Characteristics of gastric microbiota in cirrhotic patients with portal hypertension

Zhiyong MU; Yinbin ZHOU; Xiao XIAO; Jun WANG; Liangzhi WEN; Dongfeng CHEN

doi:10.16016/j.2097-0927.202205060

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Characteristics of gastric microbiota in cirrhotic patients with portal hypertension

Clinical Medicine | 更新时间：2026-01-09

- Characteristics of gastric microbiota in cirrhotic patients with portal hypertension
- Journal of Army Medical University Vol. 44, Issue 15, Pages: 1576-封3(2022)
- 作者机构：
  
  400042 重庆，陆军特色医学中心消化内科
- 作者简介：
- 基金信息：
  
  the National Nature Science Foundation for Young Scholar of China(81802459);the Project of Basic Research and Frontier Exploration of Natural Science Foundation of Chongqing(cstc2018jcyjAX0603);the Special Project of Science and Technology Innovation Capacity Promotion of Army Medical University(2019XLC3045)
- DOI：10.16016/j.2097-0927.202205060
  CLC： R372;R573;R657.34
- Received：12 May 2022，
  
  Revised：2022-05-23，
  
  Published：15 August 2022
- 稿件说明：
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Zhiyong MU, Yinbin ZHOU, Xiao XIAO, et al. Characteristics of gastric microbiota in cirrhotic patients with portal hypertension[J]. Journal of Army Medical University, 2022, 44(15): 1576-封3.
DOI：

Zhiyong MU, Yinbin ZHOU, Xiao XIAO, et al. Characteristics of gastric microbiota in cirrhotic patients with portal hypertension[J]. Journal of Army Medical University, 2022, 44(15): 1576-封3. DOI： 10.16016/j.2097-0927.202205060.

摘要

目的

分析肝硬化门脉高压症患者胃菌群的结构特征、影响因素及其与临床参数间的相关性。

方法

收集2020年11月至2021年11月于陆军特色医学中心消化内科住院接受经颈静脉肝内门体分流术(transjugular intrahepatic portosystemic shunt

TIPS)治疗的肝硬化患者的术前胃黏膜与胃液标本，并采集住院期间相关临床参数。采用16S rRNA分析胃菌群的构成及多样性，运用LEfSe寻找差异菌，冗余分析(redundancy analysis

RDA)识别胃微生物群落结构变化的主要影响因素，Spearman相关分析探讨菌群丰度与临床参数间的相关性。

结果

变形菌门(

Proteobacteria

)、放线菌门(

Actinobacteria

)、酸杆菌门(

Acidobacteria

)、绿弯菌门(

Chloroflexi

)等在胃黏膜中富集，而厚壁菌门(

Firmicutes

)、梭菌门(

Fusobacteria

)在胃液中富集。肝硬化门脉高压症患者胃黏膜物种Chao1指数显著高于胃液(

0.01)，两者间Shannon指数差异无统计学意义(

0.05)。PCoA分析表明，胃黏膜与胃液间群落构成差异有统计学意义(

0.05)。对肝硬化门脉高压性胃病(portal hypertensive gastropathy，PHG)患者胃菌群分析

显示，消化链球菌属(

Peptostreptococcus

)、二氧化碳噬纤维菌属(

Capnocytophaga

)在PHG患者胃黏膜中富集。肝硬化PHG与non-PHG患者间胃黏膜菌群α多样性与β多样性差异均无统计学意义(

0.05)。RDA分析表明，门静脉压力梯度(portal pressure gradient，PPG)是影响患者胃黏膜菌群结构变化的主要因素(

=0.683

0.001)。哈马达菌属(

Hamadaea

)、艾肯氏菌属(

Eikenella

)丰度与PPG呈正相关性(

分别为0.633、0.618；

0.05)。

结论

肝硬化门脉高压症患者胃黏膜与胃液菌群结构存在差异，但两者间物种多样性无差异；肝硬化PHG患者胃黏膜菌群构成有特征性改变。PPG是影响胃黏膜菌群结构变化的主要因素，而胃黏膜哈马达菌属及艾肯氏菌属相对丰度的升高可能与肝硬化门静脉高压相关。

Abstract

Objective

To investigate the structure characteristics

influencing factors and the correlation with clinical parameters of gastric microbiota in cirrhotic patients with portal hypertension.

Methods

Preoperative gastric mucosa and gastric juice samples were collected from cirrhotic patients undergoing transjugular intrahepatic portosystemic shunt (TIPS) in our hospital from November 2020 to November 2021

and relevant clinical parameters during hospitalization were also obtained. The composition and diversity of gastric microbiota in the patients were analyzed by 16S rRNA high-throughput sequencing. LEfSe analysis was used to search for different bacteria; Redundancy analysis (RDA) was performed to identify the main factors affecting the gastric microbiota community structure; and Spearman correlation analysis was adopted to explore the correlation between the abundance of gastric microflora and clinical parameters.

Results

Proteobacteria

Actinobacteria

Acidobacteria

and

Chloroflexi

were enriched in gastric mucosa

while

Firmicutes

and

Fusobacteria

in gastric juice. The Chao1 index of species in gastric mucosa was significantly higher than that in gastric fluid (

.01)

however

the Shannon index of community between the 2 groups was not different (

0.05). PCoA analysis showed that there was statistical difference in community composition between gastric mucosa and gastric juice (

0.05). Further analysis of the gastric microbiota indicated that

Peptostreptococcus

and

Capnocytophaga

were abundant in the gastric mucosa of patients with portal hypertensive gastropathy (PHG)

but the α diversity and β diversity of gastric mucosal flora had no significance between PHG and non-PHG patients (

0.05). RDA analysis suggested that portal pressure gradient (PPG) was the main factor affecting the community structure of gastric mucosa flora (

=0.683

0.001)

and the abundance of

Hamadaea

and

Eikenella

were positively correlated with PPG (

=0.633

0.618

respectively;

0.05).

Conclusion

The microflora structure between gastric mucosa and gastric juice is different in cirrhotic patients with portal hypertension

but there is no significant difference in the species diversity. The composition of gastric mucosal flora in patients with PHG has characteristic changes

and PPG plays an important role in the structure of gastric mucosa microbiota. The relatively increased abundance of

Hamadaea

and

Eikenella

in gastric mucosa may be associated with portal hypertension in cirrhosis.

关键词

Keywords

references

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