Improvement of noninvasive electrostatic field stimulation on lipopolysaccharide-induced neurocognitive impairment mice

Guoliang WEI; Cong WANG; Shuqing SUN; Guisong LIU; Xuelong TIAN; Xing WANG; Huizhong WEN

doi:10.16016/j.2097-0927.202204192

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Improvement of noninvasive electrostatic field stimulation on lipopolysaccharide-induced neurocognitive impairment mice

Neurosciences | 更新时间：2026-01-09

- Improvement of noninvasive electrostatic field stimulation on lipopolysaccharide-induced neurocognitive impairment mice
- Journal of Army Medical University Vol. 44, Issue 22, Pages: 2237-2245(2022)
- 作者机构：
  
  1.400044 重庆，重庆大学生物工程学院
  2.400038 重庆，陆军军医大学(第三军医大学)基础医学院神经生物学教研室，重庆市神经生物学重点实验室
- 作者简介：
- 基金信息：
  
  the Project of Basic Research and Frontier Technology of Chongqing(cstc2018jcyjAX0506)
- DOI：10.16016/j.2097-0927.202204192
  CLC： R454.1;R741.05;R749.12
- Received：26 April 2022，
  
  Revised：2022-07-07，
  
  Published：30 November 2022
- 稿件说明：
移动端阅览
Guoliang WEI, Cong WANG, Shuqing SUN, et al. Improvement of noninvasive electrostatic field stimulation on lipopolysaccharide-induced neurocognitive impairment mice[J]. Journal of Army Medical University, 2022, 44(22): 2237-2245.
DOI：

Guoliang WEI, Cong WANG, Shuqing SUN, et al. Improvement of noninvasive electrostatic field stimulation on lipopolysaccharide-induced neurocognitive impairment mice[J]. Journal of Army Medical University, 2022, 44(22): 2237-2245. DOI： 10.16016/j.2097-0927.202204192.

摘要

目的

探究非侵入式静电场刺激(noninvasive electrostatic field stimulation

NEFS)对脂多糖(lipopolysaccharide，LPS)诱导的神经认知障碍小鼠的改善作用。

方法

将48只3月龄雌性C57小鼠分为CTL组、NaCl+NEFS组、LPS+SHAM组、LPS+NEFS组，每组12只。LPS+NEFS组和LPS+SHAM组都以腹腔注射LPS(2 mg/kg)的方式建立神经认知障碍模型，建模后，连续进行4 d的NEFS治疗，其中LPS+NEFS组接受160 kV/m的静电场刺激(30 min/d)而LPS+SHAM组只接受0 kV/m的假刺激(3 s/d)。NaCl+NEFS组注射等量0.9%生理盐水代替LPS并进行160 kV/m的静电场刺激；CTL组不做任何处理。NEFS后，用T迷宫(T-maze)实验和新物体识别(new object recognition

NOR)实验观察小鼠的认知能力；用免疫组化技术检测小鼠海马神经元的数量；用免疫荧光技术检测小鼠海马星形胶质细胞的变化。

结果

与CTL组比较，LPS+SHAM组在T-maze和NOR中的学习记忆能力显著减弱(

0.05)，且海马区神经元的数目显著减少而活化的星形胶质细胞的数目显著增加(

0.05)。而与LPS+SHAM组比较，LPS+NEFS组在T-maze和NOR中的学习记忆能力显著提高(

0.05)，且海马区神经元的数目也显著增加(

0.05)，星形胶质细胞的增生被显著抑制(

0.05)。

结论

腹腔注射2 mg/kg的LPS可以建立神经认知障碍模型，NEFS对LPS诱导神经认知障碍小鼠起到改善作用。

Abstract

Objective

To explore the improvement of noninvasive electrostatic field stimulation (NEFS) on neurocognitive impairment mice induced by lipopolysaccharide (LPS).

Methods

Forty-eight female C57 mice aged 3 months were divided into the CTL group

the NaCl+NEFS group

the LPS+SHAM group and the LPS+NEFS group

with 12 mice in each group. Both the LPS+NEFS group and the LPS+SHAM group were injected with 2 mg/kg LPS intraperitoneally to establish the mouse model of neurocognitive impairment

and after the model was constructed

they were treated with NEFS for 4 d

during which the LPS+NEFS group received an electrostatic field of 160 kV/m for 30 min/d while the LPS+SHAM group were only stimulated by an electrostatic field of 0 kV/m for 3 s/d. The NaCl+NEFS group was injected with the same amount of 0.9% normal saline instead of LPS and stimulated by electrostatic field (160 kV/m); The CTL group was a no-treatment group. After NEFS

the cognitive ability of mice was observed by T maze experiment (T-maze) and the new object recognition (NOR) experiment. Immunohistochemical method was used to detect the number of hippocampal neurons in mice

and immunofluorescence method was used to detect the changes of hippocampal astrocytes.

Results

Compared with the results of behavioral experiment and morphological observation of the CTL group

the learning and memory abilities of the LPS+SHAM group in T-maze and NOR was significantly weakened (

0.05)

and the number of neurons in hippocampus decreased significantly while the number of activated astrocytes increased significantly (

0.05). Furthermore

compared with the LPS+SHAM group

the learning and memory abilities of the LPS+NEFS group in T-maze and NOR was significantly improved (

0.05)

and the number of neurons in hippocampus was also significantly increased (

0.05)

and the proliferation of astrocytes was significantly inhibited (

0.05).

Conclusion

Intraperitoneal injection of 2 mg/kg LPS can establish neurocognitive impairment model

and NEFS has protective effect on LPS-induced neurocognitive impairment in mice.

关键词

Keywords

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