活。

Abstract

Objective

2

To investigate the effects of acute exposure to predator odor (2

4

5-trimethylthiazole

TMT) on the activity of the paraventricular thalamic nucleus (PVT)-central amygdala (CeA) neural pathway in mice.

Methods

2

CTB555 was injected into CeA in 3 mice of the same lineage

and the neurons projected to CeA in the midline thalamus nuclei were observed. Eight male C57BL/6 mice were randomly divided into normal saline group and TMT group (exposed to predator odor)

with 4 mice in each group. The effects of acute exposure to TMT on c-Fos expression in the midline thalamus nuclei and amygdala of mice were observed. Six mice of the same lineage were injected with RetroBeads in CeA

and then randomly and equally divided into normal saline group and TMT group. The effects of acute exposure to TMT on c-Fos expression in CeA projecting-PVT neurons were observed. The changes in calcium signals of CeA projecting-PVT neurons

in vivo

under acute exposure to TMT were recorded. AAV-EF1α-DIO-GCaMp7b was injected in PVT and AAV/retro-Syn-Cre was injected in CeA of 6 mice of the same lineage. In 3 weeks later

optical fiber was embedded above the injection site of PVT to

in vivo

record the changes in calcium signals of CeA projecting-PVT neurons before and after acute exposure to TMT.

Results

2

By injecting CTB555 into CeA

it was found that CeA selectively receives a large number of PVT projections in the midline thalamus nuclei. Compared with the control group

the expression of c-Fos in PVT (

P

<

0.01) and CeA (

P

<

0.05) was significantly increased after acute exposure to TMT. After injection RetroBeads in CeA

c-Fos expression in CeA projecting-PVT neurons was significantly enhanced after exposure to TMT (

P

<

0.05). Fiber photometry revealed that the ΔF/F values of CeA projecting-PVT neurons exposed to TMT were significantly higher than those exposed to normal saline (

P

<

0.01).

Conclusion

2

CeA projecting-PVT neurons are activated significantly after acute exposure to TMT.

关键词

Keywords

references

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