Activity patterns of the midline thalamus nuclei-central amygdala pathway during wake/sleep cycle

Juanjuan ZHAO; Chengyu LIU; Xueqi SUN; Mengting CHEN; Qingling HUANG; Ting ZHANG; Shuancheng REN; Dong GAO; Ling TANG

doi:10.16016/j.2097-0927.202205138

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Activity patterns of the midline thalamus nuclei-central amygdala pathway during wake/sleep cycle

Basic Medicine | 更新时间：2026-01-09

- Activity patterns of the midline thalamus nuclei-central amygdala pathway during wake/sleep cycle
- Journal of Army Medical University Vol. 44, Issue 17, Pages: 1694-1704(2022)
- 作者机构：
  
  1.401331 重庆，重庆医科大学附属大学城医院神经科
  2.400050 重庆，重庆医科大学附属康复医院神经科
  3.400038 重庆，陆军军医大学(第三军医大学)基础医学院生理学教研室
  4.400042 重庆，陆军特色医学中心医学心理科
- 作者简介：
- 基金信息：
  
  the National Natural Science Foundation of China(81971241);the National Natural Science Foundation of China(32000713);the Major Project of Science and Technology Innovation 2030-"Brain Science and Brain-like Research"(2022ZD0205600)
- DOI：10.16016/j.2097-0927.202205138
  CLC： R338.24;R338.26;R338.63
- Received：25 May 2022，
  
  Revised：2022-06-29，
  
  Published：15 September 2022
- 稿件说明：
移动端阅览
Juanjuan ZHAO, Chengyu LIU, Xueqi SUN, et al. Activity patterns of the midline thalamus nuclei-central amygdala pathway during wake/sleep cycle[J]. Journal of Army Medical University, 2022, 44(17): 1694-1704.
DOI：

Juanjuan ZHAO, Chengyu LIU, Xueqi SUN, et al. Activity patterns of the midline thalamus nuclei-central amygdala pathway during wake/sleep cycle[J]. Journal of Army Medical University, 2022, 44(17): 1694-1704. DOI： 10.16016/j.2097-0927.202205138.

摘要

目的

探讨中线丘脑各神经核团与杏仁核不同亚区之间的形态学联系特征，以及中线丘脑-中央杏仁核通路在自然觉醒/睡眠周期中的活动模式。

方法

利用逆行神经示踪技术，鉴定中线丘脑主要神经核团与杏仁核不同亚区之间的形态学联系特征；采用光纤记录结合脑电/肌电记录，研究中线丘脑-中央杏仁核通路在觉醒/睡眠期间及状态转换时的活动特征。分别在SPF级健康成年C57BL/6J小鼠杏仁核的两个主要亚区—中央杏仁核(central amygdala，CeA)和基底外侧杏仁核(basolateral amygdala，BLA)注射逆行示踪染料RetroBeads，观察中线丘脑神经核团—丘脑室旁核(paraventricular thalamic nucleus，PVT)、丘脑中央内侧核(central medial thalamic nucleus，CM)、菱形核与连接核(rhomboid thalamic nucleus，Rh/reuniens thalamic nucleus，Re)中RetroBeads阳性胞体分布情况。利用光纤记录结合脑电/肌电记录技术观察特异性投射至CeA的中线丘脑神经元在觉醒/睡眠周期中的活动模式。

结果

逆行神经示踪实验发现，CeA和BLA均接受来自中线丘脑的投射，且接受PVT的投射较多；其中CeA逆行追踪到PVT的神经元数量最多。光纤记录和脑电/肌电记录实验表明，PVT-CeA通路在觉醒期活跃(

0.05)，CM-CeA与Rh/Re-CeA通路在觉醒期和REM睡眠期活跃(

0.05)，Rh/Re-CeA通路总体活动水平较低。

结论

中线丘脑的PVT选择性投射至杏仁核

PVT-CeA通路在觉醒期最为活跃，CM-CeA通路在觉醒期和快速眼动睡眠期活跃，在快速眼动睡眠期更活跃。

Abstract

Objective

To investigate the morphological characteristics of the interconnections between the midline thalamic nucleus and different subregions of the amygdala

and the activity patterns of the midline thalamus-central amygdala pathway during the natural wake/sleep cycle.

Methods

Retrograde nerve tracer technique was used to identify the morphological characteristics of the main nerve nuclei in the midline thalamus and different subregions of amygdala. The activity characteristics of the midline thalamic-central amygdala pathway during wakefulness and sleep and their transition were studied by using optical fiber recording combined with EEG/EMG recording. Retrograde tracer dye RetroBeads were injected into the central amygdala (CeA) and basolateral amygdala (BLA) in the SPF healthy adult C57BL/6J mice. The distribution of RetroBeads positive neurons in paraventricular thalamic nucleus (PVT)

central medial thalamic nucleus (CM) and rhomboid thalamic nucleus/reuniens thalamic nucleus (Rh/Re) in the midline thalamus were observed. The activity patterns of midline thalamic neurons specifically projected to CeA during the wake/sleep cycle were observed by using fiber optic recording combined with EEG/EMG recording techniques.

Results

The retrograde neural tracer test showed that CeA and BLA received projections from the midline thalamus

and PVT received more projections. The number of neurons retrograde from CeA to PVT was the largest. Fiber optic recording and EEG/EMG recording experiments showed that the PVT-CeA pathway was active during wakefulness (

0.05)

CM-CeA and Rh/Re-CeA pathways were active during awakening and REM sleep (

0.05)

and the overall activity le

vel of Rh/ Re-CEA pathway was lower.

Conclusion

PVT of midline thalamus selectively projects to amygdala

PVT-CeA pathway is most active during awakening

and CM-CeA pathway is most active during wakefulness and REM sleep

and more active during REM sleep.

关键词

Keywords

references

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