Effects of seawater immersion at 21 ℃ on major organ functions in rats with hemorrhagic shock

Haoyue DENG; Yu ZHU; Yue WU; Qinghui LI; Kunlun TIAN; Liangming LIU; Tao LI

doi:10.16016/j.2097-0927.202205038

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Effects of seawater immersion at 21 ℃ on major organ functions in rats with hemorrhagic shock

Monographic Reports: Treatment of war wound and traumatic shock | 更新时间：2026-01-09

- Effects of seawater immersion at 21 ℃ on major organ functions in rats with hemorrhagic shock
- Journal of Army Medical University Vol. 44, Issue 20, Pages: 2048-2053(2022)
- 作者机构：
  
  400042 重庆，陆军特色医学中心战伤休克与输血研究室，创伤、烧伤与复合伤国家重点实验室
- 作者简介：
- 基金信息：
  
  the Key Project of Logistic Scientific Research Plan of PLA(BLJ19J008)
- DOI：10.16016/j.2097-0927.202205038
  CLC： R363.22;R605.971;R835.3
- Received：10 May 2022，
  
  Revised：2022-07-18，
  
  Published：30 October 2022
- 稿件说明：
移动端阅览
Haoyue DENG, Yu ZHU, Yue WU, et al. Effects of seawater immersion at 21 ℃ on major organ functions in rats with hemorrhagic shock[J]. Journal of Army Medical University, 2022, 44(20): 2048-2053.
DOI：

Haoyue DENG, Yu ZHU, Yue WU, et al. Effects of seawater immersion at 21 ℃ on major organ functions in rats with hemorrhagic shock[J]. Journal of Army Medical University, 2022, 44(20): 2048-2053. DOI： 10.16016/j.2097-0927.202205038.

摘要

目的

观察21 ℃海水浸泡对失血休克大鼠存活情况、器官功能、组织代谢及内环境稳态的影响。

方法

128只健康雄性SD大鼠，计算机随机分组法分为: 正常对照组、40%失血休克组、正常大鼠21 ℃海水浸泡组及40%失血休克合并21 ℃海水浸泡组。建模2 h后观察各组大鼠72 h存活情况，检测各组大鼠心脏功能[肌钙蛋白T(cardiac troponin T，cTnT)]、肝脏功能[谷丙转氨酶(alanine transaminase，ALT)、谷草转氨酶(aspartate aminotransferase，AST)]、肾脏功能[血尿素(blood urea nitrogen，BUN)、血肌酐(serum creatinine，SCr)]；组织代谢及内环境指标的变化情况。

结果

40%失血休克组和21 ℃海水浸泡组均较对照组大鼠血浆渗透压(plasma osmolality

OSM)及Na

、K

、Cl

浓度增加，肺脑含水量升高，肺脑Na

-ATP酶活性降低，心脏、肝脏和肾脏功能损伤指标升高，死亡率增加。而40%失血休克合并21℃海水浸泡可加重40%失血休克引起的损伤，表现为血浆OSM、Na

、K

、Cl

进一步升高，肺脑含水量进一步升高，肺、脑Na

-ATP酶活性进一步降至(3.81±0.30)U/mg、(7.17±0.51)U/mg，心脏、肝脏和肾脏功能进一步恶化，体现为cTnT、ALT、AST、BUN、SCr分别增至(7.25±0.66)μg/L、(118.60±13.29)U/L、(455.95±46.76)U/L、(19.74±2.12)mmol/L、(56.73±4.08)μmol/L，存活率降至18.75%，中位存活时间缩短至10.50 h。

结论

21 ℃海水浸泡可加剧40%失血休克大鼠内环境紊乱及组织代谢障碍，导致多器官功能损伤加重，进而促使死亡率升高。

Abstract

Objective

To determine the effects of seawater immersion at 21 ℃ on survival

organ functions

tissue metabolism and homeostasis in rats with hemorrhagic shock.

Methods

A total of 128 healthy male SD rats were randomly divided into 4 groups: normal control group (NC)

40% hemorrhagic shock group (HS)

normal rats with 21 ℃ seawater immersion (2 h) group (SI)

and 40% hemorrhagic shock rats combined with 21 ℃ seawater immersion (2 h) group (HS+SI). The survival rate in each group was observed for 72 h in 2 h after modeling

the changes of cardiac function [cardiac troponin T (cTnT)]

liver function [alanine transaminase (ALT) and aspartate aminotransferase (AST) levels]

renal function [blood urea nitrogen (BUN) and serum creatinine (SCr) levels]

tissue metabolism and internal environment were detected in each group.

Results

As compared with the NC group

the plasma osmolality (OSM)

concentrations of Na

and water contents in the lung and brain were increased

while the Na

-ATPase activity in the lung and brain was

decreased in the HS and SI groups. With the elevation of the damage indexes in heart

liver and kidney functions

the mortality was increased as well in the HS and SI groups. Combination of 21 ℃ seawater immersion resulted in more severe damages induced by simple 40% hemorrhagic shock

shown as further increases in plasma OSM

and Cl

concentrations

and water contents of the lung and brain

as well as further declined Na

-ATPase activity in the lung (3.81±0.30 U/mg) and brain (7.17±0.51 U/mg) (

0.05). Serious deterioration was observed in the functions of the heart

liver and kidney

with levels of cTnT

ALT

AST

BUN and SCr elevated to 7.25±0.66 μg/L

118.60±13.29 U/L

455.95±46.76 U/L

19.74±2.12 mmol/L and 56.73±4.08 μmol/L

respectively (

0.05). The survival rate was reduced to 18.75%

and the median survival time was shortened to 10.50 h in the rats of the HS+SI group.

Conclusion

Seawater immersion at 21℃ exacerbates disorders of the internal environment and tissue metabolism in 40% hemorrhagic shock rats

leading to aggravated multiple organ injury and thus increased mortality.

关键词

Keywords

references

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